Glutathione peroxidase-like nanozymes: mechanism, classification, and bioapplication

Lai, Yifan; Wang, Jingyu; Ning, Yuping; Zhang, Qiaochu; Wu, Jiangjiexing; Qi, Wei; Su, Rongxin

doi:10.1039/d2bm01915a

Cited by 20 publications

(12 citation statements)

References 127 publications

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“…31 Subsequently, it was observed that V 2 O 5 nanowires also catalyze the glutathione peroxidase-dependent conversion of GSH (reduced glutathione) to glutathione disulfide. 32 Further research has also evidenced that graphene oxide (GO) and single-walled carbon nanotubes (SWCNs) are also capable of imitating HRP. 33 These carbon-based nanomaterials are shown to catalyze the oxidation reaction of TMB and H 2 O 2 to yield a blue-colored product at pH 4, which resembles the working condition of the HRP enzyme.…”

Section: ■ Nanozymes: Structure and Functionsmentioning

confidence: 99%

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Recent Advancements in the Formulation of Nanomaterials-Based Nanozymes, Their Catalytic Activity, and Biomedical Applications

Singh,

Rai,

Tiwari

et al. 2023

ACS Appl. Bio Mater.

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Nanozymes are nanoparticles with intrinsic enzyme-mimicking properties that have become more prevalent because of their ability to outperform conventional enzymes by overcoming their drawbacks related to stability, cost, and storage. Nanozymes have the potential to manipulate active sites of natural enzymes, which is why they are considered promising candidates to function as enzyme mimetics. Several microscopy- and spectroscopy-based techniques have been used for the characterization of nanozymes. To date, a wide range of nanozymes, including catalase, oxidase, peroxidase, and superoxide dismutase, have been designed to effectively mimic natural enzymes. The activity of nanozymes can be controlled by regulating the structural and morphological aspects of the nanozymes. Nanozymes have multifaceted benefits, which is why they are exploited on a large scale for their application in the biomedical sector. The versatility of nanozymes aids in monitoring and treating cancer, other neurodegenerative diseases, and metabolic disorders. Due to the compelling advantages of nanozymes, significant research advancements have been made in this area. Although a wide range of nanozymes act as potent mimetics of natural enzymes, their activity and specificities are suboptimal, and there is still room for their diversification for analytical purposes. Designing diverse nanozyme systems that are sensitive to one or more substrates through specialized techniques has been the subject of an in-depth study. Hence, we believe that stimuli-responsive nanozymes may open avenues for diagnosis and treatment by fusing the catalytic activity and intrinsic nanomaterial properties of nanozyme systems.

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Section: ■ Nanozymes: Structure and Functionsmentioning

confidence: 99%

“…In the presence of H 2 O 2 , V 2 O 5 (vanadium pentoxide) nanowires can mimic V-HPOs and convert bromide into water and HOBr . Subsequently, it was observed that V 2 O 5 nanowires also catalyze the glutathione peroxidase-dependent conversion of GSH (reduced glutathione) to glutathione disulfide …”

Section: Mode Of Actionmentioning

confidence: 99%

Recent Advancements in the Formulation of Nanomaterials-Based Nanozymes, Their Catalytic Activity, and Biomedical Applications

Singh,

Rai,

Tiwari

et al. 2023

ACS Appl. Bio Mater.

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“…63,64 Selenium was found to be an important component in GPx; therefore, researchers initially explored GPx mimetic enzymes pertaining to selenium-based compounds. 64 It catalyzes the change of GSH (reduced glutathione) to GSSG (oxidized glutathione). Li et al 65 reported an ultrasmall bimetallic oxide nanozyme (CuFe 2 O 4 −PEG) that exhibited GPx-like activity in addition to POD-and CAT-like activities.…”

Section: Peroxide-like Activitymentioning

confidence: 99%

“…Glutathione peroxidase (GPx) is an antioxidant enzyme responsible for maintaining cellular redox homeostasis. , Selenium was found to be an important component in GPx; therefore, researchers initially explored GPx mimetic enzymes pertaining to selenium-based compounds . It catalyzes the change of GSH (reduced glutathione) to GSSG (oxidized glutathione).…”

Section: Nanozymementioning

confidence: 99%

“…Nanozymes can use the tumor microenvironment to activate the Fenton reaction (Fenton-like reaction) to produce strongly oxidizing • OH for tumor-specific therapy . Nanozymes can also be used to enhance • OH production by in situ modulation of TME, such as down-regulation of pH or production of H 2 O 2 to weaken tumor defense against reactive oxygen species by the depletion of GSH , and to achieve tumor starvation therapy by the depletion of glucose. , Furthermore, TME-responsive nanomaterials can be designed for the acidic environment of TME, which only targets tumor tissues and does not affect normal human tissue cells.…”

Section: Microenvironmentmentioning

confidence: 99%

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Nanozyme-Based Remodeling of Disease Microenvironments for Disease Prevention and Treatment: A Review

Liu

et al. 2023

ACS Appl. Nano Mater.

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Nanomaterials that can mimic the activity of various enzymes have been studied extensively in the biomedical field in recent years. Nanozymes offer better stability and economic value in comparison to natural enzymes. Additionally, nanozymes have characteristics of nanomaterials, such as photothermal properties and designability, and therefore have considerable potential. The cellular microenvironment provides the basis for maintaining normal cell growth and function. Increasing evidence shows that imbalances in the homeostasis of the cellular microenvironment constitute a major factor in the development of many diseases. Nanozymes have received considerable attention in recent years as regulators of the cellular microenvironment. In this Review, research on the use of nanozymes to remodel the disease microenvironment is discussed. First, the definition, classification, and enzyme-like activities of nanozymes are introduced; several major disease microenvironments are summarized including tumor, inflammatory, and trauma microenvironments. The role of nanozymes in disease microenvironments and the main strategies for constructing intelligent and responsive nanozyme systems are discussed. The specific applications of nanozymes in remodeling the diseased microenvironment are highlighted. Finally, the therapeutic strategies of nanozymes to remodel the disease microenvironment are summarized and prospected. Nanozyme disease microenvironment remodeling is expected to become a major therapeutic strategy or an adjunct for the prevention and treatment of related diseases.

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A Glutathione Peroxidase‐Mimicking Nanozyme Precisely Alleviates Reactive Oxygen Species and Promotes Periodontal Bone Regeneration

Zhu,

Wu,

et al. 2023

Adv Healthcare Materials

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The use of oxidoreductase nanozymes to regulate reactive oxygen species (ROS) has gradually emerged in periodontology treatments. However, current nanozymes for treating periodontitis eliminate ROS extensively and non‐specifically, ignoring the physiological functions of ROS under normal conditions, which may result in uncontrolled side effects. Herein, using the MIL‐47(V)‐F (MVF) nanozyme, which mimics the function of glutathione peroxidase (GPx), it is proposed that ROS can be properly regulated by specifically eliminating H2O2, the most prominent ROS. Through H2O2 elimination, MVF contributes to limiting inflammation, regulating immune microenvironment, and promoting periodontal regeneration. Moreover, MVF stimulates osteogenic differentiation of periodontal stem cells directly, further promoting regeneration due to the vanadium in MVF. Mechanistically, MVF regulates ROS by activating the nuclear factor erythroid 2‐related factor 2/heme oxygenase 1 (Nrf2/HO‐1) pathway and promotes osteogenic differentiation directly through the phosphatidylinositol 3‐kinase/protein kinase B (PI3K/Akt) pathway. A promising periodontitis therapy strategy is presented using GPx‐mimicking nanozymes through their triple effects of antioxidation, immunomodulation, and bone remodeling regulation, making nanozymes an excellent tool for developing precision medicine.

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Glutathione peroxidase-like nanozymes: mechanism, classification, and bioapplication

Abstract: The field of nanozymes is developing rapidly. In particular, glutathione peroxidase (GPx)-like nanozymes, which catalytically reduce H2O2/organic hydroperoxides to H2O/alcohols, have generated considerable attention. GPx-like nanozymes are powerful antioxidant enzymes...

Cited by 20 publications

References 127 publications

Recent Advancements in the Formulation of Nanomaterials-Based Nanozymes, Their Catalytic Activity, and Biomedical Applications

Recent Advancements in the Formulation of Nanomaterials-Based Nanozymes, Their Catalytic Activity, and Biomedical Applications

Nanozyme-Based Remodeling of Disease Microenvironments for Disease Prevention and Treatment: A Review

A Glutathione Peroxidase‐Mimicking Nanozyme Precisely Alleviates Reactive Oxygen Species and Promotes Periodontal Bone Regeneration

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