Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing

Wu, Yuanzi; Lyu, Yanwei; Li, Ling; Zhou, Kaiqiang; Cai, Jingwen; Wang, Xuwei; Wang, Huiru; Yan, Fen; Weng, Zuquan

doi:10.1021/acs.biomac.3c00698

Cited by 3 publications

(2 citation statements)

References 59 publications

(90 reference statements)

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“…Other experiments also demonstrated favorable SOD and CAT activities of CHA@GOx, which synergistically regulated the ROS and oxygen balance of the wound with the oxidation of glucose and reduced the toxic side effects of GOx. Wu et al reported excellent multienzymatic activity with a single-molecule cascade multienzymatic conjugate. The cascade enzyme conjugates (CECs) were synthesized by polymerizing the glycan of GOx and ligating it with ferrous ions (Fe 2+ ).…”

Section: Applications Of Nanozymes In Dfu Healingmentioning

confidence: 99%

Nanozymes for the Therapeutic Treatment of Diabetic Foot Ulcers

Xiao,

Zhao,

DuBois

et al. 2024

ACS Biomater. Sci. Eng.

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Diabetic foot ulcers (DFU) are chronic, refractory wounds caused by diabetic neuropathy, vascular disease, and bacterial infection, and have become one of the most serious and persistent complications of diabetes mellitus because of their high incidence and difficulty in healing. Its malignancy results from a complex microenvironment that includes a series of unfriendly physiological states secondary to hyperglycemia, such as recurrent infections, excessive oxidative stress, persistent inflammation, and ischemia and hypoxia. However, current common clinical treatments, such as antibiotic therapy, insulin therapy, surgical debridement, and conventional wound dressings all have drawbacks, and suboptimal outcomes exacerbate the financial and physical burdens of diabetic patients. Therefore, development of new, effective and affordable treatments for DFU represents a top priority to improve the quality of life of diabetic patients. In recent years, nanozymes-based diabetic wound therapy systems have been attracting extensive interest by integrating the unique advantages of nanomaterials and natural enzymes. Compared with natural enzymes, nanozymes possess more stable catalytic activity, lower production cost and greater maneuverability. Remarkably, many nanozymes possess multienzyme activities that can cascade multiple enzymecatalyzed reactions simultaneously throughout the recovery process of DFU. Additionally, their favorable photothermal-acoustic properties can be exploited for further enhancement of the therapeutic effects. In this review we first describe the characteristic pathological microenvironment of DFU, then discuss the therapeutic mechanisms and applications of nanozymes in DFU healing, and finally, highlight the challenges and perspectives of nanozyme development for DFU treatment.

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Section: Applications Of Nanozymes In Dfu Healingmentioning

confidence: 99%

Nanozymes for the Therapeutic Treatment of Diabetic Foot Ulcers

Xiao,

Zhao,

DuBois

et al. 2024

ACS Biomater. Sci. Eng.

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“…18 It is believed that oxidative stress plays a vital role in diabetic and chronic wound healing. 19 The body produces too many reactive oxygen species due to an imbalance between free radicals and antioxidants, 20,21 which damages cells and tissues and slows the healing of wounds. 22–24 Antioxidant systems may help improve healing by lowering ROS levels and reducing oxidative stress-related damage.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a core–shell nanofibrous wound dressing with an antioxidant effect on skin injury

Feng,

Tang,

Qiu

et al. 2024

J. Mater. Chem. B

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Carbonized Polymer Dot‐Tannic Acid Nanoglue: Tissue Reinforcement with Concurrent Fluorescent Tracking, Insulin Delivery, and Reactive Oxygen Species Regulation for Normal and Diabetic Wound Healing

Aggarwal,

Sharda,

Srivastava

et al. 2024

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Nanotizing biosealant components offer a multitude of chemical functionalities for superior adhesion–cohesion, delivering unique properties for comprehensive wound healing that are otherwise impossible to achieve using commercial variants. For the first time, a two‐step controlled hydrothermal pyrolysis is reported to nanotize dopamine, phloroglucinol, and glutaraldehyde into carbon dot (CD) to be subsequently converted into carbonized polymer dot (CPD) with gelatin as a co‐substrate. Chemical crosslinking of CD with gelatin through Schiff base formation before the second pyrolysis step ensures a complex yet porous polymeric network. The retention of chemical functionalities indigenous to CD substrates and gelatin along with the preservation of CD photoluminescence in CPD for optical tracking is achieved. A unique nanoformulation is created with the CPD through tannic acid (TA) grafting evolving CPD‐TA nanoglue demonstrating ≈1.32 MPa strength in lap shear tests conducted on porcine skin, surpassing traditional bioadhesives. CPD‐TA nanoglue uploaded insulin as chosen cargo disbursal at the wound site for healing normal and in vitro diabetic wound models using HEKa cells with extraordinary biocompatibility. Most importantly, CPD‐TA can generate reactive oxygen species (ROS) and scavenge simultaneously under ambient conditions (23 W white LED or dark) for on‐demand sterilization or aiding wound recovery through ROS scavenging.

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Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing

Cited by 3 publications

References 59 publications

Nanozymes for the Therapeutic Treatment of Diabetic Foot Ulcers

Nanozymes for the Therapeutic Treatment of Diabetic Foot Ulcers

Fabrication of a core–shell nanofibrous wound dressing with an antioxidant effect on skin injury

Carbonized Polymer Dot‐Tannic Acid Nanoglue: Tissue Reinforcement with Concurrent Fluorescent Tracking, Insulin Delivery, and Reactive Oxygen Species Regulation for Normal and Diabetic Wound Healing

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