Proteins in the Luminal Fluid From the Bovine Oviduct

Roberts, G P; Parker, Janice; Symonds, H.W.

doi:10.1530/jrf.0.0450301

Cited by 48 publications

(49 citation statements)

References 22 publications

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“…b-N-Acetyl-glucosaminidase activity has been already described in the oviduct from other species (Roberts et al 1975, 1976, Tulsiani et al 1996, Droba et al 2005, and in our study the highest activity was at the late follicular phase. Regarding oviductal b-N-acetyl-galactosaminidase activity, no studies are available and we observed its maximum activity at the early follicular phase.…”

Section: Discussionsupporting

confidence: 82%

“…All these events take place in the oviduct and have been the aim of studies to increase our knowledge about the molecular basis of reproductive functions, but, surprisingly, we lack of precise studies about the activities of the different glycosidases in the OF. The exception could be found in some early reports from Roberts et al (1975Roberts et al ( , 1976 showing low activities for a-…”

Section: Introductionmentioning

confidence: 75%

“…It is obvious then to suppose that oviductal glycosidases, if present and active at a physiological pH, may have a role in fertilization, as has been reported for other glycosidases in sperm membranes (Tulsiani et al 1989, Cornwall et al 1991 or epididymal fluid (Skudlarek et al 1993). However, and surprisingly, no studies about enzymatic composition in such an important biological fluid as the OF have been carried out in mammals, except some old studies in cows and sheep (Roberts et al 1975(Roberts et al , 1976 or more recently in the hamster (Tulsiani et al 1996). The results from this study show detailed and novel data about the glycosidase activity at physiological pH and protein concentration in the porcine OF.…”

Section: Discussionmentioning

confidence: 99%

“…As an example, b-galactosidase optimally cleaves the synthetic substrate at acidic pH, but it shows maximum activity toward glycoprotein substrates in a neutral environment. This may explain how the b-galactosidase or perhaps other 'acidic' glycosidases could be functional in the different extracellular sites where they have been detected, such as blood (Tulsiani & Touster 1981), sperm membranes (Tulsiani et al 1989, Cornwall et al 1991, epididymal luminal fluid (Skudlarek et al 1993), and fluids from the female reproductive tract (Roberts et al 1975, 1976, Tulsiani et al 1996.…”

Section: Introductionmentioning

confidence: 99%

“…However, precise studies about glycosidase activity in the OF during the estrous cycle in mammalian species such as the pig or cow, where reproductive technologies are usually and widely performed, are scarce (Roberts et al 1975, 1976, Tulsiani et al 1996. On the contrary, studies on several glycosidase activities in sperm membranes (Tulsiani et al 1989, Cornwall et al 1991 and epididymal fluid (Skudlarek et al 1993) are available.…”

Section: Introductionmentioning

confidence: 99%

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Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle

Carrasco

Romar

Sánchez³

et al. 2008

Reproduction

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Sperm-oocyte binding and gamete-oviductal epithelium interactions are carbohydrate-mediated events occurring in the oviductal fluid (OF). Thus, knowledge about the activities of glycosidases (enzymes catalyzing hydrolytic cleavage of terminal sugar residues) in this milieu would help us understand the molecular mechanisms involved in these events. This work was carried out to investigate the glycosidase activity, protein content, and volume of OF collected from gilts and sows. Oviducts were classified into four phases of the estrous cycle (early follicular, late follicular, early luteal, and late luteal) based on the appearance of the ovaries. OF was aspirated, centrifuged, measured for volume, and frozen until assay. Substrates conjugated to 4-methylumbelliferyl were used to screen the activities of seven different glycosidases at physiological pH (7.2). a-L-Fucosidase and b-N-acetyl-glucosaminidase activities increased at the late follicular phase to decrease after ovulation. b-D-Galactosidase, a-D-mannosidase, and b-N-acetyl-galactosaminidase showed higher activities at the early follicular phase, which decreased after ovulation. N-Acetyl-neuraminidase and a-D-galactosidase did not show activity at any phase of estrous cycle neither in sows nor in gilts at pH 7.2, although it did at acidic pH (4.4) in the follicular and luteal phase samples. Total protein also changed during the cycle showing the maximum secretion at the late follicular phase (2118.6G200.7 mg/oviduct). The highest volumes of OF were collected from the oviducts at the late follicular phase (50.7G1.3 ml/oviduct). These results indicate that OF from sows and gilts shows glycosidase activity varying throughout the estrous cycle suggesting a role of these enzymes in carbohydrate-mediated events. Reproduction (2008) 136 833-842

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle

Carrasco

Romar

Sánchez³

et al. 2008

Reproduction

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Expression of vascular endothelial growth factor (VEGF) and its corresponding receptors (flt-1 and flk-1) in the bovine oviduct

et al. 1999

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Vascular endothelial growth factor (VEGF) functions as a potent angiogenic protein as well as in regulating permeability. Reverse transcription‐polymerase chain reaction (RT‐PCR) and ribonuclease protection assay (RPA) were used to show that the bovine oviduct expresses VEGF and its two receptors flk‐1 and flt‐1. Expression of VEGF was relatively stable during the estrous cycle. In contrast, both receptor transcripts showed cycle‐dependent variations with significantly increased flt‐1 mRNA amounts before ovulation. Immunohistochemical studies localized VEGF mainly on the epithelial surface of oviducts. Protein concentrations of VEGF in oviductal flushings were significantly higher (mean ± SEM: 2.8 ± 0.8 ng/ml) during the pre‐ovulatory phase when compared with the other estrous cycle stages (1.0 ± 0.25 ng/ml). In conclusion, all components of a functional VEGF‐system in the bovine oviduct were found to undergo specific modulations during the cycle. We suggest that VEGF may be involved in creating an optimal local environment for fertilization or the developing embryo by modulating permeability within the bovine oviduct. Mol. Reprod. Dev. 53:376–383, 1999. © 1999 Wiley‐Liss, Inc.

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Culture of bovine oviduct epithelial cells (BOEC)

Walter

1995

Anat. Rec.

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Proteins in the Luminal Fluid From the Bovine Oviduct

Cited by 48 publications

References 22 publications

Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle

Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle

Expression of vascular endothelial growth factor (VEGF) and its corresponding receptors (flt-1 and flk-1) in the bovine oviduct

Culture of bovine oviduct epithelial cells (BOEC)

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