Glutathione-Stabilized Silver Nanoparticles: Antibacterial Activity against Periodontal Bacteria, and Cytotoxicity and Inflammatory Response in Oral Cells

Zorraquín‐Peña, Irene; Cueva, Carolina; Llano, Dolores González de; Bartolomé, Begoña; Moreno‐Arribas, M. Victoria

doi:10.3390/biomedicines8100375

Cited by 19 publications

(15 citation statements)

References 67 publications

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“… Trigger inflammatory response in human gingival fibroblasts by the increase of cytokine production. [ 58 , 59 ] Abbreviations : Ag NPs, silver nanoparticles; BiOBr NPs, polyethylenimine grafted bismuth oxybromide nanoplates with Fe 3+ ; C1-PNPs, pyridinium amphophile-loaded PLGA (poly (lactic-co-glycolic acid) nanoparticles; CNFs, carbon nanofibers; CS@MoS 2 , chitosan-assisted MoS 2 ; CuS NPs, copper sulfide nanoparticles; dvPtNPs, dualvalent platinum nanoparticles; GNR@LDH-PEG, poly(ethylene glycol) (PEG) modified core-shell GNR@ (gold nanorod) layered double hydroxide nanoparticles; GNSs, gold nanospheres; GO, graphene oxide; GSH, glutathione; GSH-Ag NPs, glutathione-stabilized silver nanoparticles; MDR, multidrug-resistant; MIC, minimum inhibitory concentration; Mg(OH) 2 NPs, magnesium hydroxide nanoparticles; MnO 2 NPs, manganese oxide nanoparticles; MoS 2 /PDA-RGD, molybdenum disulfide/polydopamine-arginine-glycine-aspartic acid; MRSA , methicillin-resistant Staphylococcus aureus ; NCQDs, nitrogen-doped carbon quantum dots; NIR, Near-infrared irradiation; NPs, nanoparticles; PCE, tetrachloroethene; PDA-PEG-Van NPs, polydopamine-based nanoparticles modified with PEG and vancomycin; rGO-Au NPs, reduced-graphene-oxide functionalized with gold nanoparticles; ROS, Reactive Oxygen Species; Tri-Ag NPs, citrate-coated triangular nanoparticles; Tv-Ag NPs, toxicodendron vernicifluum silver nanoparticles. …”

Section: The Cancer Burden In the Worldmentioning

confidence: 99%

Current Trends and Challenges in Pharmacoeconomic Aspects of Nanocarriers as Drug Delivery Systems for Cancer Treatment

Milewska¹,

Niemirowicz-Laskowska²,

Siemiaszko³

et al. 2021

IJN

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Nanotherapy is a part of nanomedicine that involves nanoparticles as carriers to deliver drugs to target locations. This novel targeting approach has been found to resolve various problems, especially those associated with cancer treatment. In nanotherapy, the carrier plays a crucial role in handling many of the existing challenges, including drug protection before early-stage degradations of active substances, allowing them to reach targeted cells and overcome cell resistance mechanisms. The present review comprises the following sections: the first part presents the introduction of pharmacoeconomics as a branch of healthcare economics, the second part covers various beneficial aspects of the use of nanocarriers for in vitro, in vivo, and pre- and clinical studies, as well as discussion on drug resistance problem and present solutions to overcome it. In the third part, progress in drug manufacturing and optimization of the process of nanoparticle synthesis were discussed. Finally, pharmacokinetic and toxicological properties of nanoformulations due to up-to-date studies were summarized. In this review, the most recent developments in the field of nanotechnology’s economic impact, particularly beneficial applications in medicine were presented. Primarily focus on cancer treatment, but also discussion on other fields of application, which are strongly associated with cancer epidemiology and treatment, was made. In addition, the current limitations of nanomedicine and its huge potential to improve and develop the health care system were presented.

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Section: The Cancer Burden In the Worldmentioning

confidence: 99%

Current Trends and Challenges in Pharmacoeconomic Aspects of Nanocarriers as Drug Delivery Systems for Cancer Treatment

Milewska¹,

Niemirowicz-Laskowska²,

Siemiaszko³

et al. 2021

IJN

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“…Silver nanoparticles (AgNPs) have been studied in greater depth than CNFs. They are also cost-effective and possess excellent antimicrobial properties [ 20 , 21 , 22 , 23 , 24 ]. In fact, AgNPs are already broadly used in wound dressings for healing processes and treating burns in biomedicine as well as in the food and textile industries and in paints, household products, catheters, implants, and cosmetics and in combination with many types of materials to prevent infection [ 25 , 26 , 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanofibers versus Silver Nanoparticles: Time-Dependent Cytotoxicity, Proliferation, and Gene Expression

et al. 2021

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Carbon nanofibers (CNFs) are one-dimensional nanomaterials with excellent physical and broad-spectrum antimicrobial properties characterized by a low risk of antimicrobial resistance. Silver nanoparticles (AgNPs) are antimicrobial metallic nanomaterials already used in a broad range of industrial applications. In the present study these two nanomaterials were characterized by Raman spectroscopy, transmission electron microscopy, zeta potential, and dynamic light scattering, and their biological properties were compared in terms of cytotoxicity, proliferation, and gene expression in human keratinocyte HaCaT cells. The results showed that both AgNPs and CNFs present similar time-dependent cytotoxicity (EC50 of 608.1 µg/mL for CNFs and 581.9 µg/mL for AgNPs at 24 h) and similar proliferative HaCaT cell activity. However, both nanomaterials showed very different results in the expression of thirteen genes (superoxide dismutase 1 (SOD1), catalase (CAT), matrix metallopeptidase 1 (MMP1), transforming growth factor beta 1 (TGFB1), glutathione peroxidase 1 (GPX1), fibronectin 1 (FN1), hyaluronan synthase 2 (HAS2), laminin subunit beta 1 (LAMB1), lumican (LUM), cadherin 1 CDH1, collagen type IV alpha (COL4A1), fibrillin (FBN), and versican (VCAN)) treated with the lowest non-cytotoxic concentrations in the HaCaT cells after 24 h. The AgNPs were capable of up-regulating only two genes (SOD1 and MMP1) while the CNFs were very effective in up-regulating eight genes (FN1, MMP1, CAT, CDH1, COL4A1, FBN, GPX1, and TGFB1) involved in the defense mechanisms against oxidative stress and maintaining and repairing tissues by regulating cell adhesion, migration, proliferation, differentiation, growth, morphogenesis, and tissue development. These results demonstrate CNF nanomaterials’ unique great potential in biomedical applications such as tissue engineering and wound healing.

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“… 86 , 87 Similarly, glutathione-stabilized AgNPs may increase the production of IL-6, IL-8 and TNFα in HGF-1 cells. 55 TNFα expression may also be increased when RAW264.7 cells are treated with 68.9 nm AgNPs. 88 Interestingly, a recent study examined the role of highly biocompatible biomimetic scaffold embedding AgNPs in the treatment of oral lesions.…”

Section: Discussionmentioning

confidence: 98%

Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery Platforms: Their Potential Use in Treating Periodontitis

Steckiewicz¹,

Cieciórski²,

Barcińska³

et al. 2022

IJN

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Purpose Periodontal disease (PD), defined as oral inflammation caused by dental plaque, is an emerging problem. PD may lead to tooth loss, and treatment options are limited. In this study, we designed, synthesized, and characterized silver nanoparticles (AgNPs) conjugated with chlorhexidine (AgNPs-CHL) or metronidazole (AgNPs-PEG-MET) to determine whether they can be used to treat PDs. Materials and Methods AgNPs were synthesized and characterized by transmission electron microscopy, UV-vis spectrometry, thermogravimetric analyses, and dynamic light scattering. We determined the safety and the antimicrobial and anti-inflammatory properties of synthesized AgNPs in an in vitro model of periodontitis. Antimicrobial properties were determined by measuring the minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) on reference strains of bacteria and fungi. Human gingival fibroblast (HGF-1), murine macrophage (RAW264.7) and human foetal osteoblast (hFOB1.19) cells were used in the study. Lipopolysaccharide (LPS) was used to induce inflammation. Cytokine levels were measured using an enzyme-linked immunosorbent assay; metalloproteinase expression was measured using Western blotting. Results The synthesized AgNPs were spherical and narrow-dispersed with an average diameter of 13.4 nm ± 3.0 nm in the case of AgNPs-CHL and 3.72 nm ± 0.72 nm in the case of AgNPs-PEG-MET. Both types of AgNPs were active against bacteria and fungi. AgNPs-CHL proved to be a more potent antimicrobial agent, although they were more cytotoxic than AgNPs-PEG-MET; however, both demonstrated beneficial properties in nontoxic concentrations. AgNPs-CHL and AgNPs-PEG-MET decreased the production of proinflammatory cytokines IL-1β, IL-6, IL-8 and TNFα. Both agents also decreased the levels of metalloproteinases MMP3 and MMP8, which may indicate that they will inhibit tissue degradation. Conclusion AgNPs-CHL and AgNPs-PEG-MET may be possible therapeutic options for PD, as they have antibacterial and anti-inflammatory properties. However, to fully understand the potential of AgNPs, our in vitro findings must be evaluated in an in vivo model.

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Glutathione-Stabilized Silver Nanoparticles: Antibacterial Activity against Periodontal Bacteria, and Cytotoxicity and Inflammatory Response in Oral Cells

Cited by 19 publications

References 67 publications

Current Trends and Challenges in Pharmacoeconomic Aspects of Nanocarriers as Drug Delivery Systems for Cancer Treatment

Current Trends and Challenges in Pharmacoeconomic Aspects of Nanocarriers as Drug Delivery Systems for Cancer Treatment

Carbon Nanofibers versus Silver Nanoparticles: Time-Dependent Cytotoxicity, Proliferation, and Gene Expression

Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery Platforms: Their Potential Use in Treating Periodontitis

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