Glucose oxidase overproduction by the mutant strain M-80.10 of Penicillium variabile in a benchtop fermenter

Petruccioli, Maurizio; Piccioni, Paola; Federici, Federico

doi:10.1016/s0141-0229(97)00024-0

Cited by 14 publications

(8 citation statements)

References 22 publications

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“…1) that, based on prediction analysis using the Psort software, should direct the intracellular expression of the protein. However, this prediction appears to be somehow in contrast with our previous results that demonstrated how the release of GOX activity into the culture broth by P. variabile P16 was not a consequence of mycelial cell lyses (Petruccioli et al. 1997).…”

Section: Isolation Of the Gox Gene Of P Variabile P16contrasting

confidence: 99%

“…In previous works, a strain (P16) of Penicillium variabile was selected which released high levels of glucose oxidase activity into its culture medium (Petruccioli et al. 1993) and the enzyme production optimized at the bench‐fermenter scale (Petruccioli et al. 1995, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…In previous works, a strain (P16) of Penicillium variabile was selected which released high levels of glucose oxidase activity into its culture medium (Petruccioli et al 1993) and the enzyme production optimized at the bench-fermenter scale (Petruccioli et al , 1997. The enzyme was identified as a dimeric flavoprotein with a relative molecular mass of 126 000 Da with an optimum for activity at pH 6AE0 and 55°C.…”

Section: Introductionmentioning

confidence: 99%

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The glucose oxidase of Penicillium variabile P16: gene cloning, sequencing and expression

Pulci

D’Ovidio

Petruccioli

et al. 2004

Lett Appl Microbiol

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Aims: Isolation and characterization of the glucose oxidase (GOX)‐encoding gene from a Penicillium variabile strain (P16) having a high level of GOX activity and comparison of its expression with that of another strain of P. variabile (NRRL 1048) characterized by low GOX activity. Methods and Results: The gene, isolated by PCR consisted of 1818 bp encoding 605 amino acid residues. Gene expression was analysed by Northern blotting and compared with that of P. variabile NRRL 1048. The higher GOX activity of strain P16 appeared likely because of de novo mRNA synthesis. Southern blotting analyses of the genomic DNA showed that the hybridization pattern of the two strains differed for the size of hybridizing fragment detected by the probe and slightly for their signal intensity. Conclusions: The GOX‐encoding gene of P. variabile P16 was isolated and characterized to identify the molecular bases of its high level of expression and in view of improving enzyme production by developing a process based on heterologous expression. Significance and Impact of the Study: GOX‐encoding genes can be subjected to high difference in their expression levels. The P16 strain of P. variable producing large amount of GOX as well as its encoding gene might be exploited for industrial applications.

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Section: Isolation Of the Gox Gene Of P Variabile P16contrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The glucose oxidase of Penicillium variabile P16: gene cloning, sequencing and expression

Pulci

D’Ovidio

Petruccioli

et al. 2004

Lett Appl Microbiol

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“…Enzymes derived from cloned genes of GMO organisms may also be involved in the production of gluconic acid production or alternative economical host systems can be developed [7,15,21,22]. Strain improvement has been reported to be an essential step in developing industrial microbial production processes [23].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Iron on Gluconic Acid Production by Aureobasidium pullulans

Anastassiadis¹,

Kamzolova²,

Morgunov³

et al. 2008

TOBIOTJ

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New processes have been previously described for the continuous and discontinuous production of gluconic acid by Aureobasidium pullulans (de bary) Arnaud. Little is known about the regulatory mechanisms of gluconic acid production by A. pullulans. The response of growth and gluconic acid metabolism to a variable profile of iron concentrations was studied with A. pullulans in batch and chemostat cultures. A surprisingly high optimum N-dependent iron ion concentration in the feed medium, in the range between 0.5 mM and 3.0 mM Fe (optimum 1-2 mM), was found to be particular requirement for economically profitable continuous production of gluconic acid with 3 g/l NH 4 Cl. Increased iron concentration promoted growth on defined glucose medium. 223.3 g/l gluconic acid were continuously produced at a formation rate of the generic product (R j ) of 16.8 g/(l h) and a specific gluconic acid productivity (m p ) of 2.5 g/(g h) at 13 h residence time (RT) with 1mM iron, compared with 182 g/l reached at 0.1 mM. The product selectivity (product yield based on glucose) increased continuously by raising iron concentration following a saturation curve, reaching a maximum of about 98% (mol/mol) at 2 mM Fe and 76.2% conversion, compared with only 84.3% determined at 0.1 mM. The process is not obligatory growth limiting or growth related and residual nitrogen was found in all of continuous experiments, e.g. 197 mg/l of nitrogen at 0.1 mM and 201 mg/l at 2 mM of iron.

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“…30,32 For commercial applications, glucose oxidase has been produced and purified from fungi, especially from Aspergillus and Penicillium species. 19,[33][34][35][36][37][38][39][40][41][42][43][44][45] The great success of these two species is mainly due to their metabolic versatility and their recognition as GRAS (generally recognized as safe). 19,38 Among the Aspergillus species, remarkable attention has been given to A. niger over the last 60 years.…”

Section: Catalysis Science and Technology Mini Reviewmentioning

confidence: 99%

Enzyme production ofd-gluconic acid and glucose oxidase: successful tales of cascade reactions

Kornecki

Carballares

Tardioli

et al. 2020

Catal. Sci. Technol.

102

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Glucose oxidase overproduction by the mutant strain M-80.10 of Penicillium variabile in a benchtop fermenter

Cited by 14 publications

References 22 publications

The glucose oxidase of Penicillium variabile P16: gene cloning, sequencing and expression

The glucose oxidase of Penicillium variabile P16: gene cloning, sequencing and expression

The Effect of Iron on Gluconic Acid Production by Aureobasidium pullulans

Enzyme production ofd-gluconic acid and glucose oxidase: successful tales of cascade reactions

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