Carbon Nanofibers versus Silver Nanoparticles: Time-Dependent Cytotoxicity, Proliferation, and Gene Expression

Salesa, Beatriz; Assis, Marcelo; Andrés, Juán; Serrano‐Aroca, Ãngel

doi:10.3390/biomedicines9091155

Cited by 23 publications

(23 citation statements)

References 65 publications

(82 reference statements)

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“…In that study, multi-layer GO showed to be able to up-regulate four genes (CAT, TGFB1, FN1, and CDH1) that are relevant in biomedicine [13]. Another CBN in the form of carbon filamentous hollow materials has recently been shown to be capable of up-regulating many more genes than other types of nanomaterials, silver nanoparticles, and thus showing the great potential of CBNs in the biomedical field [14].…”

Section: Introductionmentioning

confidence: 93%

“…Therefore, these results reinforce the idea of using these nanomaterials for biomedical applications such as wound healing and skin tissue engineering. Nonetheless, it is important to mention that other carbon materials such as 1D filamentous hollow carbon nanofibers showed effective results in up-regulating eight (FN1, MMP1, CAT, CDH1, COL4A1, FBN, GPX1, and TGFB1) of the thirteen analyzed genes [14].…”

Section: Gene Expressionmentioning

confidence: 99%

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Graphene Nanoplatelets: In Vivo and In Vitro Toxicity, Cell Proliferative Activity, and Cell Gene Expression

et al. 2022

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Multi-layer graphene (2–10 layers), also called graphene nanoplatelets (GNPs), is a carbon-based nanomaterial (CBN) type with excellent properties desirable for many biomedical applications. Despite the promising advantages reported of GNPs, nanoscale materials may also present a potential hazard to humans. Therefore, in this study, the in vivo toxicity of these nanomaterials at a wide range of concentrations from 12.5 to 500 µg/mL was evaluated in the Caenorhabditis elegans model for 24 h (acute toxicity) and 72 h (chronic toxicity). Furthermore, their in vitro toxicity (from 0 to 10 µg/mL for 12 and 24 h), proliferative activity at 72 and 96 h, and their effect on the expression of thirteen genes in human keratinocytes HaCaT cells were studied. The physico-chemical and morphological aspects of the GNPs used in this study were analyzed by Raman scattering spectroscopy, electron microscopy, zeta potential as a function of pH, and particle size measurements by dynamic light scattering. The results of this study showed that GNPs showed in vivo non-toxic concentrations of 25 and 12.5 µg/mL for 24 h, and at 12.5 µg/mL for 72 h. Moreover, GNPs present time-dependent cytotoxicity (EC50 of 1.142 µg/mL and 0.760 µg/mL at 12 h and 24 h, respectively) and significant proliferative activity at the non-toxic concentrations of 0.005 and 0.01 μg/mL in the HaCaT cell line. The gene expression study showed that this multi-layer-graphene is capable of up-regulating six of the thirteen genes of human keratinocytes (SOD1, CAT, TGFB1, FN1, CDH1, and FBN), two more genes than other CBNs in their oxidized form such as multi-layer graphene oxide. Therefore, all these results reinforce the promising use of these CBNs in biomedical fields such as wound healing and skin tissue engineering.

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Section: Introductionmentioning

confidence: 93%

Section: Gene Expressionmentioning

confidence: 99%

Graphene Nanoplatelets: In Vivo and In Vitro Toxicity, Cell Proliferative Activity, and Cell Gene Expression

et al. 2022

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“…Ag can be particularly effective when applied in coatings made of nanoparticles [45] and in nanostructures [46] containing large surface-to-bulk ratio particles with considerable quantities of Ag oxide that provide a source of Ag ions to effectively kill bacteria [47]. Ag nanoparticles are used as antimicrobial agents in a broad range of industrial applications including wound dressings, food and textile area, paints, household products, catheters, implants, and cosmetics, among many others [48]. Research has shown that the size of Ag nanoparticles can significantly influence their efficacy as an antiviral agent and the effective upper size limit appears to be around 25 nm [49].…”

Section: Silvermentioning

confidence: 99%

Recent Advances in Metal-Based Antimicrobial Coatings for High-Touch Surfaces

Birkett

Dover

Lukose

et al. 2022

IJMS

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International interest in metal-based antimicrobial coatings to control the spread of bacteria, fungi, and viruses via high contact human touch surfaces are growing at an exponential rate. This interest recently reached an all-time high with the outbreak of the deadly COVID-19 disease, which has already claimed the lives of more than 5 million people worldwide. This global pandemic has highlighted the major role that antimicrobial coatings can play in controlling the spread of deadly viruses such as SARS-CoV-2 and scientists and engineers are now working harder than ever to develop the next generation of antimicrobial materials. This article begins with a review of three discrete microorganism-killing phenomena of contact-killing surfaces, nanoprotrusions, and superhydrophobic surfaces. The antimicrobial properties of metals such as copper (Cu), silver (Ag), and zinc (Zn) are reviewed along with the effects of combining them with titanium dioxide (TiO2) to create a binary or ternary contact-killing surface coatings. The self-cleaning and bacterial resistance of purely structural superhydrophobic surfaces and the potential of physical surface nanoprotrusions to damage microbial cells are then considered. The article then gives a detailed discussion on recent advances in attempting to combine these individual phenomena to create super-antimicrobial metal-based coatings with binary or ternary killing potential against a broad range of microorganisms, including SARS-CoV-2, for high-touch surface applications such as hand rails, door plates, and water fittings on public transport and in healthcare, care home and leisure settings as well as personal protective equipment commonly used in hospitals and in the current COVID-19 pandemic.

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“…Proteins spontaneously and quickly adsorb onto carbon nanoparticles, forming the so-called protein corona and covering the surface [69]. The organic components of GAM broth contain a Therefore, the results of this study show that the incorporation of a low amount (1% w/w) of filamentous 1D hydrophobic CNFs, which are longer nanoparticles and possess lower negative charge than the GO nanosheets [63][64][65][66], significantly increased the degradability of the hydrophobic PHBV after 3 months in simulated intestinal media (both acid aqueous and GAM media). The long filamentous hydrophobic CNFs incorporated into the PHBV polymer matrix creates carbon-based nanochannels through which water (in this case aqueous medium) can penetrate at ultrafast speed according to previous studies [70][71][72], increasing the total area of PHBV chains exposed to degradation.…”

Section: Degradation In Simulated Intestinal Conditionsmentioning

confidence: 79%

“…The dynamic light scattering (DLS) technique showed a particle hydrodynamic size of the GO nanosheets that ranged from 300 to 500 nm, depending on the nanofluid (polyethylene glycol, 1-octadecanothiol, or Triton X-100 surfactant) used for these measurements [65]. However, the DLS hydro-dynamic sizes of the 1D nanomaterial showed to be much larger, ranging from 811.2 to 1142 nm, depending also on the nanofluid used (DMEM or water, respectively) [66].…”

Section: Methodsmentioning

confidence: 99%

Graphene Oxide versus Carbon Nanofibers in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Films: Degradation in Simulated Intestinal Environments

et al. 2022

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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a microbial biodegradable polymer with a broad range of promising industrial applications. The effect of incorporation of low amounts (1% w/w) of carbon nanomaterials (CBNs) such as 1D carbon nanofibers (CNFs) or 2D graphene oxide (GO) nanosheets into the PHBV polymer matrix affects its degradation properties, as it is reported here for the first time. The study was performed in simulated gut conditions using two different media: an acidic aqueous medium (pH 6) and Gifu anaerobic medium. The results of this study showed that the incorporation of low amounts of filamentous 1D hydrophobic CNFs significantly increased the degradability of the hydrophobic PHBV after 3 months in simulated intestinal conditions as confirmed by weight loss (~20.5% w/w in acidic medium) and electron microscopy. We can attribute these results to the fact that the long hydrophobic carbon nanochannels created in the PHBV matrix with the incorporation of the CNFs allowed the degradation medium to penetrate at ultrafast diffusion speed increasing the area exposed to degradation. However, the hydrogen bonds formed between the 2D hydrophilic GO nanosheets and the hydrophobic PHBV polymer chains produced a homogeneous composite structure that exhibits lower degradation (weight loss of ~4.5% w/w after three months in acidic aqueous medium). Moreover, the water molecules present in both degradation media can be linked to the hydroxyl (-OH) and carboxyl (-COOH) groups present on the basal planes and at the edges of the GO nanosheets, reducing their degradation potential.

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Carbon Nanofibers versus Silver Nanoparticles: Time-Dependent Cytotoxicity, Proliferation, and Gene Expression

Cited by 23 publications

References 65 publications

Graphene Nanoplatelets: In Vivo and In Vitro Toxicity, Cell Proliferative Activity, and Cell Gene Expression

Graphene Nanoplatelets: In Vivo and In Vitro Toxicity, Cell Proliferative Activity, and Cell Gene Expression

Recent Advances in Metal-Based Antimicrobial Coatings for High-Touch Surfaces

Graphene Oxide versus Carbon Nanofibers in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Films: Degradation in Simulated Intestinal Environments

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