Glycopolymer-coated iron oxide nanoparticles: shape-controlled synthesis and cellular uptake

Li, Xiao; Bao, Meimei; Weng, Yuyan; Yang, Kai; Zhang, Weidong; Chen, Gaojian

doi:10.1039/c4tb00852a

Cited by 44 publications

(33 citation statements)

References 52 publications

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“…[1][2][3] Uptake of engineered nanoparticles into cells tends to be size, shape and charge-dependent. 2,4,5 The properties of surface materials are also of importance for the targeted delivery of particles into specific cells, or even defined organelles. 5,6 Immobilization of carbohydrates, especially glycopolymers, onto magnetic or fluorescent nanoparticles has previously been exploited for pathogen detection and cell imaging.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of well-defined catechol polymers for surface functionalization of magnetic nanoparticles

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Synthesis of well-defined catechol polymers for surface functionalization of magnetic nanoparticles

et al. 2016

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“…8B) Compared to their spherical counterparts, capsules with an ellipsoidal or other non-spherical shape have many additional advantages such as enhanced cargo loading efficiency for drug delivering, improved cell uptake in the cases of cancer treatment and enhanced packing efficiency in solid materials as indicated by previous works. [13][14][15] Relative to the current work, we think the advantage of enhanced packing efficiency in solid materials is very benecial. In the format of dried powders in which particles are randomly packed, ellipsoidal particles can randomly pack more densely and achieve a volume fraction (4) up to 0.74, higher than 0.64 of spherical ones.…”

Section: 33mentioning

confidence: 99%

“…5,6,[8][9][10][11][12] It is noted that most of PDA capsules prepared so far have a spherical shape, [4][5][6][7]11 while non-spherical ones may have additional advantages in cargo loading, cell uptake and enhanced packing efficiency in energetic materials. [13][14][15] However, preparation of non-spherical polymeric capsules is generally challenging.…”

Section: Introductionmentioning

confidence: 99%

Anisotropic polydopamine capsules with an ellipsoidal shape that can tolerate harsh conditions: efficient adsorbents for organic dyes and precursors for ellipsoidal hollow carbon particles

Zhang

et al. 2017

RSC Adv.

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Polymeric capsules often buckle, collapse or even break when being processed in the dried state into other materials under high temperature and pressure due to moderate mechanical rigidity. In the case of nonspherical capsules, to keep their precious anisotropic morphology intact under harsh conditions is even more challenging since the whole surface of such kinds of capsules does not experience the same stress or strain due to the different surface curvatures. In the current work, we reported a strategy to prepare polydopamine (PDA) capsules with an ellipsoidal shape and enhanced mechanical rigidity using polystyrene ellipsoids as the sacrificial anisotropic templates. Bio-inspired oxidation induced selfpolymerization of dopamine can form conformal PDA coatings on polystyrene ellipsoids of various aspect ratios and sizes. Several strategies have been exploited to increase the thickness of the PDA shell, among which, iterating PDA coating produces ellipsoidal PDA capsules with a thick and robust shell.These ellipsoidal PDA capsules can survive carbonization at temperatures as high as 800 C and were directly turned into N-doped carbon capsules with a well-defined ellipsoidal shape, excluding the necessity of removing the sacrificial templates after carbonation. Furthermore, the rigid PDA ellipsoidal capsules are efficient adsorbents for organic dyes in contaminated water and have impressive adsorption efficiencies as high as 200 mg g À1.

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“…As shown in Figure A, the addition of ConA to CpG‐GM@AuNPs decorated with mannose and glucose gave rise to aggregation in PBS. Notably, in case of CpG‐G@AuNPs, obvious aggregation was not observed (Figure B) due to the relatively weak binding ability in PBS, which indicated that the CpG‐GM@AuNPs are attractive candidates for specific targeting to APCs. In fact, antigen and adjuvant internalization by APCs is important for the efficacy of vaccines.…”

Section: Methodsmentioning

confidence: 99%

“…To evaluate the cell uptake of glycoadjuvant@AuNPs by APCs, Raw 264.7 macrophages were incubated with CpG‐GM@AuNPs or CpG‐G@AuNPs. The number of NPs ( N ) uptaken per cell can be calculated using inductively coupled plasma‐atomic emission spectroscopy (ICP‐AES) technique . As shown in Figure D, compared to CpG‐G@AuNPs, DC‐targeting CpG‐GM@AuNPs showed significantly enhanced cell uptake by macrophages.…”

Section: Methodsmentioning

confidence: 99%

One‐Pot, Multicomponent Strategy for Designing Lymphoseek‐Inspired Hetero‐Glycoadjuvant@AuNPs

Liu

Wen

Shen

et al. 2019

Macromol. Rapid Commun.

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transported to draining lymph nodes. The second approach is based on the activation of antigen-presenting cells (APCs) to induce T cell. [9,10] Recently, inspired by the lymphoseek [11] (which is a mannose-conjugated, dextran-based LN imaging agent, acting as receptor-targeted radiotracer in breast cancer and melanoma patients), we have first developed LN-targeting adjuvant by conjugating toll-like receptor (TLR) [9] agonists, unmethylated Cytosinephosphate-Guanosine (CpG) to natural dextran. [12] Although dextran-functionalized adjuvant could not increase adjuvant internalization, they could distinctly enhance CD8 + T-cell responses, and improve cancer immunotherapy. [12] More importantly, dextran-based adjuvants could not only activate tumor-specific CTL responses, but also inhibit myeloid-derived suppressor cells (MDSCs) in tumor immune microenvironment. [13] Many works indicate that binding between C-type lectin receptors (CLRs) and carbohydrate ligands [14][15][16][17][18][19] is important not only for the induction of T-cell responses, but also for the inhibition of immunosuppressive microenvironment. [13] Therefore, lymphoseek-inspired vaccine should have advantages of promoting vaccine internalization, potentiating CD8 + T-cell responses and reducing the immunosuppressive activity, and become a promising candidate for nextgeneration cancer vaccine.Much effort has been devoted to developing new methodologies for the design and preparation of multifunctional adjuvant, which is the most challenging work. To obtain bioactive adjuvant, simple and highly efficient reaction should be used to formulate adjuvant. To date, most of the works use "grafting to" method to generate target adjuvant. [20][21][22][23] Lynn et al. attached small-molecule TLR-7/8a agonists to hydrophilic polymers to form polymer-TLR-7/8a conjugates by "grafting to" strategy. [24] Recently, inspired by quinone chemistry, we have also introduced "thiol-quinone" reaction to attach thiolated CpG to NPs for fabricating pathogen-mimetic [25][26][27] adjuvants by the "grafting to" method. [28] Besides, few works employ multicomponent strategy to design adjuvants. It is well known that onepot multicomponent strategy offers obvious advantages of simplicity and high efficiency over traditional chemical reactions. Moreover, one-pot strategy is more convenient to obtain high concentration adjuvants. Herein, we report a novel multicomponent strategy to develop lymphoseek-inspired adjuvants in one-pot. First, photo-induced reversible addition-fragmentation Glycoadjuvants Searching for vaccines (antigen and adjuvant) with easy preparation and strong T-cell response are crucial for antitumor immunity. In this work, to design lymphoseek-inspired vaccine possessing the abilities of promoting vaccine internalization and enhancing CD8 + T-cell responses, a simple multicomponent strategy is successfully utilized to fabricate lymph node and dendritic cell dual-targeting glycoadjuvant@AuNPs in one pot, where three different components, catechol-containing glycopol...

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Glycopolymer-coated iron oxide nanoparticles: shape-controlled synthesis and cellular uptake

Abstract: Serum-stable glyco-nanoparticles with controlled shape were easily obtained and exhibit shape-dependent cell uptake behaviors as well as enhanced activity toward specific lectins.

Cited by 44 publications

References 52 publications

Synthesis of well-defined catechol polymers for surface functionalization of magnetic nanoparticles

Synthesis of well-defined catechol polymers for surface functionalization of magnetic nanoparticles

Anisotropic polydopamine capsules with an ellipsoidal shape that can tolerate harsh conditions: efficient adsorbents for organic dyes and precursors for ellipsoidal hollow carbon particles

One‐Pot, Multicomponent Strategy for Designing Lymphoseek‐Inspired Hetero‐Glycoadjuvant@AuNPs

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