Glulathione-S-transferases Gene Polymorphism in Prediction of Gastric Cancer Risk by Smoking and Helicobacter Pylori Infection Status

Glutathione S-transferase (GST) enzyme levels are associated with risk of many cancers, including hematologic tumours. We here aimed to investigate the relationships between GSTM1, GSTT1 and GSTP1 polymorphisms and the risk of AML. Genotyping of GSTs was based upon duplex polymerase-chain-reactions with the confrontingtwo-pair primer (PCR-CTPP) method in 163 cases and 204 controls. Individuals carrying null GSTT1 genotype had a 1.64 fold risk of acute leukemia relative to a non-null genotype (P<0.05). A heavy risk was observed in those carrying combination of null genotypes of GSTM1 and GSTT1 and GSTP1 Val allele genotypes when compared with those carrying wild genotypes, with an OR (95% CI) of 3.39 (1.26-9.26) (P<0.05). These findings indicate that genetic variants of GST and especially the GSTT1 gene have a critical function in the development of AML. Our study offers important insights into the molecular etiology of AML.

Section: Discussionmentioning

confidence: 54%

Risk Effects of GST Gene Polymorphisms in Patients with Acute Myeloid Leukemia: A Prospective Study

Zhou

Zhu²,

Zhang³

et al. 2013

“…The pathogen has a strong association with a variety of gastric pathologies, including type B antral gastritis, peptic ulcer, gastric MALT lymphoma, and gastric adenocarcinoma (Jing et al, 2012). It is consider that H. pylori infection is the most common cause of morbidity and mortality in upper digestive tract diseases.…”

Section: Introductionmentioning

confidence: 99%

Factors Associated with Helicobacter Pylori Infection, Results from a Developing Country-Pakistan

Valliani

Khan²,

Chagani³

et al. 2013

Background: It is known that blood group antigens are related to the development of peptic ulcer and gastric carcinoma. Infections due to H. pylori are most widespread among the developing regions due to poor standard of public health. This study sought to determine the association of H. pylori with ABO blood groups, age, gender, and smoking status among inpatients at a public sector hospital in Karachi. Materials and Methods: A cross-sectional study was conducted at endoscopy suite at a public sector hospital in Karachi in the year 2011. All the symptomatic patients coming for upper GIT endoscopy were included in this study. Results: Biopsy for histopathology was taken from 93 patients, with an age range from 15-65 years. Age group of 15 to 20 years was found to be associated with H. pylori infection but without significance (p-value 0.83). In all, 36 (38.7%) turned out to be H. pylori positive with a significant male preponderance (p=0.04). Distribution of ABO blood groups in H. pylori positive group were A=31.4%, B=15.4%, AB=25.0% and O=53.7%, with a statistically significant link for blood group O (p=0.05) . Rhesus factor was also compared but significant relationship was evident (p-value 0.73). Conclusions: This study demonstrated that H. pylori infection can be related to ABO blood group, middle age persons and male gender. People of blood group O are more prone to develop infection related gastritis, ulcers, and even perforations, so they should be more cautious against transmission of the bacterium.

“…The GSTM1 null genotype was found to be high in stomach cancer cases (55.9%) as compared to healthy neighborhood controls (47.5%), although this difference was not statistically significant. Another three studies carried out in China (Cai et al, 2001;Jing et al, 2012) and Japan (Katoh et al, 1996) also reported high frequency of GSTM1 null genotype among stomach cancer cases than in controls. GSTT1 homozygous null genotype was 36.3% in cases and 45.6% in controls which difference was also not significant.…”

Section: Discussionmentioning

confidence: 92%

“…Although several meta-analyses revealed a strong relationship between H. pylori and gastric cancer (Eslick et al, 1999;Xue et al, 2001), it is still controversial in different Asian countries (Miwa et al, 2002;Jing et al, 2012). Various studies from India also failed to show an association between H. pylori infection and gastric cancer (Kate et al, 2000;Khanna et al, 2002;Singh et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Genetic Polymorphism of Glutathione S-transferases M1 and T1, Tobacco Habits and Risk of Stomach Cancer in Mizoram, India

Malakar¹,

Devi²,

Phukan³

et al. 2012

Aim: The incidence of stomach cancer in Mizoram is highest in India. We have conducted a population based matched case-control study to identify environmental and genetic risk factors in this geographical area. Methods: A total of 102 histologically confirmed stomach cancer cases and 204 matched healthy population controls were recruited. GSTM1 and GSTT1 genotypes were determined by PCR and H. pylori infections were determined by ELISA. Results: Tobacco-smoking was found to be an important risk factor for high incidence of stomach cancer in Mizoram. Meiziol (local cigarette) smoking was a more important risk factor than other tobacco related habits. Cigarette, tuibur (tobacco smoke infused water) and betel nut consumption synergistically increased the risk of stomach cancer. Polymorphisms of GSTM1 and GSTT1 genes were not found to be directly associated with stomach cancer in Mizoram. However, they appeared to be effect modifiers. Persons habituated with tobacco smoking and/or tuibur habit had increased risk of stomach cancer if they carried the GSTM1 null genotype and GSTT1 non-null genotype. Conclusion: Tobacco smoking, especially meiziol is the important risk factor for stomach cancer in Mizoram. GSTM1 and GSTT1 genes modify the effect of tobacco habits. This study is a first step in understanding the epidemiology of stomach cancer in Mizoram, India.