Effect of probiotic composition on oxidative/antioxidant balance in blood of rats under experimental osteoarthriti

Abstract

Osteoarthritis (OA) is a widespread pathology of the musculoskeletal system. OA may associate with a wide range of disorders, that lead to the development of various strategies on how to prevent and treat the disease. recent studies discussed interactions between the microbiome and a wide range of pathologies, including OA. In this study, we investigated the effect of probiotic cultures on oxidative/antioxidant balance in blood of rats during OA. Experimental OA was induced by a single injection of sodium monoiodoacetate into the knee joint. A probiotic composition (Multiprobiotic Simbiter®) was administered by peroral catheter once per day for 14 days. We investigated the next parameters: expression of Nos2 gene in the blood, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione transferase activity, glutathione reductase activity, contents of superoxide, hydrogen peroxide, TBA-reactive compounds, oxidized and reduced glutathione in the serum of the blood. Monoiodoacetate-induced OA caused significant changes on oxidative/antioxidant balance in the blood of rats: increasing of the contents of superoxide anion radical, hydrogen peroxide, thiobarbituric acid-reactive compounds, oxidized glutathione, upregulating of Nos2 expression, increasing of catalase activity; conversely, superoxide dismutase activity, glutathione peroxidize activity, glutathione transferase activity, glutathione reductase activity, the content of reduced glutathione were significantly decreased, compared to control group. Administration of probiotics to animals with OA led to positive changes in the studied parameters approaching the values of control group (some of them were statistically significant). K e y w o r d s: experimental osteoarthritis, microbiome, free radical processes, glutathione.

Assessment of the Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells against a Monoiodoacetate-Induced Osteoarthritis Model in Wistar Rats

“…Korotkyi et al [ 59 ] demonstrated that MIA-OA induced free radical reactions that caused the accumulation of superoxide anion radicals, hydrogen peroxide, and NO and thiobarbituric acid-reactive compounds that are intermediate products of lipid peroxidation. Elevated oxidative stress and ROS levels triggered by MIA led to the activation of the intrinsic pathway through the depolarization of membrane potential, the promotion of the discharge of cytochrome c, and the activation of caspase-3 [ 10 , 60 , 61 ].…”

“…Caspase-3 contributes to the overall apoptotic process by cleaving various cellular substrates [ 62 ]. Korotkyi et al [ 59 ] also mentioned that MIA-induced OA led to a decrease in superoxide dismutase (SOD) activity of glutathione (GSH) which represents the first line of antioxidants that catalytically scavenge the free radicals [ 13 ].…”

“…MIA has been shown to have necrotic and proapoptotic effects on rat chondrocytes in vitro, whilst TNF-α activates the tumor necrosis factor receptor (TNFR) or death receptors which eventually triggers the extrinsic pathway of apoptosis (Figure 10). Korotkyi et al [59] demonstrated that MIA-OA induced free radical reactions that caused the accumulation of superoxide anion radicals, hydrogen peroxide, and NO and thiobarbituric acid-reactive compounds that are intermediate products of lipid peroxidation. Elevated oxidative stress and ROS levels triggered by MIA led to the activation of the intrinsic pathway through the depolarization of membrane potential, the promotion of the discharge of cytochrome c, and the activation of caspase-3 [10,60,61].…”

The Gut Microbiota of Rats under Experimental Osteoarthritis and Administration of Chondroitin Sulfate and Probiotic

“…Probiotic (PB) groups were administered an oral solution of "Multiprobiotic Simbiter®" (Company "О.D.Prolisok", Ukraine) -a biomass of living cells from the symbiosis of 17 strains of microorganisms physiological for mammalian intestines belonging to 11 species. Most strains were taken from All- The choice of this probiotic is due to its effective multicomponent live bacteria composition and previously shown by us anti-inflammatory and antioxidant properties for this pathology [10,11].…”

Oxidative/antioxidant balance and matrix metalloproteinases level in the knee cartilage of rats under experimental osteoarthritis and probiotic administration

“…Matrix metalloproteinases (MMPs) affect the metabolism of extracellular matrix components and concerned as markers of joint tissue destruction [13,14]. In our previous studies, we found the effect of multistrain PB on the system disruption of free radical processes and levels of MMPs in the bloodstream during experimental OA [15,16], however, the local biochemical mechanisms of these processes were not shown in the cartilage yet. Therefore, the aim of this study to investigate the effect of a multistrain live probiotic on the oxidative-antioxidant balance and activity of MMPs in the cartilage of rats under conditions of experimental osteoarthritis.…”