Enzymuria (the Output of Gamma-Glutamyl-Transpeptidase
and of N-Acetyl-Beta-D-Glucosaminidase) in the Course of
Experimental Renovascular Hypertension

Mályusz, M.; Braun, Diana

doi:10.1159/000459144

Cited by 10 publications

(4 citation statements)

References 8 publications

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“…as described by us recently [17], On the other hand, in testosterone-substituted castrates even the highest specific renal yGT activity did not result in an enzymuria larger than that in uncastrated males with lower specific activity. This result is in conflict with the cited correlation.…”

Section: Discussionsupporting

confidence: 71%

“…Introduction of y-glutamyl transpeptidase (yGT) and of Nacetyl-ß-Z)-glucosaminidase (NAG), -i.e., both of membrane-bound and lysosomal en zymes -in the rat with experimental renovas cular hypertension [17], It could be demon strated that especially the early phases of the disease are easy to recognize due to the ele vated output of both enzymes. At the same time, however, we found great differences in the excretion rate of yGT between male and female rats.…”

mentioning

confidence: 99%

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Effect of Castration and Testosterone Substitution on the Urinary Output of Gamma-Glutamyl Transpeptidase and of N-Acetyl-Beta-D-GIucosaminidase of Male Rats with Renovascular Hypertension

Mályusz¹,

Ehrens²

1983

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The effect of castration of male rats with experimental renal hypertension (‘two kidney Goldblatt hypertension’) was studied on the height of the hypertension and on the urinary output of γ-glutamyl transpeptidase (γGT) and of N-acetyl-beta-D-glucosaminidase (NAG). Castration was carried out immediately after clamping one renal artery. Some of the castrates received testosterone substitution from the 3rd postoperative week onwards. Uncastrated hypertensive males served as controls. The experiments were carried out 8-18 weeks after eliciting high blood pressure. Hypertension as well as enzymuria were less expressed in castrates than in uncastrated males or in testosterone-substituted rats. In all animals studied the γGT excretion rate showed a positive correlation with the blood pressure. The output of γGT and of NAG as well as the specific γGT activity of the renal membrane fraction was lower in castrates than in uncastrated males or in substituted castrates. In uncastrated males and in testosterone-substituted castrates the daily NAG output showed a direct correlation with the renal hydroxyproline content. No such correlation was found in castrated males. The kidneys of castrates and of testosterone-substituted castrates contained less hydroxyproline than those of uncastrated males.

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Section: Discussionsupporting

confidence: 71%

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confidence: 99%

Effect of Castration and Testosterone Substitution on the Urinary Output of Gamma-Glutamyl Transpeptidase and of N-Acetyl-Beta-D-GIucosaminidase of Male Rats with Renovascular Hypertension

Mályusz¹,

Ehrens²

1983

Enzyme

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“…Similarly to our results, the predicting ability of KIM-1 reduced over time, with an AUC between 0.52 and 0.84 within 72 h from injury (Lianghos et al 2009). The relatively lower urine levels of KIM-1 in azotemic AKI dogs, compared with non-azotemic AKI dogs might also reflect a tubular enzyme depletion with progression of tubular damage and time, as previously reported in a murine model of AKI (Malyusz and Braun 1981). Different elevation in urine KIM-1/uCr in AKI dogs might also be influenced by the kind of tubular damage.…”

Section: Discussionsupporting

confidence: 60%

Clinical utility of urine kidney injury molecule-1 (KIM-1) and gamma-glutamyl transferase (GGT) in the diagnosis of canine acute kidney injury

et al. 2018

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The aim of the present study was to evaluate the sensitivity and specificity of urine KIM-1 and urine GGT for the detection of naturally-occurring AKI, compared to healthy control dogs, dogs with stable chronic kidney disease (CKD), and dogs with lower urinary tract disorders (LUTD). The study included AKI grade 1 (n = 21), AKI grade 2 to 5 (n = 11), stable CKD (n = 11), LUTD (n = 15), and healthy dogs (n = 37). Urine KIM-1 (ng/mg) and GGT (U/l) were normalized to urine creatinine (uCr). Statistically significant difference in KIM/uCr (p = 0.0007) and GGT/uCr (p < 0.0001) was found among the study groups. Area under the curve (AUC) for KIM-1/uCr and GGT/uCr as predictors of AKI was 0.81 and 0.91 respectively. Values of KIM-1/uCr of 0.73 ng/mg and of GGT/uCr of 54.33 showed the best combination of sensitivity and specificity (75% and 75.6%; 85.7% and 89.1% respectively). A significant positive correlation (p < 0.0001) between KIM-1/uCr and GGT/uCr was found. Both urine KIM-1/uCr and GGT/uCr seemed to be potentially good markers for the diagnosis of AKI. Dogs with AKI showed significantly higher levels of urine KIM-1/uCr and urine GGT/uCr, compared with healthy dogs. Caution should be used in the evaluation of elevated urine KIM-1/uCr and GGT/uCr in dogs with pre-existing CKD and/or LUTD. Urine KIM-1/uCr and GGT/uCr might have a significant clinical utility, as complementary test, particularly in diagnosis early, non-azotemic stages of AKI.

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“…Urine was collected daily. The collecting vessels contained 1 ml of 100 mmol/1 Tris-HCl buffer (pH 7.6 at 20 °C) to stabilize urinary pH [8].…”

Section: Methodsmentioning

confidence: 99%

Differences in the Renal Handling of Pancreatic and Salivary Amylase in the Rat

Mályusz¹,

Wrigge²,

Caliebe³

et al. 1990

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Plasma activity and excretion of pancreatic (P) amylase in the rat was found to be negligible. In contrast, the excretion rate of salivary (S) amylase was substantial and variable, depending on diuresis. P-amylase had a higher isoelectric point, a greater sieving coefficient, and a shorter half-life than S-amylase. A bolus injection of 125I-labelled enzymes was followed by the appearance of 125I-labelled enzyme- as well as protein-free 125I activity in the urine. The enzyme loss was smaller and the fraction of protein-free 125I activity higher following injection of P-amylase. The affinity of P-amylase to paraffin oil exceeded that of S-amylase in partition experiments with water and paraffin oil in vitro. It is concluded that both renal filtration and reabsorption of P-amylase exceed those of S-amylase. This might be due to the higher lipophility of P-amylase in comparison to the salivary type.

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Enzymuria (the Output of Gamma-Glutamyl-Transpeptidase and of N-Acetyl-Beta-D-Glucosaminidase) in the Course of Experimental Renovascular Hypertension

Cited by 10 publications

References 8 publications

Effect of Castration and Testosterone Substitution on the Urinary Output of Gamma-Glutamyl Transpeptidase and of N-Acetyl-Beta-D-GIucosaminidase of Male Rats with Renovascular Hypertension

Effect of Castration and Testosterone Substitution on the Urinary Output of Gamma-Glutamyl Transpeptidase and of N-Acetyl-Beta-D-GIucosaminidase of Male Rats with Renovascular Hypertension

Clinical utility of urine kidney injury molecule-1 (KIM-1) and gamma-glutamyl transferase (GGT) in the diagnosis of canine acute kidney injury

Differences in the Renal Handling of Pancreatic and Salivary Amylase in the Rat

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