Liver lipid peroxidation and antioxidant capacity in cerulein-induced acute pancreatitis

Abstract

The aim of this study was to evaluate the role of oxidative damage in pancreatitis-induced hepatic injury. Thirty-five rats were divided into five groups (each of 7 rats): control, cerulein (100 µg/kg body weight), cerulein and pentoxifylline (12 mg/kg body weight), cerulein plus L-NAME (10 mg/kg body weight) and cerulein plus L-arginine (160 mg/kg body weight). The degree of hepatic cell degeneration differed significantly between groups. Mean malondialdehyde levels were 7.00 ± 2.29, 20.89 ± 10.13, 11.52 ± 4.60, 18.69 ± 8.56, and 8.58 ± 3.68 nmol/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Mean catalase activity was 3.20 ± 0.83, 1.09 ± 0.35, 2.05 ± 0.91, 1.70 ± 0.60, and 2.85 ± 0.47 U/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively, and mean glutathione peroxidase activity was 0.72 ± 0.25, 0.33 ± 0.09, 0.37 ± 0.04, 0.34 ± 0.07 and 0.42 ± 0.1 U/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Cerulein-induced liver damage was accompanied by a significant increase in tis sue malondialdehyde levels (P < 0.05) and a significant decrease in catalase (P < 0.05) and GPx activities (P < 0.05). L-arginine and pentoxifylline, but not L-NAME, protected against this damage. Oxidative injury plays an important role not only in the pathogenesis of AP but also in pancre atitis-induced hepatic damage.

Promoting effect of adipocytokine, apelin, on hepatic injury in caerulein-induced acute pancreatitis in rats

“…Free radicals produced by active leukocytes in liver may oxidize the active sites of the antioxidant enzymes, CAT and GSH, causing reduced activity, leading to hepatic cell lytic necrosis, when ROS production exceeds the antioxidant capacity, aggravating the condition. 43 Apelin markedly reduced (MDA) level, an indicator of free radical generation, improved the antioxidant enzymes, CAT and GSH, in AP group, trying to restore the oxidant/antioxidant balance, with improved hepatic function. The antioxidant effect of apelin observed in our study could be explained at least partially through its anti-inflammatory and inhibitory effect on hepatic leukocyte infiltration.…”

Medicinal Potential of Garcinia Species and Their Compounds

“…Garcinia cambogia increases lipidic peroxidation and positively regulates the expression of superoxide dismutase and glutathione peroxidase messenger ribonucleic acid (RNA) [ 130 ]. Lipidic peroxidation also increases oxidative stresss and can increase the risk of acute pancreatitis in patients using the species [ 131 ]. G. cambogia can cause other severe adverse events, including hepatoxicity and secondary acute hepatic insufficiency [ 124 , 132 ].…”

Therapeutic Effect of L-Carnitine on Acute Pancreatitis Induced by L-Arginine in Rats: Possible Role of Beclin Gene and Inducible Nitric Oxide Synthase

“…ACUTE Pancreatitis (AP) is a disease that varies and ranges from a mild and localized self-limiting disease to widespread and fatal multi-organ failure that may lead to death. Mild pancreatitis includes inflammation and edema but with the addition of necrosis and secondary injury to organs other than pancreas is considered to be severe pancreatitis [1,2] . No pharmacologic therapy is currently available to limit the disease and its progression inspite of its increased incidence [3] .…”