Glutathione-S-Transferase and GST-π in Gastric Carcinoma

Mandal, Dr Subhasis

doi:10.18535/jmscr/v5i7.233

Cited by 2 publications

(1 citation statement)

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“…Likewise, in their study of patients with gastrointestinal system tumors, Scibior et al [22]. Mandal et al, [23], stated that GST activity in cancerous gastric and colon tissues was statistically significantly higher than in the control group. In our study, the erythrocyte GST activity of both individuals with colon cancer and gastric cancer was significantly higher than the GST values of control individuals (p<0.05) (Table 2).…”

Section: Discussionmentioning

confidence: 96%

Determination of erythrocyte Glutathione S-Transferase activity in individuals with gastric and colon cancer

Kemal¹,

Dağ²,

Özpınar³

2022

GSC Biol. Pharm. Sci.

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Glutathione-S-Transferase (GST) in human cells has great importance in the detoxification mechanism of carcinogenic chemicals. Therefore, (GST) may be a useful tumor marker. This study examines whether GST activity in Gastric cancer (GC) and Colon Cancer (CC) is a helpful marker in diagnosing and monitoring the disease course. GST activity was investigated in patients with CC and GC and healthy individuals. Erythrocyte isolation was performed in 3 ml blood samples from volunteers aged 18-75 years. Hemoglobin amounts were determined from the standard graph drawn by monitoring the conversion of methemoglobin to cyanmethemoglobin in the presence of cyanide at 540 nm. Glutathione S-transferase activity was determined by measuring the amount of enzyme that catalyzes 1 µmol of S-(2,4-dinitrophenyl) glutathione formed per minute using 1-Chloro-2,4-Dinitrobenzene. The mean values of GST activities of patients with CC and GC, respectively; (1.28 ± 0.23 U/gHb; 1.20 ± 0.30 U/gHb), were significantly higher when compared to the mean values of healthy individuals with GST activity (0.59 ± 0.13 U/gHb) (p < 0.05). The GST activity of patients with colon cancer, measured as (1.56 ± 0.13 U/gHb) after chemotherapy, was significantly higher than before (1.09 ± 0.12 U/gHb) (p < 0.05). GST activity measured as (1.53± 0.24 U/gHb) after chemotherapy in gastric cancer patients was significantly higher when compared to the value measured before chemotherapy (0.97± 0.12 U/gHb) (p < 0.05). Our results show that the change in GST activity in CC and GC can be used as a biomarker to monitor the disease course and response to chemotherapy.

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Section: Discussionmentioning

confidence: 96%