Glutathione peroxidase activity in selenium-deficient rat liver

Lawrence, Richard; Burk, Raymond F.

doi:10.1016/0006-291x(76)90747-6

Cited by 3,137 publications

(1,230 citation statements)

References 12 publications

Supporting

Mentioning

1,207

Contrasting

Unclassified

Order By: Relevance

“…The assay of Catalase (CAT) was performed by method of Aebi (15,16). Glutathione peroxides (GPX) and Glutathione-S-transferase (GST) activities were estimated by the method of Lawrence and Gupta (17,18). Lipid peroxidase (LPO) was determined by the method using the thiobarbituric acid reaction (19).…”

Section: Assay Of Antioxidant Enzymesmentioning

confidence: 99%

Effect ofOcimum sanctum (Tulsi) and vitamin E on biochemical parameters and retinopathy in streptozotocin induced diabetic rats

Halim

Mukhopadhyay

2006

Indian J Clin Biochem

View full text Add to dashboard Cite

show abstract

Section: Assay Of Antioxidant Enzymesmentioning

confidence: 99%

Effect ofOcimum sanctum (Tulsi) and vitamin E on biochemical parameters and retinopathy in streptozotocin induced diabetic rats

Halim

Mukhopadhyay

2006

Indian J Clin Biochem

View full text Add to dashboard Cite

show abstract

“…SOD activity of liver and heart was measured using xanthine and xanthine oxidases to generate superoxide radicals which react with nitroblue tetrazolium (NBT) by the methods of Sun et al [23] . The GSH-Px activity was determined according to Lawrence and Burk [24] . The GSH content of the liver and heart was measured at 412 nm by the method of Sedlak and Lindsay [25] .…”

Section: Methodsmentioning

confidence: 99%

Soğuk Stresine Maruz Bırakılan Japon Bıldırcınlarında Diyete İlave Edilen Çöven Ekstraktının Yumurtlama Performansı, Kan Biyokimyasal Parametreleri, Göğüs Eti Yağ Asidi Profili İle Karaciğer ve Kalp Dokusu Antioksidatif Potansiyeli Üzerine Etkisi

Çiftçi¹,

Dalkiliç²,

Şimşek³

et al. 2016

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

The purpose of this study was to evaluate the effects of dietary Soapwort Extract (SE) supplementation on laying performance, egg quality, some offal weights, some serum parameters, antioxidant status of liver and heart tissues and fatty acid composition of breast meat in chronic cold stressed Japanese quails. A total of sixty three 45-day-old Japanese quails were divided into three groups with three replicate. One group was fed with the corn-soybean based diet alone (control group) and the others were fed with the basal diet supplemented by 50 and 100 ppm SE. Cold stress was applied every night between 22.00 to 06.00 h as 7±1°C. SE supplementation had no significant effect on laying performance, offal weights and egg quality parameters with the exception of egg and albumen weights. Serum glucose, triglyceride, uric acid and aspartate aminotransferase (AST) levels were reduced in SE-50 group. While supplementation of 100 ppm SE decreased both liver and heart MDA levels, both amounts of SE increased the liver GSH-Px enzyme activity. Supplementation of 100 ppm SE decreased the levels of oleic acid and MUFA in breast meat, and it significantly increased the linoleic, linolenic and arachidonic acid, PUFA, total omega 6 and the ratio of omega 6/omega 3. It is displayed that SE supplementation to diets of laying quails exposed to cold stress can alleviate the detrimental effects of oxidative stress with no affecting on performance parameters. Keywords: Antioxidant, Cold stress, Fatty acid, Laying performance, Saponin, Soapwort extract Soğuk Stresine Maruz Bırakılan Japon Bıldırcınlarında Diyete İlave Edilen Çöven Ekstraktının Yumurtlama Performansı, Kan Biyokimyasal Parametreleri, Göğüs Eti Yağ Asidi Profili İle Karaciğer ve Kalp Dokusu Antioksidatif Potansiyeli Üzerine Etkisi ÖzetBu çalışmada, karma yeme ilave edilen çöven ekstraktının (SE), kronik soğuk stresine maruz bırakılan Japon bıldırcınlarında performans, yumurta kalitesi, bazı organ ağırlıkları, bazı serum parametreleri, karaciğer ve kalp dokularının antioksidan durumları ve göğüs eti yağ asidi profili üzerine etkileri araştırılmıştır. Toplam olarak 63 adet 45 günlük bıldırcın, 3 tekerrürlü 3 gruba ayrılmıştır. Gruplardan biri mısır-soya küspesine dayalı temel yem ile (kontrol grubu), diğer gruplar temel yeme 50 ve 100 ppm SE ilave edilen yemle beslenmişlerdir. Soğuk stresi her akşam 22.00-06.00 saatleri arasında kümes ısısı 7±1°C'ye düşürülerek uygulanmıştır. SE ilavesi performans, bazı organ ağırlıkları ile yumurta ve albümün ağırlığı hariç yumurta kalite parametrelerini etkilememiştir. Serum glikoz, trigliserit, ürik asit ve AST düzeyleri SE-50 grubunda düşmüştür. 100 ppm SE ilavesi karaciğer ve kalp dokularında MDA düzeylerini düşürürken, karaciğer GSH-Px enzim aktivitesi her iki SE grubunda artmıştır. 100 ppm SE ilavesi göğüs etinde oleik asit ve MUFA düzeylerini düşürürken, linoleik, linolenik ve araşidonik asit, PUFA, toplam omega 6 ile omega 6/omega 3 düzeylerini önemli ölçüde yükseltmiştir. Sonuç olarak; soğuğa maruz kalan yumurtacı bıldırcı...

show abstract

“…GPx activity was measured by the method of Lawrence and Burk (14). One milliliter 50 nM PBS, pH 7.4, containing 5 mM EDTA, 2 µM NADPH, 20 µM GSH, 10 µM NaN 3 , and 23 mU glutathione reductase was incubated at 37°C for 5 min.…”

Section: Measurement Of Gpx Activitymentioning

confidence: 99%

Liver lipid peroxidation and antioxidant capacity in cerulein-induced acute pancreatitis

Batçıoğlu

Gül

Uyumlu

et al. 2009

Braz J Med Biol Res

View full text Add to dashboard Cite

The aim of this study was to evaluate the role of oxidative damage in pancreatitis-induced hepatic injury. Thirty-five rats were divided into five groups (each of 7 rats): control, cerulein (100 µg/kg body weight), cerulein and pentoxifylline (12 mg/kg body weight), cerulein plus L-NAME (10 mg/kg body weight) and cerulein plus L-arginine (160 mg/kg body weight). The degree of hepatic cell degeneration differed significantly between groups. Mean malondialdehyde levels were 7.00 ± 2.29, 20.89 ± 10.13, 11.52 ± 4.60, 18.69 ± 8.56, and 8.58 ± 3.68 nmol/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Mean catalase activity was 3.20 ± 0.83, 1.09 ± 0.35, 2.05 ± 0.91, 1.70 ± 0.60, and 2.85 ± 0.47 U/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively, and mean glutathione peroxidase activity was 0.72 ± 0.25, 0.33 ± 0.09, 0.37 ± 0.04, 0.34 ± 0.07 and 0.42 ± 0.1 U/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Cerulein-induced liver damage was accompanied by a significant increase in tis sue malondialdehyde levels (P < 0.05) and a significant decrease in catalase (P < 0.05) and GPx activities (P < 0.05). L-arginine and pentoxifylline, but not L-NAME, protected against this damage. Oxidative injury plays an important role not only in the pathogenesis of AP but also in pancre atitis-induced hepatic damage.

show abstract

Glutathione peroxidase activity in selenium-deficient rat liver

Cited by 3,137 publications

References 12 publications

Effect ofOcimum sanctum (Tulsi) and vitamin E on biochemical parameters and retinopathy in streptozotocin induced diabetic rats

Effect ofOcimum sanctum (Tulsi) and vitamin E on biochemical parameters and retinopathy in streptozotocin induced diabetic rats

Soğuk Stresine Maruz Bırakılan Japon Bıldırcınlarında Diyete İlave Edilen Çöven Ekstraktının Yumurtlama Performansı, Kan Biyokimyasal Parametreleri, Göğüs Eti Yağ Asidi Profili İle Karaciğer ve Kalp Dokusu Antioksidatif Potansiyeli Üzerine Etkisi

Liver lipid peroxidation and antioxidant capacity in cerulein-induced acute pancreatitis

Contact Info

Product

Resources

About