Glycolaldehyde impairs neutrophil biochemical parameters by an oxidative and calcium-dependent mechanism—Protective role of antioxidants astaxanthin and vitamin C

Guerra, Beatriz Alves; Bolin, Anaysa Paola; Morandi, Ana Carolina; Otton, Rosemari

doi:10.1016/j.diabres.2012.07.004

Cited by 12 publications

(9 citation statements)

References 66 publications

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“…When these cycles are overwhelmed, other cellular proteins may be irreversibly oxidised. It has been shown that antioxidant treatments will enhance the neutrophil redox state and the GSH/GSSG ratio [41]. …”

Section: Discussionmentioning

confidence: 99%

Sulforaphane Restores Cellular Glutathione Levels and Reduces Chronic Periodontitis Neutrophil Hyperactivity In Vitro

et al. 2013

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The production of high levels of reactive oxygen species by neutrophils is associated with the local and systemic destructive phenotype found in the chronic inflammatory disease periodontitis. In the present study, we investigated the ability of sulforaphane (SFN) to restore cellular glutathione levels and reduce the hyperactivity of circulating neutrophils associated with chronic periodontitis. Using differentiated HL60 cells as a neutrophil model, here we show that generation of extracellular O2 . - by the nicotinamide adenine dinucleotide (NADPH) oxidase complex is increased by intracellular glutathione depletion. This may be attributed to the upregulation of thiol regulated acid sphingomyelinase driven lipid raft formation. Intracellular glutathione was also lower in primary neutrophils from periodontitis patients and, consistent with our previous findings, patients neutrophils were hyper-reactive to stimuli. The activity of nuclear factor erythroid-2-related factor 2 (Nrf2), a master regulator of the antioxidant response, is impaired in circulating neutrophils from chronic periodontitis patients. Although patients’ neutrophils exhibit a low reduced glutathione (GSH)/oxidised glutathione (GSSG) ratio and a higher total Nrf2 level, the DNA-binding activity of nuclear Nrf2 remained unchanged relative to healthy controls and had reduced expression of glutamate cysteine ligase catalytic (GCLC), and modifier (GCLM) subunit mRNAs, compared to periodontally healthy subjects neutrophils. Pre-treatment with SFN increased expression of GCLC and GCM, improved intracellular GSH/GSSG ratios and reduced agonist-activated extracellular O2 . - production in both dHL60 and primary neutrophils from patients with periodontitis and controls. These findings suggest that a deficiency in Nrf2-dependent pathways may underpin susceptibility to hyper-reactivity in circulating primary neutrophils during chronic periodontitis.

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Section: Discussionmentioning

confidence: 99%

Sulforaphane Restores Cellular Glutathione Levels and Reduces Chronic Periodontitis Neutrophil Hyperactivity In Vitro

et al. 2013

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“…The maximum activity of this 10 enzyme was previously described by (Guerra et al, 2012)and was based on the conversion of NADP + in NADPH in the presence of glucose-6-phosphate.…”

Section: Glucose-6-phosphate Dehydrogenase (G6pdh) Activitymentioning

confidence: 99%

“…Changes in cytosolic Ca 2+ levels were monitored by fluorescence using the calciumsensitive probe Fura 2-AM (Guerra et al, 2012). The loading period for 5 µM of Fura 2-AM was 1 h at 37 ºC in 110 6 cells/well in Tyrode's solution.…”

Section: Intracellular Calcium Concentrationmentioning

confidence: 99%

Green tea catechins alone or in combination alter functional parameters of human neutrophils via suppressing the activation of TLR-4/NFκB p65 signal pathway

Marinovic

Morandi

Otton

2015

Toxicology in Vitro

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“…The maximum activity of this enzyme was described by Guerra and Otton [31] and was based on the conversion of NADP+ into NADPH in the presence of glucose-6-phosphate.…”

Section: Hexokinase and Glucose-6-phosphate Dehydrogenase (G6pdh) Actmentioning

confidence: 99%

“…Cells totalling 100 per plate were counted and multiplied by 1 (one or two particles), 2 (three or four particles) or 3 (more than four particles phagocytosed), according to a score criterion established previously by our group. The index of phagocytic activity was calculated by adding the points obtained per rat [31].…”

Section: Phagocytic Capacitymentioning

confidence: 99%

Green tea polyphenol extract in vivo attenuates inflammatory features of neutrophils from obese rats

et al. 2015

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Glycolaldehyde impairs neutrophil biochemical parameters by an oxidative and calcium-dependent mechanism—Protective role of antioxidants astaxanthin and vitamin C

Cited by 12 publications

References 66 publications

Sulforaphane Restores Cellular Glutathione Levels and Reduces Chronic Periodontitis Neutrophil Hyperactivity In Vitro

Sulforaphane Restores Cellular Glutathione Levels and Reduces Chronic Periodontitis Neutrophil Hyperactivity In Vitro

Green tea catechins alone or in combination alter functional parameters of human neutrophils via suppressing the activation of TLR-4/NFκB p65 signal pathway

Green tea polyphenol extract in vivo attenuates inflammatory features of neutrophils from obese rats

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