The blood-uterine lumen barrier and exchange between extracellular fluids

McRae, Ann C.

doi:10.1530/jrf.0.0820857

Cited by 30 publications

(17 citation statements)

References 95 publications

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“…the time when sows develop the syndrome (Middleton-Williams et al, 1977;Wegman, 1985). Sex hormones may also in¯uence the resistance to infections by changing the adherence ability of epithelial cells (Sobel and Kaye, 1986 2 ), the epithelium permeability (McRae, 1988) and the environment for the microbe, e.g. the composition of mammary secretions (Cowie, 1984).…”

Section: Introductionmentioning

confidence: 99%

Sows Intramammarily Inoculated with Escherichia coli: Influence of Time of Infection, Hormone Concentrations and Leucocyte Numbers on Development of Disease

et al. 2001

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The aim of this study was to identify factors that influence the development of disease in sows inoculated with Escherichia coli in the mammary gland. Ten cross-bred primiparous sows were intramammarily inoculated with living E. coli bacteria at different time points before parturition: seven sows within 48 h before parturition and three sows approximately 96 h before parturition. Before and after inoculation, blood samples and mammary gland biopsy specimens were collected and clinical observations were made. All seven sows inoculated close to parturition developed a rectal temperature of >39.5 degrees C during the first 48 h post-partum and two of them also showed other signs of clinical disease. In the sows inoculated 4 days before parturition, the rectal temperature never exceeded 39.5 degrees C during the first 48 h post-partum and none of them showed any other sign of clinical discase. There was a tendency (P < 0.1) that histological signs of mastitis were more frequent in the sows inoculated close to parturition. There were no overall differences between the two groups of sows in plasma concentrations of cortisol, oestradiol-17beta and 15-ketodihydro-PGF2alpha before inoculation. Before inoculation, the number of neutrophils in the blood was overall higher (P < 0.05) in the group of sows that were inoculated close to parturition. In comparison, the number of lymphocytes before inoculation had a tendency (P < 0.1) to be lower in that group. The data suggest that the time of infection of the mammary gland relative to parturition and the number of circulating neutrophils at the time of infection influence the development of chinical coliform mastitis in the sow.

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Section: Introductionmentioning

confidence: 99%

Sows Intramammarily Inoculated with Escherichia coli: Influence of Time of Infection, Hormone Concentrations and Leucocyte Numbers on Development of Disease

et al. 2001

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“…The uterine luminal environment is separated from the surrounding uterine tissue by a permeability barrier which exhibits complex behaviour towards blood-borne substances (McRae & Kennedy, 1981, 1983a and seems to function more broadly than the simple exclusion of certain substances from the uterine lumen (see McRae, 1984McRae, , 1988, for reviews). The composition of this environment at any given time will depend upon a balance between the influx and efflux of material across the luminal and glandular epithelia.…”

Section: Introductionmentioning

confidence: 99%

Uterine vascular permeability, blood flow and extracellular fluid space during implantation in rats

McRae

Heap²

1988

Reproduction

Self Cite

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Vascular permeability to plasma proteins in uterine implantation and non-implantation sites (i.e. dye sites and non-dye sites) was assessed quantitatively by a method which accounts for steady-state volumes of distribution. Extracellular fluid volume and uterine blood flow were also determined. On both the evening of Day 5 and the morning of Day 6, vascular permeability to 125I-labelled human serum albumin, extracellular fluid volume and blood flow were significantly increased in implantation sites compared to non-implantation sites. Vascular permeability in implantation sites was increased significantly between Days 5 and 6, whereas that in non-implantation sites was unchanged. This increase in vascular permeability between Days 5 and 6 was not accompanied by further increases in extracellular fluid volume and blood flow. This result shows a dissociation between vascular permeability and extracellular fluid volume immediately after the onset of implantation and raises important questions as to whether the rat uterus undergoes a truly oedematous response at implantation as has been generally accepted.

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“…As a transporting and secretory epithelium, UEC regulate the ionic and macromolecular traffic between lumenal and stromal compartments, serving as a contributor and regulator of fluid phase contents in both compartments (McRae, 1988). As is true for many epithelia (Milks et al, 1983), leukocytes can penetrate the uterine epithelial layer without disrupting it; however, this cell movement occurs from the stromal compartment through to the uterine lumen (i.e., in a basal to apical direction) (Wilson and Potts, 1970).…”

Section: Discussionmentioning

confidence: 99%

WGA‐binding, mucin glycoproteins protect the apical cell surface of mouse uterine epithelial cells

Valdizan

Julian

Carson

1992

Journal Cellular Physiology

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Expression of apical cell surface proteins and glycoproteins was examined in polarized primary cultures of mouse uterine epithelial cells (UEC). Lectin-gold cytochemistry revealed that wheat germ agglutinin (WGA) bound specifically to the components of the apical glycocalyx as well as intracellular vesicles. Double labeling with the pH sensitive dye 3-(2,4-dinitroanilino)-3'amino-N-methyldipropylamine (DAMP) demonstrated the acidic nature of the WGA-staining intracellular vesicles. The enzymatic and chemical sensitivities of the WGA binding sites on the apical cell surface were monitored both by WGA-gold staining as well as by 125I-WGA binding assays. In thin sections, a large fraction of these sites were removed by pronase; however, application of a wide variety of proteases, glycosidases, or chemical treatments to the apical surface of intact UEC failed to reduce WGA binding. In no case did treatments designed to remove sialic acids reduce 125I-WGA binding more than 12%. In contrast, endo-beta-galactosidase as well as a combination of beta-galactosidase with beta-hexosaminidase succeeded in removing 28% and 77% of these sites, respectively. These studies suggested that the majority of the apically disposed WGA binding sites involved N-acetylglucosamine residues rather than sialic acids and included lactosaminoglycans. Many of the proteins detected at the apical cell surface by lactoperoxidase-catalyzed radioiodination were WGA-binding glycoproteins. A major class of these glycoproteins displayed Mr > 200 kDa by SDS-PAGE and was heavily labeled metabolically by 3H-glucosamine or by vectorial labeling at the apical cell surface with galactosyl transferase and UDP-3H-galactose. Analyses of the 3H-labeled oligosaccharides labeled by either procedure indicated that a large fraction of the apically disposed WGA-binding oligosaccharides consisted of neutral, O-linked mucin-type structures with median MW of approximately 1,500. Oligosaccharides in this fraction were partially (15%) sensitive to endo-beta-galactosidase digestion and bound to Datura stramonium agglutinin (68%), demonstrating the presence of lactosaminoglycan sequences. UEC were an extremely effective barrier to attachment or invasion by either a highly invasive melanoma cell line, B16-BL6, or implantation-competent mouse blastocysts. In contrast, neither uterine stromal cells nor a non-polarizing UEC cell line, RL95, prevented B16-BL6 attachment.(ABSTRACT TRUNCATED AT 400 WORDS)

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The blood-uterine lumen barrier and exchange between extracellular fluids

Cited by 30 publications

References 95 publications

Sows Intramammarily Inoculated with Escherichia coli: Influence of Time of Infection, Hormone Concentrations and Leucocyte Numbers on Development of Disease

Sows Intramammarily Inoculated with Escherichia coli: Influence of Time of Infection, Hormone Concentrations and Leucocyte Numbers on Development of Disease

Uterine vascular permeability, blood flow and extracellular fluid space during implantation in rats

WGA‐binding, mucin glycoproteins protect the apical cell surface of mouse uterine epithelial cells

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