Kinetics of α -glucosidase inhibitory activity and phytochemical analysis of Piper crocatum Ruiz & Pav. leaves ethanol extract

Abstract

α-Glucosidase is an enzymes group that playing essential roles in the digestion of polysaccharide. Inhibitor of a-glucosidase can decrease polysaccharide digestion rate and therefore plays a significant function in preventing the development of diabetes (type 2). Piper crocatum Ruiz & Pav. is an essential herb applied traditionally in Indonesia to treat diabetes mellitus. This work evaluated the a-glucosidase inhibitory activity of P. crocatum leaves ethanol extract. Phytochemical component of the extract was also analyzed. The α-glucosidase inhibitory activity of the P. crocatum leaves ethanol extract was examined by reacting its different concentrations with α-glucosidase and p-nitrophenyl glucopyranoside. Kinetics of the α-glucosidase inhibition was determined using a Lineweaver-Burke plot. Phytochemical in the extract was determined using GC-MS. Ethanol extract of P. crocatum leaves exhibited moderate α-glucosidase inhibitory activity compared with acarbose. Phytochemical analyses showed the presence of stilbene, linolenic acid, phenol, phytosteroid, and α-tocopherol. The competitive action of P. crocatum leaves ethanol extract is due to its inhibitory effects on α-glucosidase. The stilbene and phenol compounds indicated responsible for anti-diabetic activity from P. crocatum leaves ethanol extract.

Blood Glucose Level, Langerhans Pancreas and Lipid Profile of Diabetic Rats After Administration of Red Betel, Ginger and Cinnamon Combination Extract

“…Antihyperglycemic activity in vitro showed that ethyl acetate fraction of the red betel has an IC 50 value of 743.80 μg/mL with a competitive inhibitory mechanism to the alpha(α)-glucosidase ( Weni 2018 ). In addition, red betel ethanol extract exhibited a competitive inhibition for α-glucosidase, similar to acarbose on α-glucosidase inhibition ( Alfarabi et al . 2021 ).…”

“…Stilbene, phenol, linolenic acid, phytosteroid and α-tocopherol are bioactive compounds contained in the ethanol extract of red betel leaves. Stilbene and phenol have α-glucosidase inhibitory activity ( Alfarabi et al . 2021 ).…”