Glutaraldehyde Cross-linking Modification of Decellularized Rat Kidney Scaffolds

Abstract

The decellularized (DC) scaffolds retain three-dimensional structures for the stimulation of cell growth, with components of the extracellular matrix (ECM) relatively conserved between species. The strategy based on decellularized scaffolds provides a new way for organ regeneration, with a number of prominent advances having been reported in the past few years. While their lack of biomechanical strength and excessive degradation limit the clinical applications, therefore it is urgent to modify the DC scaffolds to improve the performance.In this article we describe a simple and robust modification protocol for DC rat kidney scaffolds. To modify, we perfuse DC rat kidneys with glutaraldehyde through the perfusion circulation of the decellularization. After cross-linking, kidney scaffolds are harvested for evaluation of histology, structural stability, and biocompatibility, involving water absorption testing, biomechanical testing, scanning electron microscopy, and several different histological and immunofluorescent analyses.

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

“…Since decellularization often has adverse effects on the biomechanical properties of ECM, physical and chemical crosslinking methods are usually used to preserve the 3D structure of dECM and improve the mechanical properties of scaffolds [ 166 ]. Glutaraldehyde is a commonly used chemical crosslinker for collagen-based materials, and Yu cross-linked decellularized kidney scaffold with glutaraldehyde perfusion; but its cytotoxicity may affect subsequent studies [ 167 , 168 ]. Alternatively, glyoxal and genipin were used as crosslinkers with reduced cytotoxicity [ 169 , 170 ].…”

Liver tissue-derived ECM loaded nanocellulose-alginate-TCP composite beads for accelerated bone regeneration

“…Since biodegradation of β-TCP was very negligible without the action of osteo cells (specially osteoclast and multi-nucleus cells) at physiologic pH [39], TAC beads showed the lowest weight loss behavior (figure 3(B)). However, ECM proteins are very susceptible to degradation [41]. This might be why β-TCP beads containing ECM protein (ETAC) showed moderate weight loss properties.…”

Biomedical Application of Decellularized Scaffolds

“…148 Yu et al perfused rat kidney with glutaraldehyde through an decellularized perfusion cycle. 149 The kidney scaffold obtained after cross-linking not only retains the original unique characteristics but also has good mechanical properties.…”

“…Wang et al reduced the immunogenicity of decellularized whole pig liver matrix by cross-linking with natural new agents glutaraldehyde or genipin . Yu et al perfused rat kidney with glutaraldehyde through an decellularized perfusion cycle . The kidney scaffold obtained after cross-linking not only retains the original unique characteristics but also has good mechanical properties.…”