Glycoanalysis of the placental membrane glycoproteins throughout placental development

Robajac, Dragana; Masnikosa, Romana; Nemčovič, Marek; Križáková, Martina; Kluková, Ľudmila; Baráth, Péter; Katrlı́k, Jaroslav; Nedić, Olgica

doi:10.1016/j.mad.2019.111151

Cited by 9 publications

(4 citation statements)

References 51 publications

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“…Lectins are sugar-binding proteins that can specifically recognize glycans on glycoconjugates. Sambucus nigra lectin (SNA) and Maackia amurensis lectin (MAL) are commonly used to Cells 2020, 9, 273 7 of 18 recognize the α2,6-linked and α2,3-linked sialic acid residues, respectively [116][117][118], in lectin blot [119], lectin microarray [120][121][122], histochemistry [123], fluorescent image and flow cytometry [56,124]. Using lectin MAL-1, α2,3-linked sialic acids were found to promote gastric cancer cell metastasis [125].…”

Section: Sialic Acids Detection Through Bio-affinitymentioning

confidence: 99%

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Zhou

Yang

Guan

2020

Cells

120

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Sialic acids, a subset of nine carbon acidic sugars, often exist as the terminal sugars of glycans on either glycoproteins or glycolipids on the cell surface. Sialic acids play important roles in many physiological and pathological processes via carbohydrate-protein interactions, including cell–cell communication, bacterial and viral infections. In particular, hypersialylation in tumors, as well as their roles in tumor growth and metastasis, have been widely described. Recent studies have indicated that the aberrant sialylation is a vital way for tumor cells to escape immune surveillance and keep malignance. In this article, we outline the present state of knowledge on the metabolic pathway of human sialic acids, the function of hypersialylation in tumors, as well as the recent labeling and analytical techniques for sialic acids. It is expected to offer a brief introduction of sialic acid metabolism and provide advanced analytical strategies in sialic acid studies.

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Section: Sialic Acids Detection Through Bio-affinitymentioning

confidence: 99%

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Zhou

Yang

Guan

2020

Cells

120

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“…Hence, pre-treatment of the sample is required to successfully characterise the full glycome. Regarding the determination techniques, a broad range of analytical platforms can be applied for the profiling, characterisation and analysis of glycans, being the most widely used liquid chromatography coupled with fluorescence detection (LC-FLR) [ 9 , 48 , 97 , 100 ], lectin-based microarray [ 50 , 82 , 85 ], capillary electrophoresis (CE) [ 58 , 71 , 118 ], matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) [ 52 , 75 , 92 , 112 ], LC-MS [ 23 , 24 , 49 , 96 ], DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) [ 19 , 22 , 74 , 79 ], ion mobility (IM) and NMR [ 93 ]. Several reviews focus exclusively on describing the available techniques used for glycan analysis [ 129 , 130 , 131 ].…”

Section: Glycomics Techniquesmentioning

confidence: 99%

Glycosylation Biomarkers Associated with Age-Related Diseases and Current Methods for Glycan Analysis

Paton

Suárez

Herrero

et al. 2021

IJMS

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Ageing is a complex process which implies the accumulation of molecular, cellular and organ damage, leading to an increased vulnerability to disease. In Western societies, the increase in the elderly population, which is accompanied by ageing-associated pathologies such as cardiovascular and mental diseases, is becoming an increasing economic and social burden for governments. In order to prevent, treat and determine which subjects are more likely to develop these age-related diseases, predictive biomarkers are required. In this sense, some studies suggest that glycans have a potential role as disease biomarkers, as they modify the functions of proteins and take part in intra- and intercellular biological processes. As the glycome reflects the real-time status of these interactions, its characterisation can provide potential diagnostic and prognostic biomarkers for multifactorial diseases. This review gathers the alterations in protein glycosylation profiles that are associated with ageing and age-related diseases, such as cancer, type 2 diabetes mellitus, metabolic syndrome and several chronic inflammatory diseases. Furthermore, the review includes the available techniques for the determination and characterisation of glycans, such as liquid chromatography, electrophoresis, nuclear magnetic resonance and mass spectrometry.

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“…The authors of this study did not find any changes in the plasma glycome of ASD patients [28]. In our work, we applied combined glycan analysis using two different techniques, lectin-based glycoprotein microarray and matrix-assisted laser desorption/ionization time-of-flight MALDI-TOF MS methods [29,30], to study alterations in glycosylation in sera from ADHD patients and to search for possible glycan biomarkers of ADHD. Due to the need to improve the quality of ADHD treatment, there is still a strong demand for better and more complex diagnostics.…”

Section: Introductionmentioning

confidence: 88%

Alterations in the Glycan Composition of Serum Glycoproteins in Attention-Deficit Hyperactivity Disorder

Kianičková,

Pažitná,

Kundalia

et al. 2023

IJMS

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Changes in protein glycosylation are associated with most biological processes, and the importance of glycomic analysis in the research of disorders is constantly increasing, including in the neurodevelopmental field. We glycoprofiled sera in 10 children with attention-deficit hyperactivity disorder (ADHD) and 10 matching healthy controls for 3 types of samples: whole serum, sera after depletion of abundant proteins (albumin and IgG), and isolated IgG. The analytical methods used were a lectin-based glycoprotein microarray enabling high-throughput glycan analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) as a standard method for the identification of glycan structures. For microarray analysis, the samples printed on microarray slides were incubated with biotinylated lectins and detected using the fluorescent conjugate of streptavidin by a microarray scanner. In the ADHD patient samples, we found increased antennary fucosylation, decreased di-/triantennary N-glycans with bisecting N-acetylglucosamine (GlcNAc), and decreased α2-3 sialylation. The results obtained by both independent methods were consistent. The study’s sample size and design do not allow far-reaching conclusions to be drawn. In any case, there is a strong demand for a better and more comprehensive diagnosis of ADHD, and the obtained results emphasize that the presented approach brings new horizons to studying functional associations of glycan alterations in ADHD.

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Glycoanalysis of the placental membrane glycoproteins throughout placental development

Cited by 9 publications

References 51 publications

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Glycosylation Biomarkers Associated with Age-Related Diseases and Current Methods for Glycan Analysis

Alterations in the Glycan Composition of Serum Glycoproteins in Attention-Deficit Hyperactivity Disorder

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