GLUTs and Mammalian Sperm Metabolism

Bucci, Diego; Rodríguez‐Gil, Joan E.; Vallorani, Claudia; Spinaci, Marcella; Galeati, Giovanna; Tamanini, Carlo

doi:10.2164/jandrol.110.011197

Cited by 85 publications

(87 citation statements)

References 66 publications

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“…Although proteins of the SGLT family have been reported to be present in mammalian sperm [19], at this moment, there is a general consensus in considering that GLUT proteins are the most important hexose uptake mechanism of mammalian sperm [20].…”

Section: Overview Of Mammalian Sperm Energy Metabolismmentioning

confidence: 99%

“…These proteins differentiate themselves by subtle structural changes that, in turn, lead to great differences in the affinity for separate sugars and tissue specificity [17,18]. Regarding mammalian spermatozoa, the GLUTs that have been detected until now comprise GLUT-1, GLUT-2, GLUT-3, GLUT-5, GLUT-8, and GLUT-9, whereas the presence of the insulin-dependent GLUT-4 seems doubtful [20]. An intriguing question regarding GLUTs is why a cell, such as the mature mammalian spermatozoon, requires the presence of at least six separate GLUT hexose transporters to uptake a very common external energy sources such as monosaccharides.…”

Section: Overview Of Mammalian Sperm Energy Metabolismmentioning

confidence: 99%

See 1 more Smart Citation

Current knowledge on boar sperm metabolism: Comparison with other mammalian species

Rodríguez-Gil

Bonet

2016

Theriogenology

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Section: Overview Of Mammalian Sperm Energy Metabolismmentioning

confidence: 99%

Section: Overview Of Mammalian Sperm Energy Metabolismmentioning

confidence: 99%

Current knowledge on boar sperm metabolism: Comparison with other mammalian species

Rodríguez-Gil

Bonet

2016

Theriogenology

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“…DIET AND EXERCISE IMPROVE SPERM FUNCTION IN OBESE MICE energy source (15). It has been found that glucose, not fructose, is needed for fertilization and is specifically required during sperm oocyte binding and embryo viability in the mouse (72).…”

Section: E776mentioning

confidence: 99%

Diet and exercise in an obese mouse fed a high-fat diet improve metabolic health and reverse perturbed sperm function

Palmer¹,

Bakos

Owens³

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

194

181

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Palmer NO, Bakos HW, Owens JA, Setchell BP, Lane M. Diet and exercise in an obese mouse fed a high-fat diet improve metabolic health and reverse perturbed sperm function. Am J Physiol Endocrinol Metab 302: E768 -E780, 2012. First published January 17, 2012; doi:10.1152/ajpendo.00401.2011.-Male obesity is associated with reduced sperm motility and morphology and increased sperm DNA damage and oxidative stress; however, the reversibility of these phenotypes has never been studied. Therefore, the aim of this study was to assess the reversibility of obesity and its associated sperm physiology and function in mice in response to weight loss through diet and exercise. C57BL6 male mice (n ϭ 40) were fed either a control diet (CD; 6% fat) or a high-fat diet (HFD; 21% fat) for 10 wk before allocation to either diet and/or swimming exercise interventions for 8 wk. Diet alone reduced adiposity (1.6-fold) and serum cholesterol levels (1.7-fold, P Ͻ 0.05), while exercise alone did not alter these, but exercise plus diet also improved glucose tolerance (1.3-fold, P Ͻ 0.05). Diet and/or exercise improved sperm motility (1.2-fold) and morphology (1.1-fold, P Ͻ 0.05), and reduced sperm DNA damage (1.5-fold), reactive oxygen species (1.1-fold), and mitochondrial membrane potential (1.2-fold, P Ͻ 0.05) and increased sperm binding (1.4-fold) (P Ͻ 0.05). Sperm parameters were highly correlated with measures of glycemia, insulin action, and serum cholesterol (all P Ͻ 0.05) regardless of adiposity or intervention, suggesting a link between systemic metabolic status and sperm function. This is the first study to show that the abnormal sperm physiology resulting from obesity can be reversed through diet and exercise, even in the presence of ongoing obesity, suggesting that diet and lifestyle interventions could be a combined approach to target subfertility in overweight and obese men. lifestyle interventions; fertility and metabolism OBESITY IS A GLOBAL HEALTH PROBLEM that is reaching epidemic proportions, with 1.6 billion adults classified as overweight and 400 million adults classified as obese worldwide (60). Consequently, the rates of male obesity in reproductive age men has nearly tripled since the 1970s (33). Recent studies in both humans and other species shows that male obesity impairs male reproductive potential, affecting both the molecular and physical structure of sperm (1,10,16,20,21,34,35,38,45,47,50,63,75,79) and the health of the developing fetus and subsequent offspring (9,24,42,55,59).Although fertility is not always impaired in obese men, almost 80% of men that present to fertility clinics are classified as either overweight or obese, suggestive of an interaction between obesity and sperm function (9). There is a negative impact of male obesity on standard semen parameters, including motility, count, and hormone abundance, such as testosterone and inhibin B (1,5,16,18,21,22,32,35,37,45,47,52,62,69,85). Further studies have reported reduced pregnancy success and live birth outcomes after IVF treatment in overweight and...

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“…In fact, mammalian sperm require metabolic energy for a variety of functions (Williams & Ford 2001, Ferramosca & Zara 2014, and it is known that human spermatozoa obtain a significant proportion of their energy from glycolysis (Williams & Ford 2001). Nevertheless, the origin of ATP for sperm motility is still being intensely discussed (Ford & Harrison 1981, Williams & Ford 2001, Ruiz-Pesini et al 2007, Bucci et al 2011, Piomboni et al 2012.…”

Section: Effects Of Substrate Availability On Spermatozoa Functionalitymentioning

confidence: 99%

High glucose concentrations per se do not adversely affect human sperm function in vitro

et al. 2015

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Diabetes mellitus (DM) represents one of the greatest concerns to global health and it is associated with diverse clinical complications, including reproductive dysfunction. Given the multifactorial nature of DM, the mechanisms that underlie reproductive dysfunction remain unclear. Considering that hyperglycemia has been described as a major effector of the disease pathophysiology, we used an in vitro approach to address the isolated effect of high glucose conditions on human sperm function, thus avoiding other in vivo confounding players. We performed a complete and integrated analysis by measuring a variety of important indicators of spermatozoa functionality (such as motility, viability, capacitation status, acrosomal integrity, mitochondrial superoxide production and membrane potential) in human sperm samples after incubation with D-and L-glucose (5, 25, or 50 mM) for 24 and 48 h. No direct effects promoted by 25 or 50 mM D-glucose were found for any of the parameters assessed (PO0.05), except for the acrosome reaction, which was potentiated after 48 h of exposure to 50 mM D-glucose (P!0.05). Interestingly, non-metabolizable L-glucose drastically increased superoxide production (P!0.05) and suppressed sperm motility (P!0.05) and capacitation (P!0.05) after 24 h of treatment, whereas mitochondrial membrane potential (P!0.05), acrosomal integrity (P!0.01) and viability (P!0.05) were later decreased. The overall results suggest that high glucose levels per se do not influence human sperm function in vitro, which stresses the importance of other factors involved in DM pathology. Nevertheless, the absence of metabolizable glucose contributes to a severe impairment of sperm function and thus compromises male fertility. Free Portuguese abstract: A Portuguese translation of this abstract is freely available at

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GLUTs and Mammalian Sperm Metabolism

Cited by 85 publications

References 66 publications

Current knowledge on boar sperm metabolism: Comparison with other mammalian species

Current knowledge on boar sperm metabolism: Comparison with other mammalian species

Diet and exercise in an obese mouse fed a high-fat diet improve metabolic health and reverse perturbed sperm function

High glucose concentrations per se do not adversely affect human sperm function in vitro

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