Supplementation of different concentrations of Orvus Es Paste (OEP) to ostrich egg yolk lipoprotein extender improves post-thaw boar semen quality

Abstract

This study aimed to compare post-thaw quality of boar semen following freezing in an ostrich egg yolk lipoprotein (LPFo) extender supplemented with 0%, 0.25% and 0.50% Orvus Es Paste (OEP). Sperm assessments included total motility (TMOT), mitochondrial function (MF), plasma membrane integrity (PMI) and acrosome integrity (normal apical ridge, NAR). Considerable variations among boars and OEP treatments had a significant effect (P < 0.001) on post-thaw sperm characteristics. It was observed that post-thaw sperm characteristics were significantly compromised in semen samples frozen in the absence of OEP. By contrast, lactose-LPFo-glycerol extender supplemented with either 0.25% OEP or 0.50% OEP markedly enhanced post-thaw sperm characteristics. In all boars, there were no marked differences in post-thaw sperm TMOT between the freezing extenders supplemented with 0.25% and 0.50% OEP. However, a decline in the percentage of post-thaw motile spermatozoa was more pronounced in the extender supplemented with 0.50% OEP following a 120-min incubation period. Furthermore, the proportions of frozen-thawed spermatozoa with MF, PMI and NAR acrosomes varied significantly among the boars in the OEP-supplemented extenders. The findings of this study indicate that different OEP concentrations, in the presence of ostrich egg yolk lipoproteins, could have varying effects on post-thaw sperm survival.

Preservation of boar semen: An update

“…The addition of Orvus ES Paste (OEP) to the freezing extender provides better protection of the plasma membrane, since its detergent properties allow a better interaction of the egg yolk proteins and the sperm membrane in boars; supplementation with 0.25% and 0.50% OEP to the freezing extender using ostrich egg yolk and glycerol enhances the seminal characteristics with respect to mitochondrial function, total motility, plasma membrane integrity and acrosomal integrity (Fraser, Jasiewicz, & Kordan, ).…”

Interaction among extenders, cryoprotectants and Orvus Es Paste supplementation and freezing rate for sperm cryopreservation in the dromedary camel

“…Orvus Es Paste (Equex: OEP) is a synthetic surfactant, the main component being sodium lauryl sulphate, that is often used in extenders for freezing boar (Bottini‐Luzardo et al., 2013; Fraser et al., 2014; Yi et al., 2002) and dog (Hori et al., 2006; Tsutsui et al., 2000) spermatozoa. It protects acrosome integrity, and the duration of post‐thaw sperm motility is prolonged.…”