Glycerol as a novel green mobile phase modifier for reversed phase liquid chromatography

Habib, Ahmed; Mabrouk, Mokhtar M.; Fekry, Marwa; Mansour, Fotouh R.

doi:10.1016/j.microc.2021.106587

Cited by 12 publications

(5 citation statements)

References 26 publications

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“…For instance, the increase of the column temperature of 10°C led to a 20% decrease in column back pressure. The research results regarding the influence of the glycerol viscosity on the terms from the Van Deemter equation showed that higher viscosity of the glycerol-based mobile phase led to a decrease of the eddy diffusion (A term) and a decrease of the longitudinal diffusion (B term) [49]. This could be the reason for the good chromatographic performance in terms of peak symmetry, column efficiency, and resolution.…”

Section: Glycerolmentioning

confidence: 99%

“…Glycerol is an organic solvent that is the most recently included in the development of green chromatographic methods. In 2021, glycerol was used for the separation of four antiviral medicines under reversed-phase chromatographic conditions [49]. The same group of authors used this solvent as a mobile phase modifier for the determination of ascorbic acid and glutathione in tablets [50].…”

Section: Glycerolmentioning

confidence: 99%

“…Due to the higher viscosity, it is recommended for glycerol to be premixed with the water phase, followed by sonication. This step will reduce the pump load of the LC system and will facilitate the mixing process [49,50]. In addition, the issue with higher viscosity could be overcome with the use of higher column temperature, because this parameter has a significant impact on the column back pressure.…”

Section: Glycerolmentioning

confidence: 99%

See 2 more Smart Citations

Green Strategies toward Eco-Friendly HPLC Methods in Pharma Analysis

Nakov¹,

Acevska²,

Brezovska³

et al. 2023

High Performance Liquid Chromatography - Recent Advances and Applications

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The global need for changing the processes in order to meet the green analytical chemistry (GAC) criteria is a great challenge for the pharmaceutical industry. High-performance liquid chromatography (HPLC), as one of the most frequently used techniques in various stages in the pharmaceutical industry, generates huge amounts of organic toxic waste. Therefore, the implementation of the GAC principles in pharma analysis is highly required. Although the number of published papers concerning green chromatography approaches is constantly increasing, the use of eco-friendly HPLC methods in the pharma industry has not been widely implemented. The reasons for this mainly include the need for adaptation of the conventional HPLC instruments, lack of time, lack of experience, or uncertainty of the analysts regarding fulfillment of the method criteria. In this chapter, an overview of green strategies that can be easily applied to conventional instruments for liquid chromatography (LC) in developing eco-friendly HPLC methods in pharma analysis is given. The aim is to emphasize that the green method development in pharma analysis can be easily accomplished and to encourage the analytical community in the pharmaceutical industry not only to develop but also to transfer the already established conventional HPLC methods into green ones.

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Section: Glycerolmentioning

confidence: 99%

Section: Glycerolmentioning

confidence: 99%

Section: Glycerolmentioning

confidence: 99%

See 1 more Smart Citation

Green Strategies toward Eco-Friendly HPLC Methods in Pharma Analysis

Nakov¹,

Acevska²,

Brezovska³

et al. 2023

High Performance Liquid Chromatography - Recent Advances and Applications

View full text Add to dashboard Cite

show abstract

“…Table S9 provides explanation of GAPI results. It should be noted that the method could have been greener if a green solvent was used instead of methanol as a mobile phase modifier such as ethanol, acetone and glycerol [29][30][31].…”

Section: Greenness Assessmentmentioning

confidence: 99%

Development of a stability indicating high-performance liquid chromatography method for determination of cenobamate: study of basic degradation kinetics

EL-Malla,

Mansour,

Elbastawissy

et al. 2024

BMC Chemistry

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This study presents a stability indicating high-performance liquid chromatography HPLC method for the determination of cenobamate (CNB) in presence of its main impurity (CNB H-impurity) and degradation products. The chromatographic separation was carried out on a Thermo BDS Hypersil-C18 column (150 × 4.6 mm; 5 μm) with a mobile phase consisting of a 50:50 (%v/v) ratio of methanol and purified water. The flow rate was maintained at 1.0 mL. min− 1. CNB was detected at 210 nm using a PDA detector. The column temperature was held at 40 °C.The retention time of the drug was found to be 3.2 min. Furthermore, the study investigates the degradation behavior of CNB under various stress conditions, including acidic, basic, oxidative, and light-induced degradation. The results indicate that CNB is particularly susceptible to basic degradation. Consequently, a comprehensive study of the basic degradation kinetics was conducted. The method was also successfully applied for the determination of CNB in its dosage form. The results also show that there is no co-elution from degradation products or excipients as indicated by the mass balance and peak purity values confirming the specificity of the proposed method and its applicability for routine analysis of CNB.

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“…There were different methods reported in literature for ganciclovir analysis such as electrochemical method [8-14], spectrophotometric method [15][16][17][18][19][20][21][22][23][24][25], Raman spectroscopic method [26-28] and chromatographic methods [29][30][31][32][33][34][35][36][37][38]. Chemical products can result in harmful hazards to human and environment as, flammability, mutagenicity, carcinogenicity, and atmospheric damage.…”

Section: Introductionmentioning

confidence: 99%

Simple green RP-UPLC method for the analysis of ganciclovir in its bulk form and pharmaceutical preparations

Danial

Gamal²,

Saad³

et al. 2022

Egypt. J. Chem.

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A green, simple, validated reversed-phase ultra -. performance liquid chromatographic method was developed for the analysis of ganciclovir in both bulk and two different dosage forms. The optimum separation was reached using Acclaim TM RSLC.120 C18…column..(2.1.x.100.mm,.2.2.µm) at 30 °C using the mobile phase composed of methanol (60%) and 20 mM phosphate buffer,. pH (4.00) 40%; at 0.5 mL/min. flow rate. The measurement took place at 253 nm using a photodiode array detector. The method was linear at concentrations ranging from 20.00 to 300.00 µg/mL. The limit of detection and the limit of quantification were 7.00.µg/mL and 20.00 µg/mL, respectively. The range for the percentage relative standard deviation of intra.-. day was 0.38 to 1.10 %, while for inter-day precisions was 0.56 to 2.08 %, respectively. The method was accurate, with percentage recovery ranging from 98.71 to 101.02 % and percentage relative standard…deviation ranging from 0.38 to 1.10 %. The method was favorably applied for determining of ganciclovir in bulk, pharmaceutical tablet, and gel preparation. The greenness of the method was assessed by the analytical. eco-scale system, and it was found to be eco-friendly.

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Glycerol as a novel green mobile phase modifier for reversed phase liquid chromatography

Cited by 12 publications

References 26 publications

Green Strategies toward Eco-Friendly HPLC Methods in Pharma Analysis

Green Strategies toward Eco-Friendly HPLC Methods in Pharma Analysis

Development of a stability indicating high-performance liquid chromatography method for determination of cenobamate: study of basic degradation kinetics

Simple green RP-UPLC method for the analysis of ganciclovir in its bulk form and pharmaceutical preparations

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