Lectin binding sites as markers of neonatal porcine uterine development.

Spencer, Thomas E.; Wiley, Anne A.; Bartol, Frank F.

doi:10.1177/40.12.1453010

Cited by 11 publications

(11 citation statements)

References 16 publications

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“…Collectively, these and other observations from this laboratory [61] provide the basis for identification of three periods of neonatal porcine endometrial development (infantile, < Day 7; proliferative, Day 7-14; growth, > Day 14), for which specific histoarchitectural, cellular, and histochemical indices have been identified. The extent to which success of any of these events may be essential to insure functional competence of adult uterine tissues remains unknown.…”

Section: Discussionmentioning

confidence: 75%

Neonatal Porcine Endometrial Development Involves Coordinated Changes in DNA Synthesis, Glycosaminoglycan Distribution, and 3H-Glucosamine Labeling1

Spencer

Bartol

Wiley

et al. 1993

Biology of Reproduction

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To determine whether neonatal porcine endometrial development involved alterations in endometrial DNA synthesis, glycosaminoglycan (GAG) distribution, and/or 3H-glucosamine labeling, gilts were assigned randomly at birth (Day 0) to be hysterectomized on either Day 0, 7, 14, 28, 42, or 56. Uteri were processed for histology and Alcian blue-8GX (AB) histochemistry or cultured as explants in the presence of [methyl-3H]thymidine (3H-Thd) or D-[6-3H]glucosamine (3H-GlcN) and processed for subsequent autoradiography. For 3H-Thd-labeled tissues, labeling index (LI; % of nuclei labeled) was determined for two endometrial tissues (epithelium and stroma); two epithelial areas (luminal and glandular); and, for glandular epithelium, in three endometrial zones (zone 1 = shallow, zone 2 = intermediate, zone 3 = deep). For 3H-GlcN-labeled tissues, LI (grains/100 microns2) was determined for two stromal zones (shallow and deep). Endometrial glands were absent on Day 0, present in shallow stroma on Days 7 and 14, and extended to the myometrium in tissues from Day 28 through Day 56. Appearance of endometrial glands was associated with a dramatic increase in glandular epithelial 3H-Thd LI, which was maximal on Days 7 and 14 and declined thereafter. When glands were present in all three endometrial zones (Days 28-56), glandular epithelial 3H-Thd LI was consistently greatest in zone 2. Stromal 3H-Thd LI decreased after Day 0. In tissues obtained after Day 0, a distinct zone of alcianophilia was observed in shallow stroma adjacent to luminal epithelium and surrounding the necks of newly developed endometrial glands. This staining pattern was marked in tissues from Days 7, 14, and 28. Generally, stromal 3H-GlcN LI was greater in shallow than in deep stromal zones; it decreased after Day 0 to minimum values on Days 28 and 42 in both zones, and increased slightly in shallow stroma on Day 56. Data indicate that development of the neonatal porcine endometrium between birth and Day 56 involves coordinated alterations in patterns of DNA synthesis, GAG distribution, and glycoconjugate biosynthesis. The morphogenetic processes characterized here are likely to be regulated locally via changes in tissue microenvironment.

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Section: Discussionmentioning

confidence: 75%

Neonatal Porcine Endometrial Development Involves Coordinated Changes in DNA Synthesis, Glycosaminoglycan Distribution, and 3H-Glucosamine Labeling1

Spencer

Bartol

Wiley

et al. 1993

Biology of Reproduction

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“…Characteristics of neonatal uterine development between birth and puberty have been described at gross [10,14,23], histological [10-12, 15, 24], and biochemical levels [10, 15,16,18,25] in occidental breeds of pigs. Neonatal porcine uterine growth, as measured by uterine horn length and weight, occurs in an ovary-independent manner before, and in an ovary-dependent manner after, Day 60 of neonatal life [10,23].…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of Uterine Morphogenesis and Protein Secretion in Neonatal White Crossbred and Meishan Gilts1

Christenson¹,

Bartol²,

Vallet³

et al. 1997

Biology of Reproduction

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Thirty-five crossbred and 22 Meishan contemporary gilts were necropsied on Day 1, 14, 28, 42, or 56 of age (birth = Day 0). At necropsy, a cross section of one uterine horn was fixed for histomorphometric study, and minced uterine tissue was cultured with 50 muCi [3H]leucine. Secreted proteins were identified by two-dimensional PAGE, fluorography, and incorporation of radioactivity. Body weights at necropsy were similar for the two breeds and increased (p < 0.01) between Days 1 and 56 of age. Ovarian and uterine weights, as well as histomorphometric areas, were similar for the two breeds on Day 1 but increased markedly (p < 0.01) in Meishan gilts on Day 56. In gilts of both breeds, secretion of uterine proteins 1 (M(r) x 10(-3)/pI; 45/6.0), 2a and 2b (doublet, 25/6.2), and 3 (20/5.5) increased in association with endometrial gland development. A fourth protein (97/4.0) was observed in gilts of both breeds but was more abundant in Meishan; a fifth protein (13/6.0) was detected only in crossbred gilts on Day 56. Although specific regulatory roles for locally produced uterine proteins remain to be defined, the increase in specific uterine proteins and breed differences in uterine protein secretion suggest that uterine proteins may influence early uterine development.

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“…These staining patterns are unique compared with those in other reported functional stages of the uterus. DBA lectin binding is prominent on porcine uterine epithelia throughout GE adenogenesis, and actually increases during the luteal phase of the estrous cycle (Spencer et al 1992, Sant'Ana et al 2009). Similarly, a v has high expression at the apical surfaces of LE and GE during the porcine periimplantation period.…”

Section: Progesterone Effects On Development Of Pig Gementioning

confidence: 99%

Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy

Bailey¹,

Dunlap²,

Frank³

et al. 2010

Reproduction

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In pigs, endometrial functions are regulated primarily by progesterone and placental factors including estrogen. Progesterone levels are high throughout pregnancy to stimulate and maintain secretion of histotroph from uterine epithelia necessary for growth, implantation, placentation, and development of the conceptus (embryo and its extra-embryonic membranes). This study determined effects of longterm progesterone on development and histoarchitecture of endometrial luminal epithelium (LE), glandular epithelium (GE), and vasculature in pigs. Pigs were ovariectomized during diestrus (day 12), and then received daily injections of either corn oil or progesterone for 28 days. Prolonged progesterone treatment resulted in increased weight and length of the uterine horns, and thickness of the endometrium and myometrium. Hyperplasia and hypertrophy of GE were not evident, but LE cell height increased, suggesting elevated secretory activity. Although GE development was deficient, progesterone supported increased endometrial angiogenesis comparable to that of pregnancy. Progesterone also supported alterations to the apical and basolateral domains of LE and GE. Dolichos biflorus agglutinin lectin binding and a v integrin were downregulated at the apical surfaces of LE and GE. Claudin-4, a 2 b 1 integrin, and vimentin were increased at basolateral surfaces, whereas occludins-1 and -2, claudin-3, and E-cadherin were unaffected by progesterone treatment indicating structurally competent trans-epithelial adhesion and tight junctional complexes. Collectively, the results suggest that progesterone affects LE, GE, and vascular development and histoarchitecture, but in the absence of ovarian or placental factors, it does not support development of GE comparable to pregnancy. Furthermore, LE and vascular development are highly responsive to the effects of progesterone.

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Lectin binding sites as markers of neonatal porcine uterine development.

Cited by 11 publications

References 16 publications

Neonatal Porcine Endometrial Development Involves Coordinated Changes in DNA Synthesis, Glycosaminoglycan Distribution, and 3H-Glucosamine Labeling1

Neonatal Porcine Endometrial Development Involves Coordinated Changes in DNA Synthesis, Glycosaminoglycan Distribution, and 3H-Glucosamine Labeling1

Comparative Study of Uterine Morphogenesis and Protein Secretion in Neonatal White Crossbred and Meishan Gilts1

Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy

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