Current knowledge on boar sperm metabolism: Comparison with other mammalian species

Rodríguez-Gil, Joan E.; Bonet, Sergi

doi:10.1016/j.theriogenology.2015.05.005

Cited by 50 publications

(42 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These differences may be due to the unique characteristics of the boar sperm. For example, the motility of the pig sperm after ejaculation is slower than in other species (e.g., horse and cattle), while it is also very prone to be altered by a myriad of environmental incidences (Rodríguez-Gil and Bonet 2015). In light of these singularities, we addressed the question: which sperm quality factors are influencing SRR?…”

Section: Discussionmentioning

confidence: 99%

A technical assessment of the porcine ejaculated spermatozoa for a sperm-specific RNA-seq analysis

Gòdia

Mayer

Nafissi

et al. 2018

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

FPKM: fragments per kilobase of transcript per million mapped reads; KRT1: keratin 1; miRNA: micro-RNA; miscRNA: miscellaneous RNA; Mt rRNA: mitochondrial ribosomal RNA; Mt tRNA: mitochondrial transference RNA; OAZ3: ornithine decarboxylase antizyme 3; ORT: osmotic resistance test; piRNA: Piwi-interacting RNA; PRM1: protamine 1; PTPRC: protein tyrosine phosphatase receptor type C; rRNA: ribosomal RNA; snoRNA: small nucleolar RNA; snRNA: small nuclear RNA; SRR: sperm recovery rate; tRNA: transfer RNA.

show abstract

Section: Discussionmentioning

confidence: 99%

A technical assessment of the porcine ejaculated spermatozoa for a sperm-specific RNA-seq analysis

Gòdia

Mayer

Nafissi

et al. 2018

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…Exposure of boar spermatozoa to the μ‐agonist morphine reduces mitochondrial function, despite the maintained or elevated sperm motility. At face value, this would be a paradox; yet boar spermatozoa consume an abundance of glucose through glycolysis and respiratory oxidation (Rodriguez‐Gil and Bonet, ), so decreased mitochondrial integrity might not lead to an immediate energy deficit.…”

Section: Discussionmentioning

confidence: 99%

The mu (μ) and delta (δ) opioid receptors modulate boar sperm motility

Vicente-Carrillo

Álvarez‐Rodríguez

Rodriguez‐Martinez

2016

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…In vitro assays to assess different sperm functions are, at present, a very important tool to understand and discover the pathways implicated in sperm function and their changes. Different approaches are used nowadays to assess sperm features in vitro: sperm motility analysis systems (Amann and Katz, 2004;Hoogewijs et al, 2012;Boryshpolets et al, 2013;Amann and Waberski, 2014), morphology (Morrell, 2006;Love, 2011), fluorescent microscopy (Mattioli et al, 1996;Gravance et al, 2000;Flesch et al, 2001;Guthrie et al, 2008;Ortega Ferrusola et al, 2009;Satorre et al, 2009;Kim et al, 2011), protein analysis (Spinaci et al, 2005b;Krisfalusi et al, 2006;Gadella, 2008;Flores et al, 2011;Kumaresan et al, 2011), flow cytometry (Didion et al, 2009;Martìnez-Pastor et al, 2010;Petrunkina and Harrison, 2010;Hossain et al, 2011;Gürler et al, 2015;Barrier Battut et al, 2016;Battut et al, 2017), metabolic assay (Albarracín et al, 2004;Storey, 2008;Bucci et al, 2011;Gibb and Aitken, 2016;Rodríguez-Gil and Bonet, 2016); sperm oocyte interaction (Funahashi et al, 1997;Sinowatz et al, 2003;McPartlin et al, 2008;Mugnier et al, 2009; Balao da Silva et al, 2013;Plaza Dávila et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Different approaches for assessing sperm function

Bucci¹,

Spinaci²,

Galeati³

et al. 2019

Anim Reprod

View full text Add to dashboard Cite

Different approaches can be used to assess sperm function in different conditions, i.e. sperm storage, freezing-thawing or activation by induction of capacitation and acrosome reaction. In this review we will focus on the assays routinely performed in our laboratories, giving a literature support to critically analyse different approaches. In fact, researchers usually tend to look for the "one shot" parameter that could explain itself a specific process; it is our conviction that a multiparametric approach is still more valid, as some changes in sperm function are very complex and could be explained only by operating in different ways. Sperm motility, the most evident sperm characteristic, should be assessed by computer-aided sperm analysers that permit an objective evaluation of the motility and its kinematic parameters. Commercial and open source instruments are available and could be profitably used together with specific statistical approaches. The use of microscopy, and particularly fluorescent microscopy, could be a very useful tool to assess different parameters in sperm cells both by fluorophores that give indication of a determined function, and by immunolocalization of proteins, that permits the discover of new features or to explain particular sperm functions. The same substrates could be used also in flow cytometry: the difference is that it permits to study wider sperm populations (and their subpopulation distribution). Flow cytometry is undergoing a very wide use in spermatology and technical and experimental rigor is needed to obtain reliable results. Metabolic assessment of sperm features, particularly energetic supply, ATP formation and other enzyme activities, could represent a very important challenge to acquire new information and complete/integrate those derived from other techniques. Finally, functional assays such as oocyte binding and in vitro fertilization, represent a very strong tool to assess sperm function in vitro, as they could evidence the functional intactness of some pathways.

show abstract

Current knowledge on boar sperm metabolism: Comparison with other mammalian species

Cited by 50 publications

References 61 publications

A technical assessment of the porcine ejaculated spermatozoa for a sperm-specific RNA-seq analysis

A technical assessment of the porcine ejaculated spermatozoa for a sperm-specific RNA-seq analysis

The mu (μ) and delta (δ) opioid receptors modulate boar sperm motility

Different approaches for assessing sperm function

Contact Info

Product

Resources

About