Glutathione S-transferases activity in patients with colorectal cancer

Nomani, Hamid; Ghobadloo, Shahrokh M.; Yaghmaei, Bahram; Rezvanie, Nayeb Ali; Yaghmaei, Kaveh

doi:10.1016/j.clinbiochem.2005.04.004

Cited by 15 publications

(8 citation statements)

References 16 publications

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“…In the relevant animal models described in our study, we found that alkaline phosphatase activity was significantly reduced by 5-FU, but not significantly affected after the glutamine supplementation. In contrast, glutathione S-transferase, glutathione reductase and glutathione peroxidase, showed the increase in enzyme activity after 5-FU, which is in accordance with the findings of other authors that these enzymes are involved in the protection from chemically induced intestinal damage (16,17). The significant dose-dependent decrease in ALP activity in intestinal mucosa may be the result of two phenomena: the downregulation of the expression of ALP enzyme and the inhibition of DNA synthesis, showed by the decline in DNA content.…”

Section: Discussionsupporting

confidence: 92%

Intestinal alkaline phosphatase activity as a molecular marker of enterotoxicity induced by single dose of 5-fluorouracil and protective role of orally administered glutamine

et al. 2006

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Background. One of the critical limitations for the administration of the chemotherapy is the toxicity affecting normal tissue. The main target organs for 5-fluorouracil (5-FU) toxicity in humans and experimental animals are the gastrointestinal tract, bone marrow, and skin. The cytotoxic effects of antimetabolite chemotherapy are based on their role as substrates for the same transport processes and enzymes involved in anabolism and catabolism as the natural substrates. The main goal of our study was to analyze the dose-dependent antiproliferative effects of 5-FU on intestinal mucosa, enterotoxic potential of 5-FU in experimental animals and to test possible protective role of glutamine. Methods. In our study, we used Sprague Dawley rats. The control group of rats included 50 animals, while the groups where either 5-fluorouracil (5-FU) alone or 5-FU and glutamine were administered included 200 animals. All experimental animals were further stratified according to the experimental model (25 animals in each of 8 experimental subgroups of animals). The 5-FU was administered by intraperitoneal application in single dose of 0, 100, 200, 300, and 400 mg of 5-FU per kg of body weight. Water solution of 1% glutamine was prepared daily and administered orally, in volume of 200 ml, for 7 days continuously, after the 7th day of 5-FU administration. Experimental animals were sacrificed 7 days after the administration of 5-FU. The isolation of enterocytes was performed according to the method of Kralovansky et al. In cell homogenate obtained by described method, we determined the protein content using the Biuret method and the DNA content using the Burton reagent. The activities of enzymes alkaline phosphatase (ALP), glutathione S-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPX) were determined by kinetic method. All paraffin samples of the small intestine were stained by haematoxiline and eosine(HE method). All the experiments were done in duplicate and analyzed by standard statistical methods. All the experiments were done in duplicate and analyzed by standard statistical methods. Results: Our results of enterotoxicity induced by intraperitonealy administered 5-FU showed statistically significant decrease of DNA content in small intestine samples of experimental animals, decrease in activity of intestinal alkaline phosphatase enzyme and the increase in glutathione-dependent enzymes. The glutamine supplementation reduced 5-FU intestinal toxicity. Conclusion: Intestinal alkaline phosphatase is a good marker of the dose-dependent enterotoxicity induced by 5-fluorouracil

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Section: Discussionsupporting

confidence: 92%

Intestinal alkaline phosphatase activity as a molecular marker of enterotoxicity induced by single dose of 5-fluorouracil and protective role of orally administered glutamine

et al. 2006

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“…A potential effect of the GSTP1 Ile105Val polymorphism genotype on smoking and the risk of prostate cancer was reported by Mao et al [42] . Nomani et al [43] suggested GST measurement as a marker in colorectal cancer, as they found plasma GSTs activity significantly higher in colorectal cancer patients than those obtained from normal individuals. According to Prabhu and Bhat [44] , alterations in serum total GST level may have a role in cancer progression.…”

Section: Expression Of Glutathione S-transferase In Cancermentioning

confidence: 98%

Antioxidant enzymes and cancer

Khan

Tania

Zhang

et al. 2010

Chin. J. Cancer Res.

150

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Although oxidation is the most common biological and energy producing reaction, oxidative stress is harmful to cell, because the products of oxidation such as free radicals and peroxides damage the cellular components, causing several diseases. Damage in DNA is responsible for cancer formation and progression. However, several enzymes such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase etc. act as antioxidants to influence oxidative stress. Polymorphisms in these enzymes are supposed to be associated with DNA damage and subsequently the individual's risk of cancer susceptibility. This review article aims to further elucidate the relationship between antioxidant enzymes and cancers by summarizing the findings of some of the important study concerning expression levels and genetic polymorphisms of antioxidant enzymes in cancer patients.

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“…There was a significant GSTs over activity in tumor compared with normal margins. This strong correlation supported the plasma GST activity as a non-invasive biomarker [14]. GSTM1, P1, T1, and CYP2E1 polymorphisms were also assessed among Iranian CRC subjects.…”

Section: Main Textmentioning

confidence: 55%

Genetic and molecular origins of colorectal Cancer among the Iranians: an update

Abbaszadegan

Moghbeli

2018

Diagn Pathol

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BackgroundColorectal cancer (CRC) is one the leading causes of cancer related deaths among Iranians. Despite the various progresses in new therapeutic methods, it has still a low rate of survival. This high ratio of mortality is mainly related to the late diagnosis, in which the patients refer for treatment in advanced stages of tumor.Main bodycolorectal cancer progression is largely associated with molecular and genetic bases. Although Iran has a high ratio of CRC mortality, there is not an efficient genetic panel for detection and prognosis. Therefore, it is critical to introduce new diagnostic markers with ability to detect in early stages.ConclusionPresent review summarizes all of the genetic and epigenetic factors which are reported in CRC until now among the Iranian patients to pave the way of incorporation of new ethnic specific markers into the clinical practice and development of new targeted therapeutic methods.

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Glutathione S-transferases activity in patients with colorectal cancer

Cited by 15 publications

References 16 publications

Intestinal alkaline phosphatase activity as a molecular marker of enterotoxicity induced by single dose of 5-fluorouracil and protective role of orally administered glutamine

Intestinal alkaline phosphatase activity as a molecular marker of enterotoxicity induced by single dose of 5-fluorouracil and protective role of orally administered glutamine

Antioxidant enzymes and cancer

Genetic and molecular origins of colorectal Cancer among the Iranians: an update

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