Glucose obtained from rice bran by ultrasound-assisted enzymatic hydrolysis

Abstract

<p>In this work ultrasound-assisted solid-state enzymatic hydrolysis of rice bran to obtain fermentable sugars was investigated. For this purpose, process variables such as temperature, enzyme concentration and moisture content were evaluated during the enzymatic hydrolysis with and without ultrasound irradiation. The enzyme used is a blend of amylases derived from genetically modified strains of <em>Trichoderma</em> <em>reesei</em>. Kinetic of the enzymatic hydrolysis of rice bran at the constant-reaction rate period were measured. The best results for the ultrasound-assisted enzymatic hydrolysis was obtained using 3 wt% of enzyme, 60 <sup>o</sup>C and moisture content of 65 wt%, yielding 0.38 g sugar/g rice bran, whereas for the hydrolysis in the absence of ultrasound the highest yield was 0.20 g sugar/g rice bran using 3 wt% of enzyme, 60 <sup>o</sup>C and moisture content of 50 wt%. The use of ultrasound-assisted enzymatic hydrolysis of rice bran was intensified, obtaining around 74% more fermentable sugar than in the absence, showing that the use of ultrasound is a promising technology to be used in enzymatic reaction as an alternative of process intensification. </p>

Utilization of Indonesian root and tuber starches for glucose production by cold enzymatic hydrolysis

“…This enzyme is an optimized blend of Aspergillus kawachi α-amylase and a glucoamylase from Trichoderma reesei (Genencor 2008). To date, most research has been conducted on Stargen™ 002 to improve the liquefaction and saccharification steps in the glucose and bioethanol production from grain crops such as corn, rice, and wheat starch (Adams et al 2011;Chu-Ky et al 2015;Kuhn et al 2015;Strąk-Graczyk et al 2019;Tse et al 2021) or cassava (Krajang et al 2021;Tse et al 2021). To our knowledge, the information available on the enzymatic saccharification of tropical raw starches using Stargen™ 002 at below gelatinization temperature is still limited (Hargono et al 2018;Krajang et al 2021).…”