An Investigation of Enzymes and Hormone-Binding Proteins in the Luminal Fluid of the Bovine Uterus

Roberts, Geoffrey; Parker, Janice

doi:10.1530/jrf.0.0400305

Cited by 32 publications

(22 citation statements)

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“…These proteins were not observed in oviducal fluid taken from animals in oestrus. They migrate at a similar rate to the uterine proteins described in a previous paper (Roberts & Parker, 1974a) Histochemical examination of bovine oviducal epithelium (Björkman & Fredricsson, 1966;Fredricsson, 1969) The level of glycosidase activity in the oviducal fluid is much lower than that found in bovine uterine fluid (Roberts & Parker, 1974b) and the levels at oestrus are similar to those found in serum. The activities of /J-N-acetylgalactosaminidase and /?-N-acetylglucosaminidase increased at mid-cycle, probably under the influence of progesterone, but during this stage of the cycle it is unlikely that the enzymes from the oviduct fluid would be of importance in embryonic development.…”

Section: Discussionsupporting

confidence: 56%

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Proteins in the Luminal Fluid From the Bovine Oviduct

Roberts¹,

Parker²,

Symonds³

1975

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Section: Discussionsupporting

confidence: 56%

“…Double diffusion in agar was carried out according to the method of Ouchterlony (1958) (Roberts & Parker, 1974b).…”

Section: Gel Filtrationmentioning

confidence: 99%

Proteins in the Luminal Fluid From the Bovine Oviduct

Roberts¹,

Parker²,

Symonds³

1975

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“…The elevated activities of a-L-fucosidase, ß-D-galactosidase, -D-glucosidase, ß-N-acetylgalactosaminidase and ß-N-acetylglucosaminidase in ovine uterine fluid parallel the activities of these glycosidases in bovine uterine fluid (Roberts & Parker, 1974b), but the activities were higher in the sheep. As in the cow (Roberts, Parker & Symonds, 1975), the activities of all the glycosidases in sheep were much less in oviducal fluid than in uterine fluid.…”

Section: Discussionmentioning

confidence: 79%

“…The enzymes (a-L-fucosidase, ß-L-fucosidase, -D-galactosidase, ß-D-galactosidase, -D-glucosidase, ß-D-glucosidase, ß-A^-acetylgalactosaminidase, ß-7Y-acetylglucosaminidase, rx-D-mannosidase and ß-D-mannosidase) were assayed at pH 50 using the corresponding /»-nitrophenyl glycosides as described previously (Roberts & Parker, 1974b).…”

Section: Assaysmentioning

confidence: 99%

Macromolecular components of genital tract fluids from the sheep

Roberts¹,

Parker²,

Symonds³

1976

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The proteins and glycosidases in the luminal fluid from the genital tract of ewes at different stages of the oestrous cycle and early pregnancy were studied. Most of the proteins in oviducal fluid and uterine fluid were serum proteins, but an increased amount of a glycoprotein excluded from Sephadex G-200 was detected in oviducal fluid at oestrus. Small amounts of uterine-specific proteins were detected in uterine fluid by polyacrylamide gel electrophoresis and isoelectric focusing. The number of these varied according to the stage of pregnancy. The activities of several glycosidases were elevated in uterine fluid when compared with serum; beta-N-acetylgalactosaminidase and beta-N-acetylglucosamindase were particularly active. Ovine cervical mucus was shown to contain an epithelial glycoprotein which had a composition similar to that of the glycoproteins isolated from human and bovine cervical mucus.

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“…ex-L-Fucosidase (BC 3.2.1.51), poD-galactosidase (BC 3.2.1.23), p-N-acetylgalactosaminidase (BC 3.2.1.53) and p-N-acetylglucosaminidase (EC 3.2.1.30) were assayed according to the method of Roberts and Parker (1974). The amount of vaginal flushings (700-g supernatant) used varied from 50 to 200 p,l, containing approximately 6()"100 p,g protein.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Enzyme Activities and Protein and Carbohydrate Concentrations in Cervical Secretions at Dioestrus in Normal Ewes and Ewes With Permanent Phytooestrogenic Infertility

Tang¹,

Adams²

1978

Aust. Jnl. Of Bio. Sci.

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Cervical secretions of clover-affected and control ewes in the luteal phase of the ovarian cycle were obtained by flushing the anterior vagina. The flushings were analysed for proteins, carbohydrates and enzyme activities, and were found to be similar to the secretions of the normal ovine uterus. There was significantly more protein, carbohydrate and acid-soluble glycoprotein but less alkaline phosphatase, N-acetylglycosidases (EC 3.2.1.30 and 3.2.1.53) and ribonuclease I in the vaginal flushings of clover-affected ewes.The observed changes were not due to more inflammation in the cervix of clover-affected ewes as there were fewer bacteria, leukocytes and epithelial cells and no elevation of lysozomal enzyme activities in their flushings. It is suggested that the cervix of the clover-affected ewe behaves as though under a stronger than normal oestrogenic stimulation during dioestrus. IntroductionEwes rendered permanently infertile by prolonged ingestion of oestrogenic clover produce abnormal cervical mucus which prevents normal sperm transport and fertilization (Lightfoot et al. 1967;Smith 1971). Histological changes of the cervices of these ewes have also been observed by Heydon and Adams (1977). A test on cervical secretions could be expected to be a useful diagnostic method for detecting permanent clover infertility.Adams (1967a) found that mucus from clover-affected ewes had a spinnbarkeit value significantly lower than that from control ewes. Although the mucus spinnbarkeit test was subsequently found to be useful for assessing lowered reproductive performance in ewes exposed to oestrogenic pasture (Adams 1977), it has limitations. Spinnbarkeit varies with the time of the breeding season so that adjustment is needed to compare results obtained at different times. Cervical mucus is abundant only when the ewe is in oestrus and even then some ewes produce so little that collection of an adequate sample is impossible. Because of these limitations it is worthwhile to seek other diagnostic methods and one possible area is the biochemical analysis of cervical secretions during the luteal phase of the reproductive cycle.In the present experiment proteins, carbohydrates and some enzymes, including lysozomal enzymes, in the. luteal-phase cervical secretions obtained by flushing the anterior vagina of clover-affected and control ewes were assayed and compared.

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An Investigation of Enzymes and Hormone-Binding Proteins in the Luminal Fluid of the Bovine Uterus

Abstract: Summary. Saline

Cited by 32 publications

References 20 publications

Proteins in the Luminal Fluid From the Bovine Oviduct

Proteins in the Luminal Fluid From the Bovine Oviduct

Macromolecular components of genital tract fluids from the sheep

Enzyme Activities and Protein and Carbohydrate Concentrations in Cervical Secretions at Dioestrus in Normal Ewes and Ewes With Permanent Phytooestrogenic Infertility

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