Glycans and glycoproteins as specific biomarkers for cancer

Kailemia, Muchena J.; Park, Dayoung; Lebrilla, Carlito B.

doi:10.1007/s00216-016-9880-6

Cited by 292 publications

(243 citation statements)

References 143 publications

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“…For example, glycosylation affects the safety and efficacy of biological therapeutics and serves as a biomarker of disease. [3–5] The N-glycans that are associated with dysfunction arise from alterations of a predictable base structure that contains a mannose core attached to N-acetylglucosamine linked to galactose [6]. In glycoproteins found in animals, galactose residues are generally linked to an N -acetylglucosamine residue through a β(1-4) linkage, (Galβ(1-4)GlcNAc).…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis with stationary nanogel zones of galactosidase and Erythrina cristagalli lectin for the determination of β(1–3)-linked galactose in glycans

Holland

Gattu

Crihfield

et al. 2017

Journal of Chromatography A

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A thermally responsive nanogel is used to create stationary zones of enzyme and lectin in a separation capillary. Once patterned in the capillary, analyte is driven through the zone, where it is converted to a specific product if an enzyme is used or captured if a lectin is used. These stationary zones are easily expelled after the analysis and then re-patterned in the capillary. The nanogel is compatible with enzymes and lectins and improves the stability of galactosidase, enabling more cost-effective use of biological reagents that provide insight into glycan structure. A feature of using stationary zones is that the reaction time can be controlled by the length of the zone, the applied field controlling the analyte mobility, or the use of electrophoretic mixing by switching the polarity of the applied voltage while the analyte is located in the zone. The temperature, applied voltage, and length of the stationary zone, which are factors that enhance the performance of the enzyme, are characterized. The combined use of enzymes and lectins in capillary electrophoresis is a new strategy to advance rapid and automated analyses of glycans using nanoliter volumes of enzymes and lectins. The applicability of this use of stationary zones of enzyme and lectin in capillary electrophoresis is demonstrated with the identification of β(1-3)-linked galactose in N-glycan.

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Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis with stationary nanogel zones of galactosidase and Erythrina cristagalli lectin for the determination of β(1–3)-linked galactose in glycans

Holland

Gattu

Crihfield

et al. 2017

Journal of Chromatography A

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“…[10] Sialylated glycans and glycoproteins have attracted special attention based on observations that they are upregulated in numerous cancers and, in circulation, have the potential to serve as biomarkers of disease. [11] …”

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confidence: 99%

Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomics

et al. 2017

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Sialylated glycans are found at elevated levels in many types of cancer and have been implicated in disease progression. However, the specific glycoproteins that contribute to the cancer cell-surface sialylation are not well characterized, specifically in bona fide human disease tissue. Metabolic and bioorthogonal labeling methods have previously enabled the enrichment and identification of sialoglycoproteins from cultured cells and model organisms. Herein, we report the first application of this glycoproteomic platform to human tissues cultured ex vivo. Both normal and cancerous prostate tissues were sliced and cultured in the presence of the azide-functionalized sialic acid biosynthetic precursor Ac4ManNAz. The compound was metabolized to the azidosialic acid and incorporated into cell surface and secreted sialoglycoproteins. Chemical biotinylation followed by enrichment and mass spectrometry led to the identification of glycoproteins that were found at elevated levels or uniquely in cancerous prostate tissue. This work therefore extends the use of bioorthogonal labeling strategies to problems of clinical relevance.

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“…At present, glycans have been used as candidate diagnostic markers and therapeutic targets in clinics (19,20). The modulation of N-Glycan biosynthesis (P value = 6.24e-14, ranked twelfth) changed the glycosylation of proteins and/or lipids, which made the functions and structures of glycoproteins and/or glycolipids change.…”

Section: Discussionmentioning

confidence: 99%

Cross-Sectional Study of Gene Expression Analysis Identifies Critical Biological Pathways and Key Genes Implicated in Non-Small Cell Lung Cancer

Wang

et al. 2018

Iran Red Crescent Med J

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Background: Non-small cell lung cancer (NSCLC) is the most common type of lung cancer, which accounts for about 85% of all lung cancer types. However, critical biological pathways and key genes implicated in NSCLC remain ambiguous. Objectives: The present study aimed at identifying the critical biological pathways and key genes implicated in NSCLC, and providing insight in the molecular mechanism underlying NSCLC. Methods: In this case-control bioinformatics study, the researchers used four microarray data of NSCLC from public gene expression omnibus (GEO) database at the national center for biotechnology information (NCBI) website. The microarray data came from studies of American, Spanish, and Taiwanese NSCLC patients, and in total contained 190 NSCLC tissue and 180 normal lung tissue. A standardized microarray preprocessing and gene set enrichment analysis (GSEA) were used to analyze each microarray data and obtained significantly regulated pathways. Venn analysis was used to identify the common significantly regulated biological pathways. Protein and protein interaction (PPI) network analysis was used to identify the key genes within common significantly regulated pathways. The PPI information was retrieved from STRING database, and cytoscape software was used to construct and visualize the PPI network. Results: Through integrating GSEA results of four microarray data, finally, the researchers identified 22 common up-regulated and 85 common down-regulated pathways. Many genes within 107 common significantly regulated pathways were significantly enriched within cell cycle pathway (P value of 2.58e-79) and focal adhesion pathway (P value of 2.44e-81). The PPI network showed that up-regulated CDK1 (P value = 1.33e-18 and logFC = 1.41) and down-regulated PIK3R1 (P value = 5.09e-22 and logFC = -1.13) genes shared the most abundant edges, and were associated with NSCLC. Conclusions: This cross-sectional study showed increased concordance between gene expression profiling data. These identified pathways and genes provide some insight in the molecular mechanisms of NSCLC, and the genes may serve as candidate diagnostic and therapeutic targets of NSCLC.

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Glycans and glycoproteins as specific biomarkers for cancer

Cited by 292 publications

References 143 publications

Capillary electrophoresis with stationary nanogel zones of galactosidase and Erythrina cristagalli lectin for the determination of β(1–3)-linked galactose in glycans

Capillary electrophoresis with stationary nanogel zones of galactosidase and Erythrina cristagalli lectin for the determination of β(1–3)-linked galactose in glycans

Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomics

Cross-Sectional Study of Gene Expression Analysis Identifies Critical Biological Pathways and Key Genes Implicated in Non-Small Cell Lung Cancer

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