Glutathionylation: a regulatory role of glutathione in physiological processes

Dominko, Kristina; Đikić, Domagoj

doi:10.2478/aiht-2018-69-2966

Cited by 47 publications

(39 citation statements)

References 221 publications

(375 reference statements)

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“…Except for glutathione, GDAS includes enzymes such as GP, GR, GT etc. [47,48]. In this study, GP activity decreased in MIA-OA group in 1.5 times, compared to control group (Table 3).…”

Section: Resultssupporting

confidence: 49%

Effect of probiotic composition on oxidative/antioxidant balance in blood of rats under experimental osteoarthriti

Korotkyi¹,

Dvorshchenko²,

Vovk³

et al. 2019

Ukr.Biochem.J

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Osteoarthritis (OA) is a widespread pathology of the musculoskeletal system. OA may associate with a wide range of disorders, that lead to the development of various strategies on how to prevent and treat the disease. recent studies discussed interactions between the microbiome and a wide range of pathologies, including OA. In this study, we investigated the effect of probiotic cultures on oxidative/antioxidant balance in blood of rats during OA. Experimental OA was induced by a single injection of sodium monoiodoacetate into the knee joint. A probiotic composition (Multiprobiotic Simbiter®) was administered by peroral catheter once per day for 14 days. We investigated the next parameters: expression of Nos2 gene in the blood, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione transferase activity, glutathione reductase activity, contents of superoxide, hydrogen peroxide, TBA-reactive compounds, oxidized and reduced glutathione in the serum of the blood. Monoiodoacetate-induced OA caused significant changes on oxidative/antioxidant balance in the blood of rats: increasing of the contents of superoxide anion radical, hydrogen peroxide, thiobarbituric acid-reactive compounds, oxidized glutathione, upregulating of Nos2 expression, increasing of catalase activity; conversely, superoxide dismutase activity, glutathione peroxidize activity, glutathione transferase activity, glutathione reductase activity, the content of reduced glutathione were significantly decreased, compared to control group. Administration of probiotics to animals with OA led to positive changes in the studied parameters approaching the values of control group (some of them were statistically significant). K e y w o r d s: experimental osteoarthritis, microbiome, free radical processes, glutathione.

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“…Except for glutathione, GDAS includes enzymes such as GP, GR, GT etc. [47,48]. In this study, GP activity decreased in MIA-OA group in 1.5 times, compared to control group (Table 3).…”

Section: Resultssupporting

confidence: 49%

Effect of probiotic composition on oxidative/antioxidant balance in blood of rats under experimental osteoarthriti

Korotkyi¹,

Dvorshchenko²,

Vovk³

et al. 2019

Ukr.Biochem.J

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“…Glutathionylation of specific cysteine residues in mitogen-activated protein kinases (MAPK) [69][70][71] and PTPs [12,27,72] affect their transduction and regulatory properties and ultimately lead to an altered tight junction functionality. Both GSH depletion and GSSG accumulation seem to be crucial events in this mechanism [13,73]. Here, redox changes in target proteins, initiated by reactive oxygen species or GSH redox imbalance, often precede protein glutathionylation and affects the binding strength of GSH to a protein [74,75].…”

Section: Abrupt Weaning Was Associated With Barrier Disruption and Tmentioning

confidence: 99%

Changes of the glutathione redox system during the weaning transition in piglets, in relation to small intestinal morphology and barrier function

Degroote

Vergauwen

Wang

et al. 2020

J Animal Sci Biotechnol

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Background: Weaning is known to result in barrier dysfunction and villus atrophy in the immediate post-weaning phase, and the magnitude of these responses is hypothesized to correlate with changes in the glutathione (GSH) redox system. Therefore, these parameters were simultaneously measured throughout the weaning phase, in piglets differing in birth weight category and weaning age, as these pre-weaning factors are important determinants for the weaning transition. Low birth weight (LBW) and normal birth weight (NBW) littermates were assigned to one of three weaning treatments; i.e. weaning at 3 weeks of age (3w), weaning at 4 weeks of age (4w) and removal from the sow at 3 d of age and fed a milk replacer until weaning at 3 weeks of age (3d3w). For each of these treatments, six LBW and six NBW piglets were euthanized at 0, 2, 5, 12 or 28 d post-weaning piglets, adding up 180 piglets. Results: Weaning increased the glutathione peroxidase activity on d 5 post-weaning in plasma, and duodenal and jejunal mucosa. Small intestinal glutathione-S-transferase activity gradually increased until d 12 post-weaning, and this was combined with a progressive rise of mucosal GSH up till d 12 post-weaning. Oxidation of the GSH redox status (GSH/GSSG E h) was only observed in the small intestinal mucosa of 3d3w weaned piglets at d 5 postweaning. These piglets also demonstrated increased fluorescein isothiocyanate dextran (FD4) and horseradish peroxidase fluxes in the duodenum and distal jejunum during the experiment, and specifically demonstrated increased FD4 fluxes at d 2 to d 5 post-weaning. On the other hand, profound villus atrophy was observed during the weaning transition for all weaning treatments. Finally, LBW and NBW piglets did not demonstrate notable differences in GSH redox status, small intestinal barrier function and histo-morphology throughout the experiment. Conclusion: Although moderate changes in the GSH redox system were observed upon weaning, the GSH redox status remained at a steady state level in 3w and 4w weaned piglets and was therefore not associated with weaning induced villus atrophy. Conversely, 3d3w weaned piglets demonstrated GSH redox imbalance in the small intestinal mucosa, and this co-occurred with a temporal malfunction of their intestinal barrier function.

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“…Enzymes like leukotriene C4 synthase, prostaglandin‐endoperoxide synthase 1 or 2 and prostaglandin E synthase, require glutathione as a cofactor for their activity. These enzymes are part of the superfamily of large, membrane‐associated proteins in eicosanoid and glutathione metabolism (MAPEG), sharing structural evolutionary relationship with GSTs .…”

Section: Introductionmentioning

confidence: 99%

Glutathione compartmentalization and its role in glutathionylation and other regulatory processes of cellular pathways

et al. 2018

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Glutathione is considered the major non‐protein low molecular weight modulator of redox processes and the most important thiol reducing agent of the cell. The biosynthesis of glutathione occurs in the cytosol from its constituent amino acids, but this tripeptide is also present in the most important cellular districts, such as mitochondria, nucleus, and endoplasmic reticulum, thus playing a central role in several metabolic pathways and cytoprotection mechanisms. Indeed, glutathione is involved in the modulation of various cellular processes and, not by chance, it is a ubiquitous determinant for redox signaling, xenobiotic detoxification, and regulation of cell cycle and death programs. The balance between its concentration and redox state is due to a complex series of interactions between biosynthesis, utilization, degradation, and transport. All these factors are of great importance to understand the significance of cellular redox balance and its relationship with physiological responses and pathological conditions. The purpose of this review is to give an overview on glutathione cellular compartmentalization. Information on its subcellular distribution provides a deeper understanding of glutathione‐dependent processes and reflects the importance of compartmentalization in the regulation of specific cellular pathways. © 2018 BioFactors, 45(2):152–168, 2019

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Glutathionylation: a regulatory role of glutathione in physiological processes

Cited by 47 publications

References 221 publications

Effect of probiotic composition on oxidative/antioxidant balance in blood of rats under experimental osteoarthriti

Effect of probiotic composition on oxidative/antioxidant balance in blood of rats under experimental osteoarthriti

Changes of the glutathione redox system during the weaning transition in piglets, in relation to small intestinal morphology and barrier function

Glutathione compartmentalization and its role in glutathionylation and other regulatory processes of cellular pathways

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