Glutathione S-Transferase Expression in Upper Urinary Tract Urothelial Carcinomas: a Taiwan Study

Abstract

Objectives: Glutathione S-transferase (GST) isoenzymes play important roles in resistance to cell apoptosis and carcinogenesis. We aimed to establish the relationship between GST expression and the prognosis of upper urinary tract urothelial carcinoma (UTT-UC) in Taiwan. Methods: This study retrospectively reviewed 46 patients with pathologically confirmed UUT-UC at Kaohsiung Medical University Hospital. In each patient, expression of GSTT1 and GSTP1 was compared between urothelial carcinoma and normal urothelial cells by Western blotting. Results: GSTP1 expression in the UUT-UC cells was significantly higher than that in normal urothelial cells (1.6 fold, p<0.001). Expression of GSTT1 was significantly associated with the invasiveness of the carcinoma (p=0.006). Conclusions: In UUT-UC, GSTP1 might be a potential tumor marker, whereas high GSTT1 expression could be used as an indicator of cancer progression. This study is the first to demonstrate potential applications of different GST isoenzymes for biomolecular analysis of UUT-UCs in Taiwan.

Evaluation of glutathione S‐transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer

“…In comparison to our findings, several studies have reported an upregulated GSTP1 expression which increased gradually with high-grade tumor or invasive-stage and was linked to decreased levels of urothelial cells apoptosis (Chen et al, 2013;Pljesa-Ercegovac et al, 2011;Savic-Radojevic et al, 2007).…”

“…Some studies indicated an overexpression of GSTP1 gene in esophageal cancer (Joshi et al., ), colorectal cancer (Zhang et al., ), renal cancer (Kaprilian et al., ), lung cancer (Yang et al., ), and in BC (Chen et al., ; Pljesa‐Ercegovac et al., ; Savic‐Radojevic et al., ). Other studies showed reduced GSTP1 gene expression in prostate, endometrial, hepatocellular, and ovarian cancers (Chan et al., ; Li et al., ; Lin et al., ; Martignano et al., ; Mian et al., ; Shilpa et al., ; Zelic et al., ; Zhang et al., ).…”

“…, lung cancer (Yang et al, 2006), and in BC (Chen et al, 2013;Pljesa-Ercegovac et al, 2011;Savic-Radojevic et al, 2007). Other studies showed reduced GSTP1 gene expression in prostate, endometrial, hepatocellular, and ovarian cancers (Chan et al, 2005;Li et al, 2015;Lin et al, 2001;Martignano et al, 2016;Mian et al, 2016;Shilpa et al, 2014;Zelic et al, 2016;Zhang et al, 2015).…”

MCM10 overexpression implicates adverse prognosis in urothelial carcinoma

“…Upper urinary tract urothelial carcinoma (UTUC) comprises only 5% of all urothelial tumors in Western countries [1], but its incidence is high in Taiwan, with a ratio 3.08:6.72, UTUC to urinary bladder urothelial carcinoma (UBUC) [2]. Despite advances in surgical technique and improved understanding of UC carcinogenesis [3–7], 5-year survival rates for patients remain suboptimal [8, 9].…”

GSTT1 is Deregulated in Left Colon Tumors

“…In addition, deregulated GSTT1 expression has been observed in some cancers including bladder cancer, breast cancer and glioma (Dialyna et al, 2001;Diedrich et al, 2006;Ha et al, 2011;Chen et al, 2013). A previous study comparing GSTT1 protein levels in paired normal tissue and colorectal and gastric tumors has found a mild decrease in GSTT1 protein levels (de Bruin et al, 1999).…”