Glucosidase inhibitory activity and antioxidant activity of flavonoid compound and triterpenoid compound from Agrimonia Pilosa Ledeb

Abstract

BackgroundIn Chinese traditional medicine, Agrimonia pilosa Ledeb (APL) exhibits great effect on treatment of type 2 diabetes mellitus (T2DM), however its mechanism is still unknown. Considering that T2DM are correlated with postprandial hyperglycemia and oxidative stress, we investigated the α-glucosidase inhibitory activity and the antioxidant activity of flavonoid compound (FC) and triterpenoid compound (TC) from APL.MethodsEntire plants of APL were extracted using 95% ethanol and 50% ethanol successively. The resulting extracts were partitioned and isolated by applying liquid chromatography using silica gel column and Sephadex LH 20 column to give FC and TC. The content of total flavonoids in FC and the content of total triterpenoids in TC were determined by using UV spectrophotometry. HPLC analysis was used to identify and quantify the monomeric compound in FC and TC. The α-glucosidase inhibitory activities were determined using the chromogenic method with p-nitrophenyl-α-D-glucopyranoside as substrate. Antioxidant activities were assessed through three kinds of radical scavenging assays (DPPH radical, ABTS radical and hydroxyl radical) & β-carotene-linoleic acid assay.ResultsThe results indicate FC is abundant of quercitrin, and hyperoside, and TC is abundant of 1β, 2β, 3β, 19α-tetrahydroxy-12-en-28-oic acid (265.2 mg/g) and corosolic acid (100.9 mg/g). The FC & the TC have strong α-glucosidase inhibitory activities with IC50 of 8.72 μg/mL and 3.67 μg/mL, respectively. We find that FC show competitive inhibition against α-glucosidase, while the TC exhibits noncompetitive inhibition. Furthermore, The FC exhibits significant radical scavenging activity with the EC50 values of 7.73 μg/mL, 3.64 μg/mL and 5.90 μg/mL on DPPH radical, hydroxyl radical and ABTS radical, respectively. The FC also shows moderate anti-lipid peroxidation activity with the IC50 values of 41.77 μg/mL on inhibiting β-carotene bleaching.ConclusionThese results imply that the FC and the TC could be responsible for the good clinical effects of APL on T2MD through targeting oxidative stress and postprandial hyperglycaemia. So APL may be good sources of natural antioxidants and α-glucosidase inhibitors exhibiting remarkable potential value for the therapy of T2DM.

A diterpenoid sugiol from <i>Metasequoia glyptostroboides</i> with α-glucosidase and tyrosinase inhibitory potential

“…In this assay, the test compound sugiol showed inhibitory effect in a dose-dependent manner as did by the standard compound acarbose. Similar findings on α-glucosidase inhibitory activity of flavonoid and terpenoid compounds isolated from Agrimonia pilosa were observed by Liu et al (2014). In addition, sarcoviolins isolated from edible mushroom Sarcodon leucopus were also found to inhibit α-glucosidase in vitro (Ma et al, 2014).…”

“…Although there have been reports on the clinical availability of α-glucosidase inhibitors, there is still need to develop alternative therapies to inhibit this key enzyme in order to minimize side effects and drug cost efficacy. In this regard, several natural substances from plant origins have been analyzed for their enzymatic inhibitory activities (Tiwari et al, 2010;Ma et al, 2014;Liu et al, 2014).…”

Chard (Beta Vulgaris Var. Cicla) Extract Modulates Zinc Status, Glucose Level and Antioxydant Values in Diabetic Rats Fed Zinc Deficiency Diet

“…In other words, the findings showed a significant reduction in the formation of TBARS level and an augmentation of GSH concentration with an improvement of both GSH-Px and GST activities. Thus, it has been reported that several polyphenol compounds isolated from Beta vulgaris possess a strong antioxidant properties, which reduce the formation of ROS by directly inhibiting the reactive oxygen generating enzymes [31]. El-Gamal et al [55] indicated that chard treatment decreased the renal MDA levels and increased the CAT activity in gentamicin induced nephrotoxicity in rats.…”

“…They were found to be effective in controlling various biological activities. In other words, they are anti-inflammatory, anti-angiotic, antimicrobial, antioxidant, hypertension reduction and have anti-cholesterol properties [31]. In recent times, research interest has been paid to polyphenols and flavonoids as a result of their antioxidant capacity which is principally based on the redox properties of their hydroxyl groups and the structural relationship between different functional groups in their structure which enable them to actively serve as free radical scavengers, reducing agents, singlet oxygen quenchers metal chelators, and hydrogen donors [32].…”

HYTOCHEMICAL SCREENING, TOTAL FLAVONOID AND PHENOLIC CONTENT ASSAYS OF VARIOUS SOLVENT EXTRACTS OF TEPAL OF Musa paradisiaca

“…Flavonoids are proactive polyphenolic compounds found in most plants and cannot be synthesized or produced by the humans [37]. It was found to be effective in controlling various biological activities and is anti-inflammatory, anti-angiotic, antimicrobial, antioxidant, reduced hypertension, and has anti-cholesterol properties [38,39]. In recent times, research interest has been paid to polyphenols and flavonoids as a result of their antioxidant capacity which is principally based on the redox properties of their hydroxyl groups and the structural relationship between different functional groups in their structure which enable them to actively serve as free radical scavengers, reducing agents, singlet oxygen quenchers metal chelators, and hydrogen donors [40].…”