Glycoproteins from the surface of metaphase cells

Glick, Mary Catherine; Buck, Clayton A.

doi:10.1021/bi00725a016

Cited by 69 publications

(10 citation statements)

References 12 publications

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“…It has been shown that, in certain cells undergoing rapid division, e.g. tumour cells, the structures of these glycosubstances are altered (Hakomori & Murakami, 1968;Glick & Buck, 1973;Dabelsteen & Pindborg, 1973). It is not known whether this is a consequence of the rapid growth or whether the modified structure of these glycosubstances is a primary cause of the rapid growth, possibly by altering contact inhibition of cells (Burger & Noonan, 1970) or the membrane permeability, thus allowing entry of important metabo¬ lites (Holley, 1972).…”

Section: Tions Discussionmentioning

confidence: 99%

An Investigation of Enzymes and Hormone-Binding Proteins in the Luminal Fluid of the Bovine Uterus

Roberts¹,

Parker²

1974

Reproduction

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Section: Tions Discussionmentioning

confidence: 99%

An Investigation of Enzymes and Hormone-Binding Proteins in the Luminal Fluid of the Bovine Uterus

Roberts¹,

Parker²

1974

Reproduction

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“…Differences in the chemical makeup of the cell surface of normal and tumor cells have revealed that glycosylated macromolecules, glycoproteins and glycolipids, show consistent patterns of alteration (1). Warren, Glick, and their associates (2)(3)(4)(5) and, more recently, others (6) have reported that tumor-characteristic sialofucosyl glycopeptides, present only in trace amounts in normal cells, are considerably elevated in transformed cells. Glycopeptide alterations have been demonstrated in tumor cells obtained from peripheral blood of patients with active leukemia (untreated patients with at least 80% leukemic cells in the white cell fraction) (7).…”

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confidence: 99%

Glycolipid-bound sialic acid in serum: increased levels in mice and humans bearing mammary carcinomas.

Kloppel¹,

Keenan²,

Freeman³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

104

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In mice bearing transplantable mammary carcinomas, serum levels of sialic acid-containing glycolipids were elevated 2.5-fold in pooled serum samples from which gangliosides were purified by column chromatography. A method is also described by which ganglioside content was estimated on as little as 1.0 ml of whole blood to permit studies with individual tumor-bearing mice and age-and litter-matched controls. Using this method, we observed similar elevations in ganglioside levels that were independent of age and sex of the animal and appeared in advance of palpable tumors. Following excision of the tumors, the glycolipid sialic acid values dropped below control levels and remained there. Serum sialic acid of the glycolipid fraction was elevated nearly 2-fold in human carcinoma patients and appeared to decline after surgery. taneous implantation of tumor fragments into male or female syngeneic recipients. Age-matched controls of the same strain were injected with an equivalent amount of saline but received no tumor implant and were otherwise treated exactly as the principals. Tumor masses were examined for degree of vascularization and then were excised and weighed. Determination of Gangliosides. Groups of 25 carcinomabearing mice were exsanguinated 60 days after implantation of tumor fragments. Sera were pooled following centrifugation. Gangliosides were extracted with chloroform/methanol and further purified by chromatography on DEAE-Sephadex and Unisil silicic acid (16). Sialic acid assays (17) were on purified gangliosides. By this method recovery of [6-3H]galactose-Nacetylgalactosamine-(N-acetylneuraminic acid)-galactoseglucose-ceramide ([3H]GM1) added to samples averaged 96%.Individual mice bearing tumors were exsanguinated at 3-90 days after implantation of tumors along with litter-matched control mice as described above. The sera (0.5-1 ml) obtained following centrifugation were extracted with chloroform/ methanol (1:1 vol/vol) overnight. The extracts were filtered and the residue was extracted a second time with chloroform/ methanol (2:1) overnight. The combined extracts were concentrated and adjusted to 3 ml. To precipitate sialic acid-containing lipo-and glycoproteins (not elevated in sera of tumor bearers), 100 ,ul of a 0.1% solution of tripotassium citrate (18) was added and the resulting precipitate was removed by centrifugation. The supernatant after citrate treatment was analyzed for sialic acid (17) using values calculated by the Warren (17) formula to correct for variation due to unspecific absorbance. With this method recovery of [3H]GM1 added to serum samples averaged 92%. RESULTS Animal modelThe level of total gangliosides, determined on the basis of sialic acid, was elevated about 2.5 times above control values in sera of both male and female recipient mice carrying a transplantable mammary carcinoma of spontaneous origin (Table 1). Similar elevations were observed at 11, 21, and 35 days after transplantation; at 11 days palpable tumor masses were not detected. These increases were no...

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“…Differences in surface glycoprotein composition between cells in different stages of cell growth have been reported (Buck, Glick & Warren, 1971 ;Glick & Buck, 1972;Muramatsu, Atkinson, Nathenson & Ceccarini, 1973). We cannot exclude the possibility that differences in intercellular attractive forces might also exist.…”

Section: Discussionmentioning

confidence: 84%

Cell size and mutual cell adhesion

1976

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The strength of the long-range electrostatic repulsion forces on HeLa cells is measured by agglutinative titration using low molecular weight polylysine (M.W. 11,000). Repulsion forces, found to be present on the smaller HeLa cells from density-inhibited suspension cultures, are weakened by incubation of the cells in hypotonic NaCl solutions. Repulsion forces, found to be absent on the larger cells from fast growing cultures, can be induced on these cells by incubation in hypertonic NaCl solutions. Both effects of anisotonicity are reversible, and disappear on restoration of the medium to normal tonicity. Induction of repulsion forces on fast growing cells is prevented by previous treatment of the cells with neuraminidase. Neuraminidase also abolishes repulsion on density-inhibited cells. It is proposed that alterations of the cell size, produced by anisotonicity or occurring during growth in isotonic suspension medium, affect mutual cell adhesiveness by modifying the strength of the repulsion forces generated by cell surface sialic acids.

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Glycoproteins from the surface of metaphase cells

Abstract: ANDCELL DIVISION methane must be the first intermediate in the biosynthesis of uroporphyrinogen III. Both uroporphyrinogens must proceed through different pathways after the first porphobilinogen units became bound to the deaminase.

Cited by 69 publications

References 12 publications

An Investigation of Enzymes and Hormone-Binding Proteins in the Luminal Fluid of the Bovine Uterus

An Investigation of Enzymes and Hormone-Binding Proteins in the Luminal Fluid of the Bovine Uterus

Glycolipid-bound sialic acid in serum: increased levels in mice and humans bearing mammary carcinomas.

Cell size and mutual cell adhesion

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