Glycation-induced inactivation and loss of antigenicity of catalase and superoxide dismutase

Zhang, Pengcheng; Harding, John J.

doi:10.1042/bj3280599

Cited by 229 publications

(127 citation statements)

References 35 publications

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“…37 All assays were carried out in triplicate. Activity is expressed relative to the control activity at each respective time interval, set at 100%.…”

Section: Catalase Assaymentioning

confidence: 99%

Decreased chaperone activity of α-crystallin in selenite cataract may result from selenite-induced aggregation

Yan

Harding

Hui

et al. 2003

Eye

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Purpose To investigate the role of chaperone activity of a-crystallin in selenite-induced cataract formation. Methods Selenite cataract was induced in Sprague-Dawley rats by five subcutaneous injections of sodium selenite over a 20-day period starting at 8-10 days postpartum. a-Crystallin was separated from the rat lenses by size-exclusion chromatography. Bovine a L -crystallin and b L -crystallin were isolated for studies in vitro, and for the chaperone assays. The protective effects of both a H -and a L -crystallin were measured spectrophotometrically in four different assay procedures including the thermally induced aggregation of catalase and b L -crystallin, and the fructation-and heat-induced inactivation of catalase. The bovine a L -crystallin was incubated with different concentrations of sodium selenite for 72 h and then its chaperone activity against heat-induced b L -crystallin aggregation was assayed. The aggregation of selenite-treated a L -crystallin was analysed by molecular sieve high-performance liquid chromatography (HPLC). Results The protection of a H -crystallin was less than that of a L -crystallin in both normal and cataractous lenses. The chaperone activities of both a H -and a L -crystallin in selenite cataract were decreased compared with normal lenses. The protection provided by both a H -crystallin and a L -crystallin against the thermal aggregation of catalase was much greater than their protection against thermally and chemically induced inactivation. HPLC analysis demonstrated aggregation of a-crystallin by sodium selenite after 24 h incubation in a dose-dependent fashion. ConclusionThe chaperone activity of a-crystallin presented parallel patterns of activity with different methods, further supporting the view that the different assays measure essentially the same property. The decreased chaperone activity of a-crystallin in selenite cataract may result from seleniteinduced aggregation.

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“…37 All assays were carried out in triplicate. Activity is expressed relative to the control activity at each respective time interval, set at 100%.…”

Section: Catalase Assaymentioning

confidence: 99%

Decreased chaperone activity of α-crystallin in selenite cataract may result from selenite-induced aggregation

Yan

Harding

Hui

et al. 2003

Eye

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“…The decrease in CAT activity could have resulted from in activation by glycation of enzyme [20]. In the present study, extract treated groups showed a significant increase in the hepatic and renal SOD and CAT activities of the diabetic rats.…”

Section: Discussionmentioning

confidence: 50%

Effect of <i>Astragalus membranaceus</i> (Fisch) Bunge extract on streptozocin-induced diabetic in rats

Huo¹,

Yuan²,

Liang³

et al. 2016

Trop. J. Pharm Res

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(109.438 ± 3.52, p < 0.05) and increased insulin level (13.96 ± 0.74, p < 0.05). Furthermore, it significantly reduced biochemical parameters (serum creatinine, 0.86 ± 0.29, p < 0.05) and serum urea (45.14 ± 1.79, p < 0.05). The treatment also resulted in significant increase in GSH (49.21 ± 2.59, p < 0.05), GPx (11.96 ± 1.16, p < 0.05), SOD (14.13 ± 0.49, p < 0.05), CAT (83.25 ± 3.14, p < 0.05)

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“…CAT is a hemeprotein which catalyses the reduction of H 2 O 2 and protects the tissues from highly reactive hydroxyl radicals. The decreased activity of CAT during diabetes may be due to excess production of H 2 O 2 (Yan and Harding, 1997). Drug treatment resulted in a significant elevation of intestinal CAT in diabetic rats proving that AGLE can reduce glycation of enzymes or ROS and improve the antioxidant status.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of Asystasia gangetica reduced oxidative damage in the small intestine of streptozotocin-induced diabetic rats

Kumar¹,

Umamaheswari²,

Sivashanmugam³

et al. 2009

Oriental Pharmacy and Experimental Medicine

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SUMMARYOxidative stress plays an important role in the pathogenesis of various diabetic complications and small intestine is vulnerable to damage resulting in morphological and functional changes. In this study, the effects of Asystasia gangetica leaf extract (AGLE) on oxidative stress status in small intestine of diabetic rats were examined. The leaves of Asystasia gangetica was extracted with 70% ethanol. Oral administration of AGLE once daily (100 mg/kg and 200 mg/kg b.w.) for 28 days to diabetic rats significantly (P < 0.05) increased antioxidant levels of catalase, superoxide dismutase, glutathione peroxidase, glutathione, GSSH, carbohydrate metabolizing enzyme, glucose-6-phosphate dehydrogenase. The increased levels of protein carbonyl content, lipid peroxidation and xanthine oxidase/xanthine dehydrogenase in diabetic rats were reverted back to near normal levels on treatment with AGLE. Both doses of AGLE offered significant activity (P < 0.01) against oxidative damage and were comparable with standard, glibenclamide. The results revealed the occurrence of oxidative stress in small intestine during diabetes and suggest the potential of AGLE as an antioxidant in protecting the tissue defense system against oxidative damage in streptozotocin-induced diabetes.

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Glycation-induced inactivation and loss of antigenicity of catalase and superoxide dismutase

Cited by 229 publications

References 35 publications

Decreased chaperone activity of α-crystallin in selenite cataract may result from selenite-induced aggregation

Decreased chaperone activity of α-crystallin in selenite cataract may result from selenite-induced aggregation

Effect of <i>Astragalus membranaceus</i> (Fisch) Bunge extract on streptozocin-induced diabetic in rats

Protective effect of Asystasia gangetica reduced oxidative damage in the small intestine of streptozotocin-induced diabetic rats

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