Glutathione and glutathione-metabolizing enzymes in the erythrocytes of healthy children and in children with insulin-dependent diabetes mellitus, juvenile rheumatoid arthritis, coeliac disease and acute lymphoblastic leukaemia

Mr, Ståhlberg; Hietanen, Eero

doi:10.3109/00365519109091097

Cited by 15 publications

(11 citation statements)

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“…On the other hand, GPx activity positively correlated with the GSH concentration. Decreased activities of GSHrelated enzymes in enterocytes from intestinal biopsies with villous atrophy, as well as in erythrocytes of paediatric patients with coeliac disease 26,27 have been reported. In vitro studies by Dol¢ni et al 28 showed that gliadin peptides provoked a signi¢cant decrease in cell GSH content and the activities of GSH-related enzymes.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant enzymes, glutathione and lipid peroxidation in peripheral blood of children affected by coeliac disease

Stojiljković

Todorović²,

Radlovic

et al. 2007

Ann Clin Biochem

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Section: Discussionmentioning

confidence: 99%

Antioxidant enzymes, glutathione and lipid peroxidation in peripheral blood of children affected by coeliac disease

Stojiljković

Todorović²,

Radlovic

et al. 2007

Ann Clin Biochem

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“…The GSH depletion is followed by a decrease of the GPx and GR activities. A reduced GPx activity has been found in biopsies and erythrocytes taken from celiac patients with severe villous atrophy (Ståhlberg et al 1988, Ståhlberg andHietanen 1991). Several in vitro studies have reported pro-oxidative effects of gliadin in human intestinal cell cultures (Dolfini 2002, Dolfini 2005.…”

Section: Discussionmentioning

confidence: 99%

Glutathione redox cycle in small intestinal mucosa and peripheral blood of pediatric celiac disease patients

Stojiljković

Pejić

Kasapović

et al. 2012

An. Acad. Bras. Ciênc.

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The celiac disease is an autoimmune gastrointestinal disorder caused by gluten from wheat, rye or barley. In genetically predisposed persons, gluten induces the immune-mediated inflammation of small intestinal mucosa. Histological lesions include intraepithelial lymphocytosis, crypt hypertrophy and villous atrophy, resulting in malabsorption of micro-and macronutrients. The only treatment for celiac patients is a permanent gluten-free diet (GFD). Reactive oxygen species (ROS) and oxidative stress are strongly associated with the celiac disease. Glutathione (GSH) is a main detoxifier of endogenous and exogenous ROS in the intestine. In order to explain the role of glutathione redox cycle in celiac patients, we examined the activities of GSHrelated antioxidant (AO) enzymes glutathione peroxidase (GPx) and glutathione reductase (GR), as well as the concentration of GSH in small intestinal biopsies and peripheral blood of children affected by the celiac disease. The concentration of lipid hydroperoxides (LOOH) as markers of oxidative damage was measured in the same samples. The results clearly demonstrate a significant malfunction of GSH redox cycle with a concomitant decrease in the capacity to regenerate GSH and detoxify LOOH in celiac patients, even after several years of GFD. The oral administration of GSH and a diet rich in natural antioxidants, as well as appropriate dietary supplements, could be of great benefit to the patients.

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“…Many investigators have reported lower concentrations of glutathione in the erythrocytes, aorta, and lenses of diabetic patients compared with healthy subjects (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19), except for some studies that reported similar glutathione concentrations (20). In addition, an increase in malondialdehyde concentration has been reported in erythrocytes and other tissues of diabetic animals and patients (21)(22)(23)(24)(25)(26)(27)(28).…”

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confidence: 99%

“…In addition to oxidant-antioxidant balance, the decreased level of glutathione in diabetic patients could be influenced by the decreased activity of enzymes such as ␥-glutamylcysteine synthetase and glutathione reductase, possibly because of their glycation by hyperglycemia (12)(13)(14). Previous studies have reported that vitamin E in vitro (54) and vitamin E supplementation to diabetic patients can lower glycosylation of proteins (45)(46)(47).…”

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confidence: 99%

Vitamin E supplementation restores glutathione and malondialdehyde to normal concentrations in erythrocytes of type 1 diabetic children.

2000

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OBJECTIVE -This study examined the relationship between cellular glutathione and vitamin E concentrations and the effect of vitamin E (␣-tocopherol) supplementation on glutathione and lipid peroxidation product concentrations in the erythrocytes of type 1 diabetic patients.RESEARCH DESIGN AND METHODS -We obtained written informed consent to participate in this study from diabetic patients (n = 29) and their age-matched nondiabetic siblings (n = 21) according to the guidelines of the Institutional Review Board on Human Experimentation. Diabetic patients were supplemented with a DL-␣-tocopherol (vitamin E) capsule (100 IU/ orally) or placebo for 3 months in a double-blind clinical trial. Fasting blood samples were collected from each diabetic patient before the start of and after the 3 months of vitamin E or placebo supplementation. Glutathione, malondialdehyde (which is a product of lipid peroxidation), and ␣-tocopherol were determined using high-performance liquid chromatography. A total of 5 diabetic patients were excluded after randomization from the data analyses. Data were analyzed statistically using a paired Student' s t test to compare 12 diabetic patients taking vitamin E with 12 diabetic patients receiving placebo supplementation and to compare diabetic patients with healthy nondiabetic subjects.RESULTS -Erythrocytes of diabetic patients had 21% higher (P Ͻ 0.001) malondialdehyde and 15% lower (P Ͻ 0.05) glutathione concentrations than healthy subjects. Vitamin E in erythrocytes had a significant correlation with the glutathione concentrations in the erythrocytes (r = 0.46, P Ͻ 0.02). Vitamin E supplementation increased glutathione concentrations by 9% (P Ͻ 0.01) and lowered concentrations of malondialdehyde by 23% (P Ͻ 0.001) and of HbA 1c by 16% (P Ͻ 0.02) in erythrocytes of diabetic patients. No differences were evident in these parameters before versus after placebo supplementation.CONCLUSIONS -Glutathione level is significantly related to vitamin E level, and supplementation with vitamin E (100 IU/day) significantly increases glutathione and lowers lipid peroxidation and HbA 1c concentrations in the erythrocytes of type 1 diabetic patients.

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Glutathione and glutathione-metabolizing enzymes in the erythrocytes of healthy children and in children with insulin-dependent diabetes mellitus, juvenile rheumatoid arthritis, coeliac disease and acute lymphoblastic leukaemia

Cited by 15 publications

References 0 publications

Antioxidant enzymes, glutathione and lipid peroxidation in peripheral blood of children affected by coeliac disease

Antioxidant enzymes, glutathione and lipid peroxidation in peripheral blood of children affected by coeliac disease

Glutathione redox cycle in small intestinal mucosa and peripheral blood of pediatric celiac disease patients

Vitamin E supplementation restores glutathione and malondialdehyde to normal concentrations in erythrocytes of type 1 diabetic children.

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