glutathione metabolism in rats fed high fat diet and effect of curcumin treatment

Abstract

The present research was designed to study the positive effect of curcumin and the bad effects of dietary high fat diet (beef tallow ) which causes( non alcoholic fatty liver disease), very harmful affecting health , also our study was designed to study the important role of curcumin, against( HFD-induced obesity) .Sixty male albino rats weighing( 165-170gm), were be obtained from animal house at the Faculty of Veterinary Medicine ,Zagazig University,Egypt .After acclimatization at 23-25 0Cand and free availability of water and diet rats were divided into four main groups,each contains 15 rats for 12 weeks.G1(Control),G2(Curcumin),G3(HFD),G4(HFD+cur) .At the end of the experiment , all rats were sacrified and blood collected weekly to biochemichal examination during 12 weeks. The main objective of this experiment was to determine the effects of curcumin supplementation on fat deposition and oxidative stress in the liver of rats with high fat (HF)diet-induced nonalcoholic fatty liver disease. Our results after statistical analysis revealed that showed that rats Fed on curcumin( 3%w/w), had a significant increase in (total glutathione ,GSH,glutathione quotient ) and a significant decrease in oxidized glutathione level in hepatic tissue ,a significant increase in the activity of liver glutathione peroxidase and reductase but a significant decrease in the glutathione state in rats fed on beef tallow on G3 (HFD) when compared to the control group .a significant increase in glutathione state in G4(HFD+CUR) when compared to G3(HFD). According to biochemical examinations, our results revealed a significant increase in insulin level ,HDL,and a significant decrease in blood glucose,TAG,total cholesterol,MDA ,LDL in G2(curcumin) and (HFD+CUR) but a small significant decrease in total bilirubin in only (HFD+CUR) group when compared to group 3(HFD) .A significant decrease in insulin level, HDL-c in G3(HFD)when compared to curcumin group ,a significant increase in blood glucose, TAG , TC ,LDL-c ,VLDL,MDA,bilirubin in high fat diet .Rats fed on curcumin 3% showed a significant decrease in body weight gain but rats fed on beef tallow revealed a significant increase in body weight .