Glycans as biochemical markers of human endometrial secretory differentiation

Aplin, John D.

doi:10.1530/jrf.0.0920525

Cited by 55 publications

(33 citation statements)

References 37 publications

(58 reference statements)

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“…By use of marker-coupled lectins, carbohydrate residues that might mediate interactions of cells with other cells, the extracellular matrix, hormones, and growth factors can be identified in situ (Raedler and Schreiber, 1988). These techniques have been used to identify changes in cell surface glycoconjugates during the course of development of a number of organs, including the uterus, in a variety of animals (Aplin, 1991;Aoki et al, 1989;Bartol et al, 1988b;Lee and Damjanov. 1985;Bernfield et al, 1984;Wu et al.…”

Section: Discussionmentioning

confidence: 99%

“…Lectins used, their source, and corresponding carbohydrate specificities are listed in Table 1. The lectins employed in this study were chosen on the basis of reports that the carbohydrate moieties which they specify may be involved in development and differentiation of uterine tissues (Aplin, 1991; Raedler and Schreiber, 1988; Rober and Holtz, 1988; Bartol et al, 1988b).…”

Section: Methodsmentioning

confidence: 99%

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Lectin binding sites as markers of neonatal porcine uterine development.

Spencer

Wiley²,

Bartol³

1992

J Histochem Cytochem.

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To identify lectin binding sites and to determine if lectin binding patterns change with age in developing neonatal porcine uterine tissues, gilts (n = 3/day) were hysterectomized on Day 0 (birth), 7, 14,28,42, or 56. Lectin binding was visualized in Bouin's-fued utetine tissues with seven biotinylated lectins (ConA, DBA, PNA, RCA-I, SBA, UEA-I, and WGA) and avidin-peroxidase staining procedures. Leain specificities were demonstrated by pre-incubating lectins with appropriate inhibitory sugars (0.2 M). Staining intensity was evaluated visually (absent, weak, moderate, or strong) for three endometrial tissues; luminal epithelium, glandular epithelium, and stroma. Staining intensities for DBA, PNA, SBA, and WGA were not affected by neonatal age. Staining with these lectins was greater in uterine epithelium (moderate or strong) than in stroma (weak). In contrast, binding patterns for ConA, UEA-I, and RCA-I were affected by neonatal age. Strong epithelial staining associated with ConA

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Lectin binding sites as markers of neonatal porcine uterine development.

Spencer

Wiley²,

Bartol³

1992

J Histochem Cytochem.

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“…40 For example, pancreatic MUC1 is larger and more glycosylated than that from breast or endometrium. 41,42 While endometrial MUC1 glycotopes have been the subject of many studies, 19,20,22,43 data regarding carbohydrates associated with placental mucin molecules are incomplete. Most of the results were obtained from histochemical studies and lectin-blotting, by combining antibodies specific for different mucin glycotopes with different plant lectins to decipher glycotope specificities.…”

Section: Trophoblast Mucinsmentioning

confidence: 99%

Interaction of extravillous trophoblast galectin-1 and mucin(s)—Is there a functional relevance?

Bojić‐Trbojević

Krivokuća

Kolundžić

et al. 2016

Cell Adhesion & Migration

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In the course of embryo implantation extensive interaction of the trophoblast with uterine tissue is crucial for adequate trophoblast invasion. This interaction is highly controlled, and it has been pointed out that a specific glycocode and changes in glycosylation may be important for successful implantation and maintenance of pregnancy. Both uterine and trophoblast cells have been shown to express cell surface glycoconjugates and sugar binding proteins, such as mucins (MUC) and galectins (gals). An increasing number of studies have investigated potential candidates interacting in this process. However, knowledge about the biochemical nature of the interactions and their importance for trophoblast cell function, and, consequently, for pregnancy outcome are still lacking. This review is aimed at deliberating the possibility that mucins, as heavily glycosylated proteins, might be among the functionally relevant galectin ligands in human trophoblast, based on both published data and our original research.

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“…Significant upregulation of the transmembrane mucin MUC1 (Hey et al 1994, 1995, Meseguer et al 2001 and its associated glycans keratan sulphate (Hoadley et al 1990, sialyl lewis x and Dolichos biflorus agglutinin (DBA)-binding ligands (Aplin 1991, Jones et al 1998, Campbell et al 2000 occurs during the secretory phase of the menstrual cycle, and might play a role in mediating the initial embryo-uterine epithelial interaction. During embryo attachment, the glycocalyx is reduced in thickness by a locally acting mechanism (Thathiah & Carson 2004, Horne et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Early stages of implantation as revealed by an in vitro model

Singh¹,

Nardo²,

Kimber³

et al. 2010

Reproduction

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Our limited understanding of the processes underlying steroid hormonal control of human endometrial receptivity is largely due to the lack of a relevant model system. To overcome scarcity of material, we have developed a model in which mouse embryos attach to human Ishikawa cells, which express functional steroid hormone receptors. Blastocysts flushed from day 4 pregnant superovulated mice were transferred to confluent Ishikawa cell monolayers. After 48 h of co-culture, 85% of the blastocysts had attached loosely, but only 40% attached stably to the epithelial cell surface. In contrast, 95% of the embryos attached stably to tissue culture plastic. Thus, weak attachment of a majority of the embryos was followed by stronger adhesion of a smaller proportion. Seventeen percent of the transferred blastocysts modified the epithelial cell surface with loss of MUC1 at the attachment site, extending variably to adjacent epithelial cells. Initially, stable attachment occurred without disruption to the integrity of the epithelial monolayer, but at later stages after the embryo had spread laterally, displacement of subjacent cells was observed. A modest increase in stable attachment, but no changes to MUC1 clearance, was observed after assisted hatching. After 24 h priming of Ishikawa cells by 17b-oestradiol (OE 2 ) followed by 72-h incubation with medroxyprogesterone acetate and OE 2 , stable attachment increased from 40 to 70%. Initial attachment is efficient either in the presence or in the absence of hormone; steroid treatment increased the incidence of stable attachment. Implantation failure is predicted to occur in this model when embryos fail to progress from initial to stable attachment.

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Glycans as biochemical markers of human endometrial secretory differentiation

Cited by 55 publications

References 37 publications

Lectin binding sites as markers of neonatal porcine uterine development.

Lectin binding sites as markers of neonatal porcine uterine development.

Interaction of extravillous trophoblast galectin-1 and mucin(s)—Is there a functional relevance?

Early stages of implantation as revealed by an in vitro model

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