Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures

Borys, Jan; Maciejczyk, Mateusz; Antonowicz, Bożena; Krętowski, Adam; Sidun, Jarosław; Domel, Emilia; Dąbrowski, Jan; Ładny, Jerzy Robert; Morawska, Katarzyna

doi:10.3390/jcm8010127

Cited by 41 publications

(44 citation statements)

References 44 publications

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“…The contents of dityrosine, kynurenine, N-formylkynurenine and tryptophan were measured based on the characteristic fluorescence at 330/415, 365/480, 325/434 and 95/340 nm, respectively [ 38 ]. The results were normalized to the fluorescence of 0.1 mg/mL quinine sulphate in 0.1 M H 2 SO 4 [ 39 ].…”

Section: Methodsmentioning

confidence: 99%

High-Sugar Diet Disrupts Hypothalamic but Not Cerebral Cortex Redox Homeostasis

et al. 2020

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Despite several reports on the relationship between metabolic and neurodegenerative diseases, the effect of a high-sugar diet (HSD) on brain function is still unknown. Given the crucial role of oxidative stress in the pathogenesis of these disorders, this study was the first to compare the effect of an HSD on the activity of prooxidative enzymes, enzymatic and non-enzymatic antioxidants, and protein oxidative damage in the brain structures regulating energy metabolism (hypothalamus) and cognitive functions (cerebral cortex). Male Wistar rats were randomly divided into two groups (n = 10)—control diet (CD) and high-sugar diet (HSD)—for 8 weeks. We showed a decrease in glutathione peroxidase and superoxide dismutase activity and an increase in catalase activity in the hypothalamus of HSD rats compared to controls. The activity of xanthine oxidase and NADPH oxidase and the contents of oxidation (protein carbonyls), glycoxidation (dityrosine, kynurenine and N-formylkynurenine) and protein glycation products (advanced glycation end products and Amadori products) were significantly higher only in the hypothalamus of the study group. The HSD was also responsible for the disruption of antioxidant systems and oxidative damage to blood proteins, but we did not show any correlation between systemic redox homeostasis and the brain levels. In summary, HSD is responsible for disorders of enzymatic antioxidant defenses only at the central (plasma/serum) and hypothalamic levels but does not affect the cerebral cortex. The hypothalamus is much more sensitive to oxidative damage caused by an HSD than the cerebral cortex.

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Section: Methodsmentioning

confidence: 99%

High-Sugar Diet Disrupts Hypothalamic but Not Cerebral Cortex Redox Homeostasis

et al. 2020

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“…The available evidence indicates that thiol groups of glutathione are in balance with thiol groups of proteins, which enables the maintenance of functional activity of proteins as well as a reduced status of protein thiol groups [47]. With 40% decrease in glutathione concentration in NWS and 16.7% in SWS, a positive correlation between glutathione concentration and salivary amylase activity in both salivary glands is therefore not surprising and neither is 58% decrease in activity of this enzyme in NWS and 36.7% in SWS.…”

Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

Salivary Antioxidants and Oxidative Stress in Psoriatic Patients: Can Salivary Total Oxidant Status and Oxidative Status Index Be a Plaque Psoriasis Biomarker?

Skutnik-Radziszewska

Maciejczyk

Fejfer

et al. 2020

Oxidative Medicine and Cellular Longevity

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The aim of our research was to evaluate redox balance parameters and biomarkers of oxidative stress (OS) in nonstimulated and stimulated saliva as well as the blood of patients with plaque psoriasis compared to healthy controls. The study involved 40 patients with plaque psoriasis and 40 generally healthy subjects matched by age and gender to the study group patients. We assayed the concentration/activity of antioxidant enzymes: salivary peroxidase (Px), catalase (CAT), and superoxide dismutase (SOD) measured in unstimulated saliva (NWS), stimulated saliva (SWS), and erythrocytes. In plasma as well as NWS and SWS, we measured the concentration/activity of reduced glutathione (GSH), total antioxidant potential (TAC), total oxidative status (TOS), oxidative stress index (OSI), and markers of oxidative modification of proteins: advanced glycation end products (AGE), advanced oxidation protein products (AOPP), and lipid oxidation products: malondialdehyde (MDA) and total lipid hydroperoxide (LOOH). In NWS and SWS, we also evaluated the rate of reactive oxygen species (ROS) production. The concentration of Px, CAT, and SOD was significantly higher in NWS of patients with plaque psoriasis vs. healthy subjects. In SWS of psoriatic patients, we observed considerably higher concentration of Px and CAT, and in erythrocytes of patients with plaque psoriasis, the concentration of GPx and CAT was significantly higher compared to that in the controls. The levels of AOPP, AGE, MDA, and LOOH were considerably higher in NWS, SWS, and plasma of the study group compared to the controls. The concentration of total protein and salivary amylase was significantly lower in NWS and SWS of psoriatic patients compared to the healthy control. In the course of plaque psoriasis, we observed redox imbalances with prevalence of oxidation reactions. Mechanisms involved in the synthesis/secretion of proteins and activity of amylase were depressed in both glands of psoriatic patients; however, they were more inhibited in the parotid gland compared to the submandibular gland. TOS concentration and OSI value in NWS and SWS may serve as diagnostic biomarkers of plaque psoriasis.

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“…Nitric oxide (NO) concentration was measured colorimetrically using sulfanilamide and NEDA•2 HCl (N-(1-naphthyl)-ethylenediamine dihydrochloride). Nitrate was converted to nitrite using nitrate reductase and total NO was measured [40,41]. The absorbance was measured at 490 nm and NO concentration was expressed in µmol/mg protein.…”

Section: Nitrosative Stress Productsmentioning

confidence: 99%

Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney Disease

Maciejczyk

Szulimowska

Taranta-Janusz

et al. 2020

JCM

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This study is the first to evaluate protein glycooxidation products, lipid oxidative damage and nitrosative stress in non-stimulated (NWS) and stimulated whole saliva (SWS) of children with chronic kidney disease (CKD) divided into two subgroups: normal salivary secretion (n = 18) and hyposalivation (NWS flow < 0.2 mL min−1; n = 12). Hyposalivation was observed in all patients with severe renal failure (4–5 stage CKD), while saliva secretion > 0.2 mL/min in children with mild-moderate CKD (1–3 stage) and controls. Salivary amylase activity and total protein content were significantly lower in CKD children with hyposalivation compared to CKD patients with normal saliva secretion and control group. The fluorescence of protein glycooxidation products (kynurenine, N-formylkynurenine, advanced glycation end products), the content of oxidative damage to lipids (4-hydroxynonneal, 8-isoprostanes) and nitrosative stress (peroxynitrite, nitrotyrosine) were significantly higher in NWS, SWS, and plasma of CKD children with hyposalivation compared to patients with normal salivary secretion and healthy controls. In CKD group, salivary oxidation products correlated negatively with salivary flow rate, α-amylase activity and total protein content; however, salivary oxidation products do not reflect their plasma level. In conclusion, children with CKD suffer from salivary gland dysfunction. Oxidation of salivary proteins and lipids increases with CKD progression and deterioration of salivary gland function.

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Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures

Cited by 41 publications

References 44 publications

High-Sugar Diet Disrupts Hypothalamic but Not Cerebral Cortex Redox Homeostasis

High-Sugar Diet Disrupts Hypothalamic but Not Cerebral Cortex Redox Homeostasis

Salivary Antioxidants and Oxidative Stress in Psoriatic Patients: Can Salivary Total Oxidant Status and Oxidative Status Index Be a Plaque Psoriasis Biomarker?

Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney Disease

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