Glycomics in tears: seeking for new biomarkers for ocular allergy diagnosis

Fauquert, Jean‐Luc; Kowalski, Marek L.

doi:10.1111/all.14846

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…A proteómica e glicómica do fluido lacrimal têm vindo a ser estudadas como potenciais biomarcadores que permitam diferenciar as várias entidades dentro do espetro da alergia ocular. Alguns dos estudos já realizados demonstraram diferenças no perfil proteómico que poderão também estar associadas à gravidade da doença (36)(37)(38).…”

Section: Meios Complementares De Diagnósticounclassified

Alergia ocular: Da abordagem clínica ao tratamento

Mota,

Vasconcelos,

Moreira

et al. 2024

rpia

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A alergia ocular engloba um espetro de patologias mediadas por mecanismos de hipersensibilidade imunológica e que afetam a superfície ocular. Os sintomas e sinais oculares como o prurido ou hiperemia conjuntival são muitas vezes subvalorizados e subtratados apesar do seu impacto na qualidade de vida. As diferentes entidades englobadas na alergia ocular distinguem-se pelo seu mecanismo fisiopatológico e apresentação clínica. Para o seu diagnóstico, a história clínica e o exame objetivo são fundamentais, com eventual comprovação da existência de sensibilização a alergénios, recorrendo a testes in vivo e/ou in vitro ou através de provas de provocação. O tratamento deve ser guiado pela gravidade clínica, inclui medidas não farmacológicas e farmacológicas e, em casos selecionados, imunoterapia com alergénios. O presente artigo pretende perspetivar a atual abordagem multidisciplinar do doente com alergia ocular na prática clínica, no que concerne à sua fisiopatologia, classificação, diagnóstico e opções terapêuticas.

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Section: Meios Complementares De Diagnósticounclassified

Alergia ocular: Da abordagem clínica ao tratamento

Mota,

Vasconcelos,

Moreira

et al. 2024

rpia

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“…Proteomic analysis in ocular allergy favours tear collection methods such as microcapillary flow that minimise external sources of itchiness and irritation on the ocular surface. Microcapillary tear collection is simple, does not introduce irritation as a confounding variable, and permits extraction of good quality protein samples (up to 10 μg/μL) [ 26 , 27 , 28 ]. As shown in Figure 2 , in this technique a glass microcapillary tube is placed on the outer corner of the eye, collecting tears normally residing in the inferior fornix via capillary action.…”

Section: Current Tear Collection and Biomarker Analysis Techniques In...mentioning

confidence: 99%

A Review of Emerging Tear Proteomics Research on the Ocular Surface in Ocular Allergy

Aydin

Dhar

Gokhale

et al. 2022

Biology

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Ocular allergy is an immunoglobulin E-mediated Type I hypersensitivity reaction localised to the ocular surface and surrounding tissues. Primary signs and symptoms of ocular allergy include itching, redness, irritation and inflammation. Eye-rubbing caused by itching has been shown to alter ocular surface protein concentrations in conditions linked to ocular allergy such as keratoconus. In keratoconus, the cornea begins to thin and sag over time, leading to progressive vision loss and blindness in severe conditions. Due to the high incidence of ocular allergy sufferers rubbing their eyes in response to symptoms of itching, the protein landscape of the ocular surface may be significantly altered. Differential protein expression caused by long-term inflammation and eye-rubbing may lead to subsequent changes in ocular surface structure and function over time. This review aims to summarise and explore the findings of current ocular allergy proteome research conducted using techniques such as gel electrophoresis, mass spectrometry and lab-on-a-chip proteomics. Proteins of interest for this review include differentially expressed immunoglobulins, mucins, functional proteins, enzymes and proteins with previously uncharacterised roles in ocular allergy. Additionally, potential applications of this research are addressed in terms of diagnostics, drug development and future research prospects.

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Glycomics in tears: seeking for new biomarkers for ocular allergy diagnosis

Cited by 2 publications

References 9 publications

Alergia ocular: Da abordagem clínica ao tratamento

Alergia ocular: Da abordagem clínica ao tratamento

A Review of Emerging Tear Proteomics Research on the Ocular Surface in Ocular Allergy

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