Glycerolation and Thawing Effects on Bull Spermatozoa Frozen in Detergent-Treated Egg Yolk and Whole Egg Extenders

Arriola, J.; Foote, R.H.

doi:10.3168/jds.s0022-0302(87)80194-7

Cited by 57 publications

(52 citation statements)

References 23 publications

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“…It has been suggested that the effect of SLS might be exerted by modifying the structure of egg yolk lipoproteins in the extracellular medium [4]. Kato et al [9] employed a freezing extender of boar semen containing SLS (1.2 to 1.6 mg/ml) instead of OEP and reported that the percentages of normal acrosome caps and progressively motile sperm after thawing with addition of SLS were similar to those of OEP.…”

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confidence: 99%

Usefulness of Addition of Orvus ES Paste and Sodium Lauryl Sulfate to Frozen Feline Semen

Mizutani

Sumigama²,

Nagakubo

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. It has been shown that addition of the surfactant Orvus ES paste (OEP) and its main component sodium lauryl sulfate (SLS) to boar or dog semen before freezing improves post-thaw sperm motility and protects acrosome caps. In this study, we investigated the usefulness of the addition of OEP (0, 1, 2 and 4%) or SLS (0, 1, 2, 3 and 4 mg/ml) to cat ejaculates before freezing and their concentrations. Among the OEP addition groups, the 1% OEP group showed higher sperm motility than the other groups. Among the SLS addition groups, the 3 mg/ml SLS group showed slightly higher sperm motility and viability than the other groups. Comparison between the 1% OEP and 3 mg/ml SLS addition groups suggested a higher percentage of sperm with an acrosome cap in the 1% OEP group. The other sperm properties did not significantly differ between the 2 groups. These results indicate that addition of 1% OEP or 3 mg/ ml SLS is effective for freezing of cat ejaculated semen.KEY WORDS: acrosome cap, cat, frozen semen, orvus es paste, sodium lauryl sulfate.J. Vet. Med. Sci. 72(1): 23-27, 2010 In regard to artificial insemination (AI) with frozen feline semen, intravaginal [6,11,19] and intrauterine [6,18,19] inseminations with ejaculates have each been reported in three studies, and intravaginal [20] and intrauterine [15,20] inseminations with epididymal sperm have each been reported in one and two studies, respectively. In our 2003 year study using epididymal sperm [15], we added 7% (v/v) glycerol and 1% Orvus ES paste (OEP, also known as Equex STM paste, Nova Chemical Sales, Inc., Scituate, MA, U.S.A.) to semen as cryoprotective agents and achieved a conception rate of 27.3%. However, we did not evaluate the usefulness of addition of OEP for cryopreservation of feline semen or its optimal concentration. Addition of OEP along with glycerol for the cryopreservation of boar [12] and dog [16,17] semen has been shown to protect the acrosome caps of sperm, thereby increasing and maintaining post-thaw sperm motility for a long period of time. In 2004, Axnér et al. [5] investigated the usefulness of Equex STM paste for cryopreservation of feline epididymal sperm and showed that addition 0.5% (v/v) Equex STM paste led to greater protection of the acrosome caps of sperm after freeze-thawing than no addition, but sperm motility was lower (sperm survival was shorter) 4-6 hr after thawing than in the case of no addition. Our search of the literature revealed no studies in which addition of OEP for semen cryopreservation adversely affected post-thaw sperm motility. It remains unclear whether the results of the study by Axnér et al. [5] regarding cryopreservation of epididymal sperm apply to cryopreservation of cat ejaculates.OEP is known to contain mainly sodium lauryl sulfate (SLS), but data on its concentration and the remaining constituents have not been published. It has been suggested that the effect of SLS might be exerted by modifying the structure of egg yolk lipoproteins in the extracellular medium [4]. Kato et al. [9] employed ...

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mentioning

confidence: 99%

Usefulness of Addition of Orvus ES Paste and Sodium Lauryl Sulfate to Frozen Feline Semen

Mizutani

Sumigama²,

Nagakubo

et al. 2010

The Journal of Veterinary Medical Science

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“…The effectiveness of OEP in the cryopreservation of semen of different animal species has been reported by several authors (Arriola and Foote 1987, Aboagla and Terada 2004, Axner et al 2004, Niżański et al 2009). The findings of the current study showed that OEP, used at a concentration of 0.25%, gave comparable results to extender supplemented with 0.50% OEP, in terms of post-thaw sperm TMOT.…”

Section: Discussionmentioning

confidence: 99%

“…It was suggested that bull spermatozoa frozen in HEY-based extender supplemented with SDS were less susceptible to osmotic shock (Arriola and Foote 1987). Previous studies demonstrated that OEP improved post-thaw viability of boar spermatozoa only in the presence of egg yolk components, presumably by modifying the structure of egg yolk lipids, making them more easily accessible to spermatozoa, probably by allowing integrations of the lipids into the membrane bilayer (Pursel et al 1978, Pettit andBuhr 1998).…”

Section: Discussionmentioning

confidence: 99%

“…In this study 0.25% OEP was the optimal concentration in the LPFo-based extender to protect sperm motility apparatus after freezing-thawing. Several studies have reported about the toxic effects of OEP components on sperm function (Pursel et al 1978, Arriola and Foote 1987, Kato et al 1990, Niżański et al 2009). It is worth noting that the beneficial effect of OEP on post-thaw semen quality was time dependent (Peñ a and Linde-Forsberg 2000, Axnér et al 2004).…”

Section: Discussionmentioning

confidence: 99%

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Supplementation of different concentrations of Orvus Es Paste (OEP) to ostrich egg yolk lipoprotein extender improves post-thaw boar semen quality

Fraser¹,

Jasiewicz²,

Kordan³

2014

Polish Journal of Veterinary Sciences

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This study aimed to compare post-thaw quality of boar semen following freezing in an ostrich egg yolk lipoprotein (LPFo) extender supplemented with 0%, 0.25% and 0.50% Orvus Es Paste (OEP). Sperm assessments included total motility (TMOT), mitochondrial function (MF), plasma membrane integrity (PMI) and acrosome integrity (normal apical ridge, NAR). Considerable variations among boars and OEP treatments had a significant effect (P < 0.001) on post-thaw sperm characteristics. It was observed that post-thaw sperm characteristics were significantly compromised in semen samples frozen in the absence of OEP. By contrast, lactose-LPFo-glycerol extender supplemented with either 0.25% OEP or 0.50% OEP markedly enhanced post-thaw sperm characteristics. In all boars, there were no marked differences in post-thaw sperm TMOT between the freezing extenders supplemented with 0.25% and 0.50% OEP. However, a decline in the percentage of post-thaw motile spermatozoa was more pronounced in the extender supplemented with 0.50% OEP following a 120-min incubation period. Furthermore, the proportions of frozen-thawed spermatozoa with MF, PMI and NAR acrosomes varied significantly among the boars in the OEP-supplemented extenders. The findings of this study indicate that different OEP concentrations, in the presence of ostrich egg yolk lipoproteins, could have varying effects on post-thaw sperm survival.

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“…Sperm freezing procedures need to be optimized for species (2,3,38,39,51) and also for strains. Studies demonstrated that post-thaw sperm quality was highly variable between mouse strains (30,43).…”

Section: Discussionmentioning

confidence: 99%

Influence of extenders and cooling rates on epididymal sperm of Lewis rat strain

AGCA¹

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The objective of this study was to determine the appropriate cooling rates and extender for improving the freezability of epididymal sperm of Lewis rat strain. The epididymal sperm from Lewis rats were suspended in different freezing extenders which were HEPES buffered Tyrode's lactate (TL-HEPES), modified Kreb's Ringer bicarbonate (mKRB), 3% dehydrated skim milk (SM), Salamon's Tris-citrate (TRIS), and TES/Tris (TES). The extenders contained 20% (v/v) egg yolk (EY), 0.75% equex paste (EP) and 0.1 M raffinose or 0.1 M sucrose. The sperm samples were frozen by automated freezer, using various cooling rates (10,40, 70, and 100 °C/min). After thawing, sperm samples were examined for integrity of plasma and acrosomal integrity, and mitochondrial membrane potential (MMP). Type of extenders had significant effect on cryosurvival (p<0.05). The post-thaw highest motility (27.5%) was achieved for the sperm samples frozen in the presence of TES extender (p<0.05). As a result, although cooling rate did not significantly affect cryopreservation, the extenders affected post-thaw sperm parameters and TES considerably improved the freezability of epididymal Lewis rat sperm.Key words: Cooling rate, epididymal sperm, extenders, freezing, Lewis rat strain. Lewis ırkı rat sperması üzerine değişik sulandırıcı ve dondurma hızının etkisiÖzet: Çalışmanın amacı Lewis ırkı rat sperması için, uygun dondurma hızı ve sulandırıcılar tespit ederek spermanın dondurulabilirliğinin artırılmasıdır. Ratlardan alınan epididimal sperma HEPES buffered Tyrode's lactate (TL-HEPES), modified Kreb's Ringer bicarbonate (mKRB), %3 süt tozu (SM), Tris-citrate (TRIS) ve Tris/TES (TES) solüsyonları ile sulandırıldı. Sulandırıcılara 20% (v/v) yumurta sarısı, 0.75% Equex Paste ve 0.1 M rafinoz yada 0.1 M sükroz ilave edildi. Sperma örnekleri kontrollü dondurucu kullanılarak değişik soğutma hızında (10, 40, 70 ve 100 °C/dk) donduruldu. Dondurma sonrası spermalar plazma, akrozom ve mitokondri membran bütünlüğü yönünden değerlendirildi. Sulandırıcı tipinin dondurmada etkili olduğu saptandı (p<0.05). Dondurma-çözüm işlemi sonrası en yüksek motilite (%27.5) TES sulandırıcısından elde edildi (p<0.05). Sonuç olarak, dondurma hızının sperm dondurulmasına önemli bir etkisi tespit edilemese de, sulandırıcıların dondurma-çözüm sonrası sperm parametrelerini etkilediği ve TES'in epididimal Lewis rat sperması dondurulabilirliğini, önemli derecede geliştirdiği saptandı. Anahtar sözcükler: Dondurma, dondurma hızı, epididimal sperma, sulandırıcı, Lewis rat ırkı.

show abstract

Glycerolation and Thawing Effects on Bull Spermatozoa Frozen in Detergent-Treated Egg Yolk and Whole Egg Extenders

Cited by 57 publications

References 23 publications

Usefulness of Addition of Orvus ES Paste and Sodium Lauryl Sulfate to Frozen Feline Semen

Usefulness of Addition of Orvus ES Paste and Sodium Lauryl Sulfate to Frozen Feline Semen

Supplementation of different concentrations of Orvus Es Paste (OEP) to ostrich egg yolk lipoprotein extender improves post-thaw boar semen quality

Influence of extenders and cooling rates on epididymal sperm of Lewis rat strain

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