Glycomyces amatae sp. nov., isolated from a yellow-ringed grass moth (Amata sperbius)

Abstract

A novel mycelium-forming actinomycete strain, designated A-F 0318T, was isolated from a yellow-ringed grass moth (Amata sperbius) collected from Phitsanulok Province, Thailand. Long chains of non-motile cylindrical spores with a smooth surface developed on aerial mycelia. The polyphasic taxonomic study suggested that strain A-F 0318T belonged to the genus Glycomyces . The 16S rRNA gene sequence analysis indicated that strain A-F 0318T was closely related to Glycomyces harbinensis LL-DO5139T with 97.94 % sequence similarity. The average nucleotide identity (ANI) based on blast, ANI based on the MUMmer algorithm and average amino acid identity values of strain A-F 0318T with G. harbinensis LL-DO5139T were 86.9, 89.1 and 84.24 %, respectively. The digital DNA–DNA hybridization value between A-F 0318T and its closest relative, G. harbinensis LL-DO5139T was 33.8 %. The digital G+C content of the genomic DNA was 71.7 mol%. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, xylose, glucose and galactose. The predominant menaquinone was MK-10(H4). The predominant fatty acids were iso-C16 : 0, anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 1 G. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unknown phosphoglycolipids and one unknown phospholipid. Based on comparative analysis of genotypic, phenotypic and chemotaxonomic data, the novel actinomycete strain A-F 0318T (=TBRC 13612T=NBRC 115417T) represents the type strain of a novel species, for which the name Glycomyces amatae sp. nov. is proposed.

Commensalibacter nepenthis sp. nov. and Commensalibacter oyaizuii sp. nov., from fluid in a Nepenthes pitcher cup and a butterfly (Junonia lemonias) in Thailand

“…It was then immersed in a solution of 10 % NaHCO 3 for a period of 10 min. Finally, the whole body, including wings, was ground according to the previously described method [21,22]. The treated samples were 10-fold serially diluted in normal saline (0.85 % (w/v) NaCl) as appropriate.…”