Advances in heparin structural analysis by chromatography technologies

Ouyang, Yilan; Lin, Yi; Qiu, Luyun; Zhang, Zhenqing

doi:10.3724/sp.j.1123.2022.07020

Cited by 2 publications

(2 citation statements)

References 85 publications

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“…[8][9][10] In addition to its use in countering infections, 11,12 heparin molecules serve as nanocarriers in drug delivery systems. 13,14 The highly complex structure of heparin, still under investigation for its exact composition, 15 adds to its diverse range of medical uses.…”

Section: Introductionmentioning

confidence: 99%

Exploring single-molecule interactions: heparin and FGF-1 proteins through solid-state nanopores

Thyashan,

Ghimire,

Lee

et al. 2024

Nanoscale

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Section: Introductionmentioning

confidence: 99%

Exploring single-molecule interactions: heparin and FGF-1 proteins through solid-state nanopores

Thyashan,

Ghimire,

Lee

et al. 2024

Nanoscale

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“…The diverse structural types of saccharides provide the foundation for their various biological functions. Therefore, in-depth research on saccharides has placed great emphasis on the analysis of saccharide structure to explore their biological functions [7][8][9][10], with the separation and analysis of saccharides becoming fundamental approaches to structural characterization [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

A low‐cost efficient online derivatization system for the determination of saccharides by high‐performance liquid chromatograph‐ultraviolet detector

Jiang,

Wang,

et al. 2023

J of Separation Science

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In this study, a low‐cost efficient online derivatization system was developed which allows for the detection of various types of mono‐ and oligo‐saccharides only utilizing high‐performance liquid chromatography (HPLC)‐ultraviolet detector (UV) system. In the proposed method, phenylhydrazine was used as the derivatization reagent and directly spiked in the mobile phase, allowing for the separation and detection of mono‐ and oligosaccharides in an accessible instrument system (HPLC‐UV). And the online derivatization design of the proposed method has significantly reduced the potential harm of derivatization reagents to the analysts. Furthermore, critical chromatographic parameters were optimized via the Box‐Behnken design strategy, culminating in the ideal response for saccharides. Finally, the methodology validation of the proposed method was conducted. The proposed method showed satisfactory linear ranges with acceptable correlation coefficients (R2 > 0.99), outstanding accuracy (Recovery: 95.3%–105.6%), high intra‐day precision (relative standard deviation [RSD]: 1.4%–7.1%) and inter‐day precision (RSD: 2.0%–7.4%). The robustness and ruggedness of the proposed method were proved as the recovery values in the range of 95.0%–104.6% and 95.1%–104.8% for robustness and ruggedness, respectively. These satisfactory validation results confirm the applicability and reliability of the proposed method for the analysis of saccharides in various complex real‐world samples.

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Advances in heparin structural analysis by chromatography technologies

Cited by 2 publications

References 85 publications

Exploring single-molecule interactions: heparin and FGF-1 proteins through solid-state nanopores

Exploring single-molecule interactions: heparin and FGF-1 proteins through solid-state nanopores

A low‐cost efficient online derivatization system for the determination of saccharides by high‐performance liquid chromatograph‐ultraviolet detector

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