Glycolytic enzyme inhibitory and antiglycation potential of rutin

Dubey, Shagun; Ganeshpurkar, Aditya; Ganeshpurkar, Ankit; Bansal, Divya; Dubey, Nazneen

doi:10.1016/j.fjps.2017.05.005

Cited by 42 publications

(35 citation statements)

References 26 publications

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“…Under our experimental conditions, rutin significantly inhibited α-glucosidase enzyme, with a potency similar to those of acarbose and PGE, as confirmed by the IC 50 values (Table 3). Our results agree with the literature, which highlights rutin to possess glycolytic enzyme inhibitory and antiglycation properties [47,48]. It also exhibited an acarbose-like and specific inhibitory effect on maltase activity in the intestine, and reduced glycemia when administered before glucose overload [49].…”

Section: Resultssupporting

confidence: 92%

Hypoglycemic, Antiglycation, and Cytoprotective Properties of a Phenol-Rich Extract From Waste Peel of Punica granatum L. var. Dente di Cavallo DC2

et al. 2019

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Pomegranate peel is a natural source of phenolics, claimed to possess healing properties, among which are antioxidant and antidiabetic. In the present study, an ethyl acetate extract, obtained by Soxhlet from the peel of Dente di Cavallo DC2 pomegranate (PGE) and characterized to contain 4% w/w of ellagic acid, has been evaluated for its hypoglycemic, antiglycation, and antioxidative cytoprotective properties, in order to provide possible evidence for future nutraceutical applications. The α-amylase and α-glucosidase enzyme inhibition, interference with advanced glycation end-products (AGE) formation, and metal chelating abilities were studied. Moreover, the possible antioxidant cytoprotective properties of PGE under hyperglycemic conditions were assayed. Phenolic profile of the extract was characterized by integrated chromatographic and spectrophotometric methods. PGE resulted able to strongly inhibit the tested enzymes, especially α-glucosidase, and exerted chelating and antiglycation properties. Also, it counteracted the intracellular oxidative stress under hyperglycemic conditions, by reducing the levels of reactive oxygen species and total glutathione. Among the identified phenolics, rutin was the most abundant flavonoid (about 4 % w/w). Present results suggest PGE to be a possible remedy for hyperglycemia management and encourage further studies to exploit its promising properties.

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Section: Resultssupporting

confidence: 92%

Hypoglycemic, Antiglycation, and Cytoprotective Properties of a Phenol-Rich Extract From Waste Peel of Punica granatum L. var. Dente di Cavallo DC2

et al. 2019

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“…Under our experimental conditions, rutin significantly inhibited α-glucosidase enzyme, being the potency similar to those produced by acarbose and PGE, as confirmed by the IC50 values (Table 3). Our results agree with literature, which highlighted rutin to possess glycolytic enzyme inhibitory and antiglycation potential properties [36][37]. It also exhibited an acarbose-like and specific inhibitory effect on maltase activity in the intestine, and reduced glycemia when administered before glucose overload [38].…”

Section: Metabolic Enzyme Inhibitionsupporting

confidence: 92%

Hypoglycemic, Antiglycation and Cytoprotective Properties of A Phenol Rich Extract from Waste Peel of <i>Punica Granatum</i> L. Var. Dente Di Cavallo DC2

Sotto¹,

Locatelli²,

Macone³

et al. 2019

Preprint

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Pomegranate peel is a natural source of phenolics, claimed to possess healing properties, among which antioxidant and antidiabetic. In line with this evidence, the ethyl acetate PGE extract, obtained by Soxhlet from the peel of Dente di cavallo DC2 variety and characterized by a 4% amount of ellagic acid, has been studied for its hypoglycemic, antiglycation and antioxidative cytoprotective properties, in order to support a possible further nutraceutical interest. The α-amylase and α-glucosidase enzyme inhibition, interference with advanced glycation end-products (AGE) formation and metal chelating abilities were evaluated as hypoglycemic mechanisms. Also, considering that oxidative stress is associated with hyperglycemia complications, PGE antioxidant cytoprotective properties under hyperglycemic conditions were assayed. Phenolic profile was characterized by integrated chromatographic and spectrophotometric methods. Under our experimental conditions, PGE strongly inhibited the tested enzymes, especially α-glucosidase, and exerted chelating and antiglycation properties. Also, it reduced both ROS and GSH levels under hyperglycemic conditions, thus suggesting its ability to support cell functions by counteracting intracellular oxidative stress. Along with ellagic acid, rutin was the major identified flavonoid (about 4 %) of PGE. Present results suggest PGE to be a possible remedy for hyperglycemia management and encourage further studies to exploit its promising properties.

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“…The a-glucosidase inhibition of the sample extract and acarbose were measured according to the assay described by (Dubey et al, 2017), with modi ications. The α-glucosidase (0.5 mg) dissolved in 0.1 M phosphate buffer (pH 7.0) containing bovine serum albumin (100 mg).…”

Section: A-glucosidase Inhibition Assaymentioning

confidence: 99%

Kinetics of α -glucosidase inhibitory activity and phytochemical analysis of Piper crocatum Ruiz & Pav. leaves ethanol extract

Alfarabi¹,

Bintang²,

Suryani³

et al. 2020

ijrps

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α-Glucosidase is an enzymes group that playing essential roles in the digestion of polysaccharide. Inhibitor of a-glucosidase can decrease polysaccharide digestion rate and therefore plays a significant function in preventing the development of diabetes (type 2). Piper crocatum Ruiz & Pav. is an essential herb applied traditionally in Indonesia to treat diabetes mellitus. This work evaluated the a-glucosidase inhibitory activity of P. crocatum leaves ethanol extract. Phytochemical component of the extract was also analyzed. The α-glucosidase inhibitory activity of the P. crocatum leaves ethanol extract was examined by reacting its different concentrations with α-glucosidase and p-nitrophenyl glucopyranoside. Kinetics of the α-glucosidase inhibition was determined using a Lineweaver-Burke plot. Phytochemical in the extract was determined using GC-MS. Ethanol extract of P. crocatum leaves exhibited moderate α-glucosidase inhibitory activity compared with acarbose. Phytochemical analyses showed the presence of stilbene, linolenic acid, phenol, phytosteroid, and α-tocopherol. The competitive action of P. crocatum leaves ethanol extract is due to its inhibitory effects on α-glucosidase. The stilbene and phenol compounds indicated responsible for anti-diabetic activity from P. crocatum leaves ethanol extract.

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Glycolytic enzyme inhibitory and antiglycation potential of rutin

Cited by 42 publications

References 26 publications

Hypoglycemic, Antiglycation, and Cytoprotective Properties of a Phenol-Rich Extract From Waste Peel of Punica granatum L. var. Dente di Cavallo DC2

Hypoglycemic, Antiglycation, and Cytoprotective Properties of a Phenol-Rich Extract From Waste Peel of Punica granatum L. var. Dente di Cavallo DC2

Hypoglycemic, Antiglycation and Cytoprotective Properties of A Phenol Rich Extract from Waste Peel of <i>Punica Granatum</i> L. Var. Dente Di Cavallo DC2

Kinetics of α -glucosidase inhibitory activity and phytochemical analysis of Piper crocatum Ruiz & Pav. leaves ethanol extract

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