Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides

Shu, Jingjing; Xiong, Wenli; Zhang, Ran; Ma, Shanyun; Zhou, Kaiqiang; Wang, Xuwei; Yan, F.; Huang, Da; Li, Jianhua; Wu, Yuanzi; He, Jianan

doi:10.1016/j.talanta.2022.123956

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…Site-specific conjugation of the polymer to protein generally involves additional preprocessing steps and a limited amount of grafting. To overcome these shortcomings, we have reported a versatile approach to overcome these shortcomings through the multivalent conjugation of polymers to the glycan moieties of glycoproteins. , Briefly, this approach begins with gently oxidizing glycans on GOx with sodium periodate to produce ketones (GOx-Oxi) on the glycan moieties . The initiator ABM specifically reacted with the ketones to form a macroinitiator (GOx-ABM).…”

Section: Resultsmentioning

confidence: 99%

Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing

Wu,

Lyu,

et al. 2023

Biomacromolecules

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An abnormal microenvironment underlies poor healing in chronic diabetic chronic wounds. However, effectively modulating the microenvironment of the diabetic wound remains a great challenge due to sustained oxidative stress and chronic inflammation. Here, we present a unimolecular enzyme−polymer conjugate that demonstrates excellent multienzymatic cascade activities. The cascaded enzyme conjugates (CECs) were synthesized by grafting poly(N-acryloyl-lysine) (pLAAm) from the glycan moieties of glucose oxidase (GOx) via glycan-initiated polymerization. The resulting CECs exhibited multiple enzymatic properties of GOx, superoxide dismutase mimic, and catalase mimic activities simultaneously. The CECs facilitated the depletion of high blood glucose, ROS scavenging, bacteria-killing, antiinflammatory effects, and sustained oxygen generation, which restored the microenvironment in diabetic wounds. In vivo results from a diabetic mouse model confirmed the capacity and efficiency of the cascade reaction for diabetic wound healing. Our findings demonstrate that the three-in-one enzyme−polymer conjugates alone can modulate the diabetic microenvironment for wound healing.

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Section: Resultsmentioning

confidence: 99%

Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing

Wu,

Lyu,

et al. 2023

Biomacromolecules

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“…12,13 Currently, the technologies for the enrichment of N-glycopeptides include the boric acid method, 14 lectin affinity chromatography, 15 hydrophilic interaction (HILIC) method, 16,17 and hydrazide chemistry. 18 Among them, the HILIC method has the advantages of unbiased enrichment ability, high sensitivity, and simple operation. 16,17 Hydrophilic materials such as maltose, 19 L-cysteine, 20 glutathione, 21 and organic frameworks 22,23 have been extensively used for N-glycopeptide enrichment.…”

Section: Introductionmentioning

confidence: 99%

In situ grown magnetic COF@MOF with a phosphoserine anchor for in-depth N-glycopeptide analysis in serum

Guo,

Hua,

Wang

et al. 2023

Analyst

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Dual-functionalized two-dimensional metal–organic framework composite with highly hydrophilicity for effective enrichment of glycopeptides

Yuan,

Song,

Wang

et al. 2024

Journal of Chromatography B

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Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides

Cited by 3 publications

References 47 publications

Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing

Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing

In situ grown magnetic COF@MOF with a phosphoserine anchor for in-depth N-glycopeptide analysis in serum

Dual-functionalized two-dimensional metal–organic framework composite with highly hydrophilicity for effective enrichment of glycopeptides

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