Glucose oxidase and Au nanocluster co-encapsulated metal–organic frameworks as a sensitive colorimetric sensor for glucose based on a cascade reaction

Abstract

<p>In this work, glucose oxidase and Au nanoclusters co-encapsulated zeolitic imidazolate frameworks (GOx&amp;AuNCs@ZIF-8) were obtained by simply mixing Zn2+, dimethylimidazole, glucose oxidase (GOx) and Au nanoclusters (AuNCs) at room temperature....</p>

Metal–organic framework (MOF) hybridized gold nanoparticles as a bifunctional nanozyme for glucose sensing

“…The V max , K m , and K w /K m values of AuNPs@PCN-224(Fe) were determined to be 0.06 mM s −1 , 0.52 mM and 11.54 × 10 −5 M s −1 L g −1 , respectively. A comparison to previously known nanozymes with dual-catalytic activities indicates that our hybrid MOF is among the more active in terms of glucose sensing (Table S2 †), [72][73][74][75] because the majority of previously developed nanozymes require the presence of GOx to achieve the cascade reaction, whereas our AuNPs@PCN-224(Fe) is completely natural enzyme-free.…”

Challenges and Opportunities of Using Fluorescent Metal Nanocluster-Based Colorimetric Assays in Medicine

“…117 J. Chi et al developed a colorimetric system with an improved peroxidase-like activity using Au, GOx, and zeolitic imidazolate frameworks (AuNCs@ZIF-8) which could detect glucose by the produced H 2 O 2 from the reaction between glucose and GOx. 116 J. Feng et al fabricated gold−platinum nanoclusters with an optimal Au/Pt molar ratio (1:1) by a onepot synthetic route. The NCs-GOx cascade-catalyzed system using TMB enabled sensitive and selective glucose detection.…”

Metal–Organic Frameworks-Mediated Assembly of Gold Nanoclusters for Sensing Applications

“…These outstanding performances of MOF/AuNCs assemblies facilitate the sensitive colorimetric sensing of organophosphorus pesticides (OPs), acetylcholinesterase (AChE), choline oxidase (CHO), glucose, hydrogen peroxide, etc. [ 70 , 71 , 121 – 123 ].…”

“…The enzyme cascade of HRP and GOx has been extensively applied to the detection of glucose, and the detection is sensitive and selective benefiting from the specific catalysis on glucose by GOx [ 127 ]. For example, BSA-protected AuNCs and GOx were encapsulated by ZIF-8, which could sensitively and selectively detect glucose via colorimetric sensing on the basis of cascade reaction [ 121 ]. Under the enzymolysis of GOx, glucose was decomposed to gluconic acid accompanied with H 2 O 2 generation, which acted as a substrate for HRP-mimicking catalysis of AuNCs to oxidize colorless TMB to blue oxTMB with an absorption peak at 652 nm.…”