Glycogen phosphorylase b and phosphorylase kinase binding to glycogen under molecular crowding conditions. Inhibitory effect of FAD

Chebotareva, Natalia A.; Merem’yanin, A. V.; Makeeva, Valentina F.; Eronina, Tatiana B.; Bi, Kurganov

doi:10.1134/s0006297909050125

Cited by 7 publications

(5 citation statements)

References 40 publications

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“…Such large particles display very broad/not detectable signals for the core, and only sugar and aliphatic chains that are mobile tails can be detected. The calculated R h values are similar to those found for other carbohydrate-containing polymers like cellulose fibers [31], amylose [32], glycogen [33] and amylopectins [34]. Dinadayala and coworkers [35] reported for rabbit glycogen the values of the hydrodynamic radius and molecular mass of about 23 nm and 7 × 10 6 Da, respectively.…”

Section: Hydrodinamic Behaviorsupporting

confidence: 83%

Chemical characterization and surface properties of a new bioemulsifier produced by Pedobacter sp. strain MCC-Z

Beltrani¹,

Chiavarini²,

Cicero³

et al. 2015

International Journal of Biological Macromolecules

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Section: Hydrodinamic Behaviorsupporting

confidence: 83%

Chemical characterization and surface properties of a new bioemulsifier produced by Pedobacter sp. strain MCC-Z

Beltrani¹,

Chiavarini²,

Cicero³

et al. 2015

International Journal of Biological Macromolecules

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“…Molecular crowding noticeably influences interactions between macromolecules as well as the rate and equilibrium of intracellular biochemical processes 14–17, 24, 25. Analyzing the effect of crowding on phosphorylase b (Ph b ) and PhK, we found that crowding affects many important reactions of key enzymes of glycogenolysis such as association, conformational transitions, interaction with allosteric ligands, denaturation, and aggregation 10, 12, 13, 18–20, 24. Many effects of crowding seem to be a result of protein association and aggregation.…”

Section: Introductionmentioning

confidence: 94%

“…Crowding conditions in the cells are also induced by high concentrations of osmolytes,17 which are often used as space‐filling species in experiments that model crowding conditions in vitro. A number of investigations have been devoted to the study of the effect of trimethylamine N ‐oxide (TMAO) on protein stability and formation of supramolecular structures 18–24. Molecular crowding noticeably influences interactions between macromolecules as well as the rate and equilibrium of intracellular biochemical processes 14–17, 24, 25.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Hsp27 with Native Phosphorylase Kinase under Crowding Conditions

Chebotareva

Makeeva

Bazhina

et al. 2010

Macromolecular Bioscience

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Interaction of the wild type (wt) heat shock protein Hsp27 and its three-dimensional (3D) mutant (mimicking phosphorylation at Ser15, 78, and 82) with rabbit skeletal muscle phosphorylase kinase (PhK) has been studied under crowding conditions modeled by addition of 1 M trimethylamine N-oxide (TMAO). According to the data of sedimentation velocity and dynamic light scattering, crowding provokes the formation of large-sized associates of both PhK and Hsp27. Under crowding conditions, small associates of PhK and Hsp27 interact with each other thus leading to dissociation of large homooligomers of each protein. Taking into account high concentrations of PhK in the cell, we speculate that native PhK might modulate the oligomeric state and chaperone-like activity of Hsp27.

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“…They protect proteins under stress conditions by stabilization of protein structure. The stabilizing effect is due to preferential exclusion of the osmolyte from the surface of the protein. , One of the osmolytes that are widely used as crowding agents in model experiments is TMAO. ,,,− It was shown in vivo that TMAO counteracts the damaging action of the excess of urea and salts and hydrostatic pressure on deep-water animals. − It should be noted that TMAO has a p K a value of 4.66. The crowding action of TMAO is studied usually in the pH range of 6.0–8.0, where TMAO is almost neutral (zwitterionic form).…”

mentioning

confidence: 99%

Does the Crowded Cell-like Environment Reduce the Chaperone-like Activity of α-Crystallin?

et al. 2011

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The effect of crowding on the chaperone-like activity of α-crystallin has been studied using aggregation of UV-irradiated glycogen phosphorylase b (Phb) from rabbit skeletal muscle as an aggregation test system. The merit of this test system is the possibility of testing agents that directly affect the stage of aggregation of the protein molecules. It was shown that the solution of Phb denatured by UV contained aggregates with a hydrodynamic radius of 10.4 nm. These aggregates are relatively stable at 20 °C; however, they reveal a tendency to stick further in the presence of crowding agents. The study of the effect of α-crystallin on the aggregation of UV-irradiated Phb in the presence of the crowding agents by dynamic light scattering at 37 °C showed that under crowding conditions the antiaggregation ability of α-crystallin was weakened. On the basis of the analytical ultracentrifugation, size-exclusion chromatography, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis data, the scheme of interaction of UV-irradiated Phb and α-crystallin has been proposed. It is assumed that chaperone-target protein complexes of two types are formed, namely, the complexes of dissociated forms of α-crystallin with a protein substrate and high-mass α-crystallin-denatured protein complexes. The complexes of the first type reveal a weak propensity to aggregate even under crowding conditions. The complexes of the second type are characterized by the lower rate of aggregation in comparison with that of original UV-irradiated Phb. However, crowding stimulates the rate of aggregation of these complexes, resulting in the above-mentioned decrease in the chaperone-like activity of α-crystallin.

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Glycogen phosphorylase b and phosphorylase kinase binding to glycogen under molecular crowding conditions. Inhibitory effect of FAD

Cited by 7 publications

References 40 publications

Chemical characterization and surface properties of a new bioemulsifier produced by Pedobacter sp. strain MCC-Z

Chemical characterization and surface properties of a new bioemulsifier produced by Pedobacter sp. strain MCC-Z

Interaction of Hsp27 with Native Phosphorylase Kinase under Crowding Conditions

Does the Crowded Cell-like Environment Reduce the Chaperone-like Activity of α-Crystallin?

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