Glucose movement into rat uterine fluid

Leese, Henry J.; Aldridge, Sue; Kiernan, Theresa

doi:10.1530/jrf.0.0560615

Cited by 7 publications

(3 citation statements)

References 5 publications

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“…It has been shown in the normal rat that glucose reaches the concentration of 1 mmol/1 in the uterine lumen (53) and that the glucose content found in the uterine fluid of alloxan-treated diabetic mice is four times higher than that in control mice at the time of implantation (54). It has been proposed that abnormal levels of glucose are central to the development of uterine vascularization and decidualization deficiencies observed in the pregnant uterus of alloxan-diabetic rats (55).…”

Section: Discussionmentioning

confidence: 99%

Increased Synthesis of Tumor Necrosis Factor-α in Uterine Explants From Pregnant Diabetic Rats and in Primary Cultures of Uterine Cells in High Glucose

1997

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The production of tumor necrosis factor-alpha (TNF-alpha) was investigated in uterine explants from normal, diabetic, or insulin-treated diabetic pregnant rats. Explants from diabetic rats released more soluble TNF-alpha than did those in the other groups. The extent of this secretion was correlated with blood glucose concentration at the time of explantation. The concentration of cell membrane-associated TNF-alpha in the explants was not altered by diabetes. Daily insulin administration failed to normalize uterine TNF-alpha secretion despite correction of glycemia in the diabetic rats. Explants from normal pregnant rats cultured in vitro with increasing concentrations of D-glucose showed a dose-dependent increase in TNF-alpha secretion. The production of TNF-alpha in high glucose was also tested in primary cultures of uterine cells isolated from either immature or adult rats. TNF-alpha secretion was increased in high D-glucose but not in iso-osmolar concentrations of L-glucose, D-raffinose, D-galactose, or mannitol. Cell membrane-associated TNF-alpha was not influenced by high D-glucose. Semiquantitative reverse transcription-amplification of RNA extracted from primary cultures of uterine cells showed that the steady-state level of TNF-alpha transcripts was increased by high D-glucose but not by high L-glucose. The results are consistent with the hypothesis that hyperglycemia is instrumental in the overexpression of TNF-alpha in the diabetic uterus. Because TNF-alpha has a demonstrated negative impact on embryonic growth, enhanced TNF-alpha synthesis in the pregnant uterus may contribute to the embryopathy associated with maternal diabetes.

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Section: Discussionmentioning

confidence: 99%

Increased Synthesis of Tumor Necrosis Factor-α in Uterine Explants From Pregnant Diabetic Rats and in Primary Cultures of Uterine Cells in High Glucose

1997

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“…The blood-uterine lumen barrier also has tEe ability to transport a variety of low molecularweight electrolytes and non-electrolytes including potassium (Levin & Edwards, 1968), amino acids (Walters et al, 1979(Walters et al, , 1981, glucose and certain other monosaccharide energy substrates (Leese et al 1979) and possibly prostaglandins (see below) (Jones & Harper, 1983;Cao et al, 1984). For example, the potassium ion concentration in uterine fluid from rat pro-oestrous fluid, which accumulates copiously, is about 10-fold greater than that in plasma (Howard & DeFeo, 1959).…”

Section: Permeability and Transport Properties Of Uterine Epitheliummentioning

confidence: 99%

The blood-uterine lumen barrier and exchange between extracellular fluids

McRae¹

1988

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Summary. The concept of a blood-tissue barrier defines the rates at which matter exchanges among the vascular and extravascular fluids of the tissue. The remarkably slow rates at which substances such as mannitol ( M , 182) enter uterine fluid from plasma demonstrate the existence of a blood-uterine lumen barrier. Available evidence indicates that the uterine microvascular endothelium and the uterine epithelium behave as lipoid layers interrupted by water-filled channels. Furthermore, both cell layers appear to select actively certain substances over others for exchange with opposing extracellular fluids. In contrast to these similarities, the uterine epithelium and endothelium differ considerably with regard to restrictiveness. For most substances the primary rate-limiting boundary between blood and the uterine lumen is the epithelium. The extracellular fluid compartments of the lumen and endometrium are also influenced by the internalization and release of materials into and out of intracellular compartments including those of the stromal and migratory cells of the endometrium, the epithelium and the developing conceptus. Considerable evidence suggests that the luminal milieu of the developing embryo is created and maintained by the transport and permeability properties o r the blood-uterine lumen barrier in conjunction with the cellular activities of the endometrium and embryo. This milieu probably fulfils the informational and nutritional needs of the developing embryo.

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“…The concentrations of fructose and spermatozoa in the rat uterus after mating were therefore measured. Since glucose is readily secreted into the perfused rat uterine lumen (Leese, Aldridge & Kiernan, 1979), the concentration of this sugar was also measured.…”

Section: Introductionmentioning

confidence: 99%