HYTOCHEMICAL SCREENING, TOTAL FLAVONOID AND PHENOLIC CONTENT ASSAYS OF VARIOUS SOLVENT EXTRACTS OF TEPAL OF Musa paradisiaca

Abstract

The objective of this research is to conduct the preliminary phytochemical screening, total flavonoid and phenolic contents assays of various solvent extracts of tepal of Musa paradisiaca. Phytochemical screening was carried out according to the method of Trease and Evans, total flavonoid content was measured by the aluminium chloride colorimetric assay and total phenolic content was estimated spectrophotometrically by Folin-Ciocalteau method. -Preliminary phytochemical screening reveals the presence of phenolics, flavonoids, alkaloids, tannins, terpenoids in all the three different extracts (methanolic, ethanolic and aqueous). Tepal methanolic extract has the richest content of both phenolics and flavonoids i.e. (4.27 mg GAE/g and 0.25 mg QE/g) respectively, and aqueous extract was the least i.e. (1.32 mg GAE/g and 0.164 mg QE/g). All the extracts were not significantly different with one another (p > 0.05). It can be hypothesised that the high contents of phenolic compounds of tepals of Musa paradisiaca indicated that these compounds contribute to the antioxidant activity and can be regarded as promising plant species for natural sources of radical scavenging activity with potential value for treatment of many life threatening diseases. Keywords: phytochemical, flavonoid, phenolics, Musa paradisiaca AbstrakObjektif kajian ini adalah untuk menjalankan pemeriksaan awal ke atas ketulenan fitokimia, jumlah kandungan fenolik flavonoid dan pelbagai ekstrak pelarut tepal Musa paradisiaca. Penyaringan fitokimia telah dilaksanakan mengikut kaedah Trease dan Evans, dimana jumlah kandungan flavonoid diukur oleh ujian kalorimetrik aluminium klorida dan jumlah kandungan fenolik dianggarkan secara spektrofotometer dengan kaedah Folin-Ciocalteau. Penyaringan awal fitokimia menunjukkan kehadiran fenolik, flavonoid, alkaloid, tannin dan terpenoid dalam ketiga-tiga ekstrak yang berbeza (metanol, etanol dan akueus). Ekstrak metanol Tepal mempunyai kandungan yang paling tinggi di antara kedua-dua fenolik dan flavonoid iaitu sebanyak 4.27 mg GAE/g dan 0.25 mg QE/g, manakala ekstrak akueus adalah mempunyai kandungan terendah iaitu sebanyak 1.32 mg GAE/g dan 0,164 mg QE/g. Semua ekstrak tidak berbeza secara ketara antara satu sama lain (p > 0.05). Oleh itu, hipotesis yang boleh dibuat adalah kandungan yang tinggi sebatian fenolik daripada tepal Musa paradisiaca menyumbang kepada aktiviti antioksidan dan boleh dianggap sebagai spesies tumbuhan yang berpotensi untuk merawat pelbagai penyakit yang mengancam nyawa.

Comparison of total phenols, flavonoids and antioxidant potential of local and imported ripe bananas

“…these biologically active compounds are generally soluble in H 2 O, hydroalcoholic and polar acetone solvent and gives sediments binding by proteins [19]. Several scientific pieces of evidence showed that phenolics were traditionally used for prevention of inflammation of the mouth and to treat wounds, hemorrhoids, and diarrhea [11,[20][21][22]. Plants, animals, and microorganisms are considered as the sources of natural product.…”

“…On the other hand, in Pilipino ripe banana samples, the maximum potential was in n-hexane and minimum in water followed by order of n-hexane > n-butanol > methanol > ethyl acetate > chloroform > water ( Table 5). From the literature, it has been obtained that the bananas crude extracts contain some primary active compound, e.g., tannin, glycosides, phenolic compounds, triterpenes, flavonoids, lanosterol, and a-amyrin [3,[8][9][10][11]22]. These biologically active compounds can able to neutralize the level of DPPH with color change by their hydrogen donating ability [15].…”

Characterization and antibacterial activity of cocos Nucifera L. Meat extract and powder as a drug and cosmetic agent

“…The methanol extract was iltered and 2 ml of sulfuric acid was added and was shaken to get 2 layers. The top layer (sulfate) was taken 5 drops, then 1 drop of Dragendorf reagent was added and the formation of orange deposits showed the presence of alkaloids (Rao and Mohd, 2016).…”

“…After being shaken for 30 seconds, changes occurred. If the permanent solid foam was formed (permanently for 30 seconds), this indicated the presence of saponins (Rao and Mohd, 2016).…”

“…A total of 0.50 ml of sample was dropped on a glass preparation, then 3 drops of methanol were added and were stirred until homogeneous, followed by the addition of 3 drops of 5% FeCl 3 . The formation of green, red, purple, or blue indicated the presence of phenolic compounds (Rao and Mohd, 2016).…”