Glutathione and related enzyme activities in the blood of Simmental bulls

Križanović, Dubravka; Božić, Pero; Vrbanac, Bojana Gradinski; Karadjole, Ivo; Petruša, V.P

doi:10.1016/s0034-5288(97)90017-5

Research in Veterinary Science

1997

DOI: 10.1016/s0034-5288(97)90017-5

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Glutathione and related enzyme activities in the blood of Simmental bulls

Dubravka Križanović

Pero Božić²,

Bojana Gradinski Vrbanac³

et al.

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“…Reduced glutathione fulfills several essential functions such as detoxification of oxygen-derived free radicals and storage and transfer of cysteine [12]. Cellular glutathione and related enzymes such as glutathione peroxidase, glutathione S-transferase and glutathione reductase are among the principal protective mechanisms against endogenous and exogenous toxic substances and free radicals-mediated damage in liver tissue as well as in other tissues [5,8,9,13,14].…”

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Total Glutathione and Glutathione Reductase in Bovine Erythrocytes and Liver Biopsy

Ellah

Okada

Goryo

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. The goal of the present study was to measure the total glutathione level and glutathione reductase activity in bovine erythrocytes and liver biopsy. Five cows were injected intraperitoneally with DL-ethionine (12.5 mg/kg B.W.), and two control cows were injected with normal saline (0.9% NaCl). Ultrasonography guided liver biopsy, and blood samples were collected at 0, 4, 7 and 10 days after injection. The hepatic total glutathione level was significantly increased on Days 7 (p<0.05) and 10 (p<0.01), and hepatic glutathione reductase activity was significantly increased on Days 4 (p<0.05), 7 (p<0.01) and 10 (p<0.01). There were insignificant changes in the erythrocytic total glutathione level. The present study demonstrated that liver biopsy is a valuable tool for detecting oxidative stress and for diagnosing hepatic dysfunction in cattle from the viewpoint of the status of glutathione and glutathione reductase. KEY WORDS: bovine liver biopsy, glutathione, glutathione reductase.

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mentioning

confidence: 99%

Total Glutathione and Glutathione Reductase in Bovine Erythrocytes and Liver Biopsy

Ellah

Okada

Goryo

et al. 2008

The Journal of Veterinary Medical Science

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Serum Adenosine Deaminase Activity in Bovine Liver Diseases

Ellah

Nishimori

Goryo

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. A total of 60 cattle were examined for the presence of pathological liver lesions. The liver lesions were classified as glycoge n degeneration, liver abscess, sawdust liver and fatty degeneration. The value of serum adenosine deaminase (ADA) activity was investigated as a pilot study for diagnosing liver diseases in cattle. Serum ADA activity was significantly higher in cases with glycogen degeneration (9.8 ± 3.8 U/l) , liver abscess(10.4 ± 3.2 U/l) , sawdust liver (11.5 ± 7.3U/l) and fatty degeneration (20.8 ± 7.7 U/l) than in the controls. The results indicate that ADA activity increases with the degree of hepatocellular damage. We concluded that serum ADA activity may be of value in bovine liver disease diagnosis. KEY WORDS: adenosine deaminase, bovine, liver disease.J. Vet. Med. Sci. 66(11): 1421-1422, 2004 Adenosine deaminase (ADA) is a purine catabolic enzyme ubiquitous in mammalian tissue with the highest concentration in lymphoid tissues. In vitro it catalyzes deamination of both adenosine and 2'-deoxyadenosine to inosine and 2'-deoxyinosine, respectively [3,15]. It was conjectured that its primary function is the detoxification of pharmacologically active adenosine [19]. ADA activity was significantly elevated in sera from human patients with hepatic diseases, hematological malignancies and infectious diseases [12,15]. It is the most useful single test in portal cirrhosis [12]. In bovine studies, it has been reported that elevation of serum ADA activity is accompanied by increase of mononuclear cell infiltration in the liver [9,22] and has been observed in cases of clinical bovine leukemia [23]. We investigated the value of serum adenosine deaminase (ADA) activity as a pilot study for diagnosing liver diseases in cattle, together with the histopathological examination of the affected livers.A total of 60 cattle slaughtered at Asahikawa slaughterhouse (Asahikawa, Hokkaido, Japan), or examined by autopsy at Morioka Livestock Hygiene Center (Morioka, Iwate, Japan), were studied. After collection of blood samples, each animal was examined for presence of pathological lesions. The collected liver samples were kept in (10%) neutral buffered formalin and examined histopathologically [2]. Animals with no abnormal clinical signs and no gross post mortem pathological changes in the liver were used as controls. Types of liver diseases were classified according to the gross and histopathological findings, which were diagnosed as glycogen degeneration, liver abscess, sawdust liver and fatty liver. Histopathological examination of the livers showed the presence of irregular clear spaces in the cytoplasm of the hepatocytes in glycogen degeneration, focal areas of necrosis with neutrophilic infiltration in sawdust liver and rounded fat globules in the hepatocytes that displaced their contents to one side as in severe fatty degeneration.The serum samples were used to measure serum activities of ADA, aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH) and γ-glutamyl transferase (γ-GT). The activiti...

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Glutathione and related enzyme activities in the blood of Simmental bulls

Cited by 2 publications

References 17 publications

Total Glutathione and Glutathione Reductase in Bovine Erythrocytes and Liver Biopsy

Total Glutathione and Glutathione Reductase in Bovine Erythrocytes and Liver Biopsy

Serum Adenosine Deaminase Activity in Bovine Liver Diseases

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