?-Glutamyltranspeptidase-Aktivit�t im Urin bei urorenalen Krankheiten

Levy, A; Uc, Dubach

doi:10.1007/bf01497571

Cited by 19 publications

(6 citation statements)

References 11 publications

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“…(12) also reported sex differences for the excretion of alanine aminopeptidase, males showing higher alanine aminopeptidase-activities than females. On the other hand, Szasz (13) found sex-dependent differences in the activity of -glutamyltransferase, whereas Levy & Dubach (14) could not reproduce these data. The different ages of volunteers is one possible source of these discrepancies.…”

Section: Discussionmentioning

confidence: 90%

Enzymuria of the Rat: Biorhythms and Sex Differences

Grötsch¹,

Hropot²,

Klaus³

et al. 1985

Clinical Chemistry and Laboratory Medicine

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Grötsch, Hropot, Klaus, Malerczyk and Mattenheimer: Enzymuria of the rat: Biorhythms and sex differences 343 Summary: Alanine aminopeptidase, -glutamyltransferase and N-acetyl-ß-Z)-glucosaminidase were measured daily over 65 days in 24-hour urine of male and female Wistar rats. The mathematical evaluation was based on the Föw/er-analysis. The excretion of alanine aminopeptidase and -glutamyltransferase was higher in male than in female rats. This sex-dependent difference was not observed for N-acetyl-ß-/)-glucosaminidase. The excretion of the 3 enzymes followed a biorhythm with a dominant period of 7 days forglutamyltransferase and N-acetyl-ß-£>-glucosaminidase and one of 9 days for alanine aminopeptidase. Biorhythms and sex differences of enzymuria should be considered in experimental designs. Enzymurie der Ratte: Biorhythmen und GeschlechtsdifferenzenZusammenfassung: Alaninaminopeptidase, -Glutamyltransferase und N-Acetyl-ß-D-glucosaminidase wurden täglich über 65 Tage bei männlichen und weiblichen Wistar-Ratten im 24 h-Urin gemessen. Die Ergebnisse wurden vermittels fbwr/er-Anälyse ausgewertet. Die Ausscheidung von Alaninaminopeptidase und -Glutamyltransferase zeigte deutliche geschlechtsabhängige Unterschiede, wobei von männlichen Ratten mehr ausgeschieden wurde als von weiblichen Tieren. N-Acetyl-ß-/)-glucosaminidase zeigt diese Unterschiede nicht. Die Ausscheidung der drei Enzyme unterlag Biorhythmen mit Periodenlängen von sieben Tagen für -Glutamyltransferase und N-Acetyl-ß-jp-glucosaminidase und neun Tagen für Alaninaminopeptidase. Biorhythmen und Geschlecht$differenzen in der Enzymurie sind bei Versuchsplanungen zu berücksichtigen. Introduction. , « , , years the rat has been used äs the main experimental Urine enzymes äre being used with increasing animal in enzyine excretion studies. This animal frequency äs sensitive indicatöfs for early detection shows significant enzymuria when substances are of renal damage. Many investigatiöns include a administered which cause increased enzymuria in discussipn of biörhythms in man and animals, re-man. The rat tolerates well single-cage housing in speetively (l -3), A few reports on experimental ani-long term experiments. Enzymes with the smallest mal models document this coherence of biörhythms experimental Variation and good reproducibility are and nephrptoxicity with a close correlation between alanine aminopeptidase, -glutamyltransferase and enzyme excretion and renal damage. Of the large N-acetyl-ß^jD-glucosaminidase. The aim of this study nuinber of urine enzymes, it is mainly those from the was to apply modern mathematical methods to the proximal tübular cells that are of particular interest investigation of biörhythms and sex-specific for toxicological and clinical investigatiöns. In recent differences in the excretion of these enzymes over a

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Section: Discussionmentioning

confidence: 90%

Enzymuria of the Rat: Biorhythms and Sex Differences

Grötsch¹,

Hropot²,

Klaus³

et al. 1985

Clinical Chemistry and Laboratory Medicine

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“…N-Acetyl-ß-Z>-glucosaminidase, alkalischer Phosphatase und Alaninaminopeptidase in der Referenzgruppe gut mit den in der Literatur angegebenen Werten für Normalpersonen überein (10,(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)…”

Section: Resultateunclassified

Bestimmung von Enzymaktivitäten in Urinen von Calciumoxalatstein-Patienten

Prinsen¹,

Günther²,

Breuer³

1986

Clinical Chemistry and Laboratory Medicine

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Zusammenfassung: Die katalytischen Aktivitäten von Lactatdehydrogenase, -Glutamyltransferase, alkalischer Phosphatase, Alaninaminopeptidase und N-Acetyl-ß-/)-glucosaminidase wurden im 24-Stunden-Urin von Calciumoxalatstein-Patienten (9 Männer, 11 Frauen) gemessen und mit denen von einem Normalkollektiv (11 Männer, 10 Frauen) verglichen. Die diagnostische Sensitivität und die diagnostische Spezifität wurden nach vier verschiedenen Diskriminationsverfahren berechnet, wobei die Sensitivität zwischen 65%-l 00% lag, die Spezifität zwischen 72%-l00%.Beim Normalkollektiv war eine deutliche Korrelation in der Ausscheidung der drei Bürstensaumenzyme vorhanden, während bei den Calciumoxalatstein-Patienten nur eine Korrelation zwischen -Glutamyltransferase-und N-Acetyl-ß-/)-glucosaminidase-Exkretion nachzuweisen war. Hieraus lassen sich pathologische Schlüsse ziehen. Determination of catalytic activities in urines ofpatients with calcium urolithiasis Summary:The catalytic activity of lactate dehydrogenase, -glutamyltransferase, alkaline phosphatase, alanine aminopeptidäse and N-acetyl-ß-D-glucosaminidase has been measured in 24 h urines of patients with a calcium oxalate calculus (9 men, 11 women) and has been compared with those of a reference collective (11 men, 10 women). The diagnostic sensitivity and the diagnostic specificity have been calculated according to four different discrimination methods in which the diagnostic sensitivity lies between 65% and 100%, the diagnostic specificity between 72% and 100%.Within the reference group there was a correlation between the excretion of the three brush-border enzymes, whereas within the group of patients only a correlation between -glutamyltransferase and N-acetyl-ß-Dglucosanünidase was observed. From this pathophysiological conclusions can be drawn.

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“…The more, since it is almost totally absent in any other part of the kidney and of the urinary tract (1,2, 9). The available data concerning the excretion rate of yGT in hu mans were collected in acute cases and are somewhat contradictory (15,19,37). In the rat in the course of acute experiments excre tion of yGT proved to be a reliable indicator of even transient 02 or substrate deficiency (7).…”

mentioning

confidence: 99%

Enzymuria (the Output of Gamma-Glutamyl-Transpeptidase and of N-Acetyl-Beta-D-Glucosaminidase) in the Course of Experimental Renovascular Hypertension

Mályusz¹,

Braun²

1981

Enzyme

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The urinary output of γ-glutamyl-transpeptidase (γGT) and of N-acetyl-β-D-glucosaminidase (NAG) was studied in rats with ‘two-kidney Goldblatt hypertension’ 3-6, 16-19 and 30-33 weeks after eliciting high blood pressure. γGT excretion rate of normotensive males was higher than that of females, while the activity of the renal tissue was on the same level. γGT output of hypertensive males was elevated in the early and in the middle stages of the disease, it was subnormal in the late stage. In females γGT output increased in animals with excessively high blood pressure (> 200 mm Hg) only. γGT output correlated with the tissue activity in males only. In all animals there was an inverse, linear correlation between tissue γGT activity and the hydroxyproline content. The pattern of the NAG output was similar to that of γGT, however, excretion of NAG showed no sex differences and remained high in the late stage of the disease, too. Nephrosclerosis was less pronounced in female Goldblatt rats than in males.

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?-Glutamyltranspeptidase-Aktivit�t im Urin bei urorenalen Krankheiten

Cited by 19 publications

References 11 publications

Enzymuria of the Rat: Biorhythms and Sex Differences

Enzymuria of the Rat: Biorhythms and Sex Differences

Bestimmung von Enzymaktivitäten in Urinen von Calciumoxalatstein-Patienten

Enzymuria (the Output of Gamma-Glutamyl-Transpeptidase and of N-Acetyl-Beta-D-Glucosaminidase) in the Course of Experimental Renovascular Hypertension

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