Glycan microarray profiling of parasite infection sera identifies the LDNF glycan as a potential antigen for serodiagnosis of trichinellosis

Abstract

Diagnostic methods for parasite infections still highly depend on the identification of the parasites by direct methods such as microscopic examination of blood, stool and tissue biopsies. Serodiagnosis is often carried out to complement the direct methods, however few synthetic antigens with sufficient sensitivity and specificity are available. Here we evaluated a glycan microarray approach to select for synthetic glycan antigens that could be used for serodiagnosis of parasitic infections. Using a glycan array containing over 250 different glycan antigens, we identified GalNAcβ1-4(Fucα1-3)GlcNAc-R (LDNF) as a glycan antigen that is recognized by antibodies from Trichinella-infected individuals. We synthesized a neoglycoconjugate, consisting of 5 LDNF molecules covalently coupled to bovine serum albumin (BSA), and used this neoglycoconjugate as an antigen to develop a highly sensitive total-Ig ELISA for serological screening of trichinellosis. The results indicate that glycan microarrays constitute a promising technology for fast and specific identification of parasite glycan antigens to improve serodiagnosis of different parasitic infections, either using an ELISA format, or parasite-specific glycan-arrays.

Chagas Disease Diagnostic Applications

“…These methodologies have been successfully explored in different parasitic infections (Anish et al, 2013; Aranzamendi et al, 2011; Martin et al, 2013). …”

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

“…To investigate mechanisms of molecular chaperones (Amin et al, 2011) GalNAc 1 4(Fuc 1 3)GlcNAc-R BSA TOF/TOF Glycan array used to identify glycan as potential antigen for serodiagnosis of trichinellosis (Aranzamendi, et al, 2011) Glucose/cholesterol BSA TOF (sinapinic) Cholesterol promotes formation of AGEs (Hirasawa et al, 2011) Glyco-PEG Factor VIIa L-TOF (CHCA)…”