Neonatal diet may influence the development of type 1 diabetes (T1D) in susceptible individuals through an intestinal mucosal inflammatory response, resulting in loss of self-tolerance. We tested the hypothesis that formula feeding during the neonatal period accelerates the development of T1D in diabetes-prone BioBreeding (BBDP) rats through regulation of CD4ϩCD25ϩ regulatory T lymphocytes (T reg ) and anti-inflammatory cytokines. BBDP rat pups fed rat milk substitute (RMS) via a "pup-in-the cup" system were compared with mother-fed (MF) rats. The spleen and thymus were analyzed for Foxp3-expressing CD4ϩ/CD25ϩ T cells. Multiplex enzyme-linked immunosorbent assays (ELISAs) were performed to measure cytokine-induced neutrophil chemoattractant (CINC), tumor necrosis factor ␣ (TNF-␣), interferon-gamma (IFN-␥), interleukin (IL)-4, IL-10, and IL-18. Diabetes-free survival, time of disease onset, and T reg /total T lymphocyte ratios were not different. MF pups had higher ileal CINC (p Ͻ 0.001) and IL-18 (p ϭ 0.002), but no differences in the liver. There were no differences in ileal cytokine concentrations of 75-d-old rats, but the formula-fed rats had greater liver TNF-␣ (p Ͻ 0. T ype 1 diabetes (T1D) is characterized by the autoimmune destruction of insulin-secreting beta cells, resulting in insulin deficiency and hyperglycemia in genetically susceptible individuals. While genetic predisposition is clearly a major component of T1D, interactions between the environment and the immune system are thought to weigh heavily in disease development (1). Indeed, with evidence pointing to the pathogenesis of T1D beginning very early in life or perhaps in utero (2), more attention has recently been directed toward environmental exposures early in infancy, particularly that of diet.An underlying structural or developmental defect in the gastrointestinal tract may assist in the entry of dietary agents that have the potential to influence the T1D process (3,4). In healthy individuals, the small intestine acts as a gatekeeper between the external environment and the internal organ systems of the body, allowing the absorption of nutrients and preventing the passage of potential antigens. During infancy, especially in the first 2 mo of life, increased intestinal permeability allows dietary antigens greater access to the resident immune cells of the lamina propria (5). Increased gut permeability has also been reported in T1D patients who do not have associated celiac disease (6).BioBreeding diabetes-prone (BBDP) rats (Biomedical Research Models, Worcester, MA) are a well-studied model of T1D because at a predictable point in their life span (70 -120 d), a large majority (Ͼ90%) develops T1D manifested as overt glycosuria, hyperglycemia, and ketosis (7). The loss of tolerance that permits autoimmunity against the beta cell and the development of T1D (8) is largely mediated by CD4ϩCD25ϩ regulatory T lymphocytes T reg , which suppress the activity of effector T cells carrying destructive capacity. A decrease in T reg spleen cell...